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01-04-2016 | Original Article

Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer

Authors: Htoo Zarni Oo, Kazuhiro Sentani, Shoichiro Mukai, Takuya Hattori, Shunsuke Shinmei, Keisuke Goto, Naoya Sakamoto, Yutaka Naito, Katsuhiro Anami, Pharm Thi Binh Trang, Kazuyoshi Yanagihara, Naohide Oue, Wataru Yasui

Published in: Gastric Cancer | Issue 2/2016

Abstract

Background

Gastric cancer (GC) is the fifth commonest malignancy worldwide and still one of the leading causes of cancer-related death. The aim of this study was to identify a novel prognostic marker or therapeutic target for GC.

Methods

We analyzed candidate genes from our previous Escherichia coli ampicillin secretion trap (CAST) libraries in detail, and focused on the FKTN gene because it was overexpressed in both GC cell line CAST libraries, MKN-1 and MKN-45.

Results

Quantitative reverse transcriptase PCR analysis of FKTN revealed that FKTN messenger RNA was overexpressed in nine of 28 (32.1 %) GC tissue samples compared with nonneoplastic gastric mucosa. Immunostaining of fukutin showed that 297 of 695 cases (42.7 %) were positive for fukutin. Fukutin-positive GC cases were significantly associated with differentiated histological features, and advanced T grade and N grade. In addition, fukutin expression was observed more frequently in the intestinal phenotype (51 %) of GC than in other phenotypes (37 %) when defined by the expression patterns of mucin 5AC, mucin 6, mucin 2, and CD10. FKTN small interfering RNA treatment decreased GC cell proliferation.

Conclusions

These results indicate that the expression of fukutin may be a key regulator for progression of GC with the intestinal mucin phenotype.

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Title: Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer
Authors: Htoo Zarni Oo
Kazuhiro Sentani
Shoichiro Mukai
Takuya Hattori
Shunsuke Shinmei
Keisuke Goto
Naoya Sakamoto
Yutaka Naito
Katsuhiro Anami
Pharm Thi Binh Trang
Kazuyoshi Yanagihara
Naohide Oue
Wataru Yasui
Publication date: 01-04-2016
Publisher: Springer Japan
Published in: Gastric Cancer / Issue 2/2016
Print ISSN: 1436-3291
Electronic ISSN: 1436-3305
DOI: https://doi.org/10.1007/s10120-015-0511-2

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Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer

Abstract

Background

Methods

Results

Conclusions

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Abstract

Background

Methods

Results

Conclusions

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