Top

Published in:

01-10-2020 | Vitiligo | Original Article

42 °C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro

Authors: Wenzhi Hu, Na Mi, Yanfang Xu, Guang Zhao, Weijie Gu

Published in: Lasers in Medical Science | Issue 8/2020

Abstract

Vitiligo is a common depigment of skin disorder due to loss of functional melanocytes. Recently, the phototherapy with a 308-nm xenon-chloride excimer laser (UVB laser) is wildly used in vitiligo treatment. However, excessive UVB will induce photo-damage and photo-carcinogenesis in melanocytes. Previous studies revealed a protective effect of heat on UVB-induced melanocyte damage. In this study, we combined heat stress pretreatment with UVB to evaluate whether heat stress pretreatment has an ameliorative effect on UVB-induced damage. Human primary melanocytes (HMCs) were cultured and irradiated with a 308-nm laser with/without heat treatment. MTT assay, apoptosis analysis, and comet assay were conducted to monitor the damage of HMCs. Western blot and immunofluorescence staining were performed to assess the expression and subcellular localization of HSP70. HMCs heated at 42 °C for 1 h exhibit no cytotoxicity. Furthermore, preheat treatment attenuated the UVB laser-induced injury, reduced the DNA damage, and attenuated the cell apoptosis. The level and the localization of HSP70 determined the protective effects against UVB-induced DNA damage. Combining preheat treatment with a 308-nm xenon-chloride excimer laser would be a potential therapeutic method not only promotes the repigment of vitiligo but also reduces the UVB-induced photo-damage.

Available only for authorised users

Bishnoi A, Parsad D (2018) Clinical and molecular aspects of vitiligo treatments [J]. Int J Mol Sci 19(5): 1-15

Beggs S, Short J, Rengifo-Pardo M, Ehrlich A (2015) Applications of the excimer laser: a review [J]. Dermatol Surg 41(11):1201–1211CrossRef

Mohania D, Chandel S, Kumar P, Verma V, Digvijay K, Tripathi D, Choudhury K, Mitten SK, Shah D (2017) Ultraviolet radiations: skin defense-damage mechanism [J]. Adv Exp Med Biol 996:71–87CrossRef

Nakazawa K, Sahuc F, Damour O, Collombel C, Nakazawa H (1998) Regulatory effects of heat on normal human melanocyte growth and melanogenesis: comparative study with UVB [J]. J Invest Dermatol 110(6):972–977CrossRef

Gu WJ, Ma HJ, Zhao G, Yuan XY, Zhang P, Liu W, Ma LJ, Lei XB (2014) Additive effect of heat on the UVB-induced tyrosinase activation and melanogenesis via ERK/p38/MITF pathway in human epidermal melanocytes [J]. Arch Dermatol Res 306(6):583–590CrossRef

Gu Y, Chen J, Wang T, Zhou C, Liu Z, Ma L (2016) Hsp70 inducer, 17-allylamino-demethoxygeldanamycin, provides neuroprotection via anti-inflammatory effects in a rat model of traumatic brain injury [J]. Exp Ther Med 12(6):3767–3772CrossRef

Kiemer AK, Gerbes AL, Bilzer M, Vollmar AM (2002) The atrial natriuretic peptide and cGMP: novel activators of the heat shock response in rat livers [J]. Hepatology (Baltimore, Md) 35(1):88–94CrossRef

Kuboki S, Schuster R, Blanchard J, Pritts TA, Wong HR, Lentsch AB (2007) Role of heat shock protein 70 in hepatic ischemia-reperfusion injury in mice [J]. Am J Physiol Gastrointest Liver Physiol 292(4):G1141–G1149CrossRef

Tashiro S, Miyake H, Rokutan K (2018) Role of geranylgeranylacetone as non-toxic HSP70 inducer in liver surgery: clinical application [J]. J Hepatobiliary Pancreat Sci 25(5):269–274CrossRef

10.

Tanimoto T, Parseghian MH, Nakahara T, Kawai H, Narula N, Kim D, Nishimura R, Weisbart RH, Chan G, Richieri RA, Haider N, Chaudhry F, Reynolds GT, Billimek J, Blankenberg FG, Sengupta PP, Petrov AD, Akasaka T, Strauss HW, Narula J (2017) Cardioprotective effects of HSP72 administration on ischemia-reperfusion injury [J]. J Am Coll Cardiol 70(12):1479–1492CrossRef

11.

Ateş GB, Ak A, Garipcan B, Gülsoy M (2018) Indocyanine green-mediated photobiomodulation on human osteoblast cells [J]. Lasers Med Sci 33(7):1591–1599CrossRef

12.

Konca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Gozdz S, Koza Z, Wojcik A (2003) A cross-platform public domain PC image-analysis program for the comet assay [J]. Mutat Res 534(1–2):15–20CrossRef

13.

Ramsby ML, Makowski GS, Khairallah EA (1994) Differential detergent fractionation of isolated hepatocytes: biochemical, immunochemical and two-dimensional gel electrophoresis characterization of cytoskeletal and noncytoskeletal compartments [J]. Electrophoresis 15(2):265–277CrossRef

14.

Palleros DR, Welch WJ, Fink AL (1991) Interaction of hsp70 with unfolded proteins: effects of temperature and nucleotides on the kinetics of binding [J]. Proc Natl Acad Sci U S A 88(13):5719–5723CrossRef

15.

Kampinga HH, Craig EA (2010) The HSP70 chaperone machinery: J proteins as drivers of functional specificity [J]. Nat Rev Mol Cell Biol 11(8):579–592CrossRef

16.

Clerico EM, Tilitsky JM, Meng W, Gierasch LM (2015) How hsp70 molecular machines interact with their substrates to mediate diverse physiological functions [J]. J Mol Biol 427(7):1575–1588CrossRef

17.

Rudiger S, Buchberger A, Bukau B (1997) Interaction of Hsp70 chaperones with substrates [J]. Nat Struct Biol 4(5):342–349CrossRef

18.

Tavaria M, Gabriele T, Kola I, Anderson RL (1996) A hitchhiker’s guide to the human Hsp70 family [J]. Cell Stress Chaperones 1(1):23–28CrossRef

19.

Chung J, Nguyen AK, Henstridge DC, Holmes AG, Chan MH, Mesa JL, Lancaster GI, Southgate RJ, Bruce CR, Duffy SJ, Horvath I, Mestril R, Watt MJ, Hooper PL, Kingwell BA, Vigh L, Hevener A, Febbraio MA (2008) HSP72 protects against obesity-induced insulin resistance [J]. Proc Natl Acad Sci U S A 105(5):1739–1744CrossRef

20.

Pratt WB, Gestwicki JE, Osawa Y, Lieberman AP (2015) Targeting Hsp90/Hsp70-based protein quality control for treatment of adult onset neurodegenerative diseases [J]. Annu Rev Pharmacol Toxicol 55:353–371CrossRef

21.

Levada K, Guldiken N, Zhang X, Vella G, Mo FR, James LP, Haybaeck J, Kessler SM, Kiemer AK, Ott T, Hartmann D, Huser N, Ziol M, Trautwein C, Strnad P (2018) Hsp72 protects against liver injury via attenuation of hepatocellular death, oxidative stress, and JNK signaling [J]. J Hepatol 68(5):996–1005CrossRef

22.

Bivik C, Rosdahl I, Ollinger K (2007) Hsp70 protects against UVB induced apoptosis by preventing release of cathepsins and cytochrome c in human melanocytes [J]. Carcinogenesis 28(3):537–544CrossRef

23.

Stankiewicz AR, Lachapelle G, Foo CP, Radicioni SM, Mosser DD (2005) Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation [J]. J Biol Chem 280(46):38729–38739CrossRef

24.

Li H, Liu L, Xing D, Chen WR (2010) Inhibition of the JNK/Bim pathway by Hsp70 prevents Bax activation in UV-induced apoptosis [J]. FEBS Lett 584(22):4672–4678CrossRef

25.

Schmitt E, Gehrmann M, Brunet M, Multhoff G, Garrido C (2007) Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy [J]. J Leukoc Biol 81(1):15–27CrossRef

26.

Ruchalski K, Mao H, Li Z, Wang Z, Gillers S, Wang Y, Mosser DD, Gabai V, Schwartz JH, Borkan SC (2006) Distinct hsp70 domains mediate apoptosis-inducing factor release and nuclear accumulation [J]. J Biol Chem 281(12):7873–7880CrossRef

27.

Beere HM, Wolf BB, Cain K, Mosser DD, Mahboubi A, Kuwana T, Tailor P, Morimoto RI, Cohen GM, Green DR (2000) Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome [J]. Nat Cell Biol 2(8):469–475CrossRef

28.

Senf SM, Dodd SL, McClung JM, Judge AR (2008) Hsp70 overexpression inhibits NF-kappaB and Foxo3a transcriptional activities and prevents skeletal muscle atrophy [J]. FASEB J 22(11):3836–3845CrossRef

29.

Chen H, Wu Y, Zhang Y, Jin L, Luo L, Xue B, Lu C, Zhang X, Yin Z (2006) Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination [J]. FEBS Lett 580(13):3145–3152CrossRef

30.

Matsuda M, Hoshino T, Yamashita Y, Tanaka K, Maji D, Sato K, Adachi H, Sobue G, Ihn H, Funasaka Y, Mizushima T (2010) Prevention of UVB radiation-induced epidermal damage by expression of heat shock protein 70 [J]. J Biol Chem 285(8):5848–5858CrossRef

31.

Kayama M, Nakazawa T, Thanos A, Morizane Y, Murakami Y, Theodoropoulou S, Abe T, Vavvas D, Miller JW (2011) Heat shock protein 70 (HSP70) is critical for the photoreceptor stress response after retinal detachment via modulating anti-apoptotic Akt kinase [J]. Am J Pathol 178(3):1080–1091CrossRef

32.

Lennikov A, Kitaichi N, Kase S, Noda K, Horie Y, Nakai A, Ohno S, Ishida S (2013) Induction of heat shock protein 70 ameliorates ultraviolet-induced photokeratitis in mice [J]. Int J Mol Sci 14(1):2175–2189CrossRef

33.

Svobodova A, Walterova D, Vostalova J (2006) Ultraviolet light induced alteration to the skin [J]. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150(1):25–38CrossRef

34.

Glady A, Tanaka M, Moniaga CS, Yasui M, Hara-Chikuma M (2018) Involvement of NADPH oxidase 1 in UVB-induced cell signaling and cytotoxicity in human keratinocytes [J]. Biochem Biophys Rep 14:7–15PubMedPubMedCentral

35.

Yurinskaya MM, Kochetkova OY, Shabarchina LI, Antonova OY, Suslikov AV, Evgen’ev MB, Vinokurov MG (2017) Encapsulated Hsp70 decreases endotoxin-induced production of ROS and TNFalpha in human phagocytes [J]. Cell Stress Chaperones 22(1):163–171CrossRef

36.

Gilbert CS, van den Bosch M, Green CM, Vialard JE, Grenon M, Erdjument-Bromage H, Tempst P, Lowndes NF (2003) The budding yeast Rad9 checkpoint complex: chaperone proteins are required for its function [J]. EMBO Rep 4(10):953–958CrossRef

37.

Kotoglou P, Kalaitzakis A, Vezyraki P, Tzavaras T, Michalis LK, Dantzer F, Jung JU, Angelidis C (2009) Hsp70 translocates to the nuclei and nucleoli, binds to XRCC1 and PARP-1, and protects HeLa cells from single-strand DNA breaks [J]. Cell Stress Chaperones 14(4):391–406CrossRef

38.

Liu-Smith F, Jia J, Zheng Y (2017) UV-induced molecular signaling differences in melanoma and non-melanoma skin cancer [J]. Advances in experimental medicine and biology 996:27–40CrossRef

Title: 42 °C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro
Authors: Wenzhi Hu
Na Mi
Yanfang Xu
Guang Zhao
Weijie Gu
Publication date: 01-10-2020
Publisher: Springer London
Keywords: Vitiligo
Laser
Published in: Lasers in Medical Science / Issue 8/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03012-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

42 °C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 8/2020

Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway

The value of transurethral thulium laser en bloc resection combined with a single immediate postoperative intravesical instillation of pirarubicin in primary non-muscle-invasive bladder cancer

Laser treatment in retinitis pigmentosa-a review

Sensory and motor responses after photobiomodulation associated with physiotherapy in patients with incomplete spinal cord injury: clinical, randomized trial

Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner

Effects of consumption of contaminated feed with 2,4-dichlorophenoxyacetic acid (2,4-D) on the rat tibia: analysis by Raman spectroscopy and mechanical properties