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01-10-2020 | Phototherapy | Original Article

Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner

Authors: Simone Silva dos Santos, Helenita Antonia de Oliveira, Ednei Luiz Antonio, Ighor Luiz Azevedo Teixeira, Barbara Sampaio Dias Martins Mansano, Flávio André Silva, Paulo Tarso Camillo de Carvalho, Paulo José Ferreira Tucci, Andrey Jorge Serra

Published in: Lasers in Medical Science | Issue 8/2020

Abstract

High-intensity resistance exercise (RE) increases oxidative stress leading to deleterious effects on muscle performance and recovery. The aim of this study was to assess the effect of applying low-level laser therapy (LLLT) prior to a RE session on muscle oxidative stress and to determine the possible influence of the dosimetric parameters. Female Wistar rats were assigned to non-LLLT (Ctr: non-exercised control; RNI: RE) or LLLT groups subjected to RE (radiant energy: 4 J, 8 J, and 12 J, respectively). RE consisted of four maximum load climbs. An 830-nm DMC Lase Photon III was used to irradiate three points in gastrocnemius muscles (two limbs) before exercise. Animals were euthanized after 60 min after the end of the exercise, and muscle tissue was removed for analysis of oxidative stress markers. All doses resulted in the prevention of increased lipoperoxidation; however, LLLT prevented protein oxidation only in rats that were pretreated with 8 J and 12 J of energy by LLLT. RE and LLLT did not change catalase activity. However, RE resulted in lower superoxide dismutase activity, and the opposite was observed in the LLLT group. These data indicate that LLLT prior to RE can prevent muscle oxidative stress. This study is the first to evaluate the impact of dosimetric LLLT parameters on the oxidative stress induced by RE, wherein both 8 J and 12 J of energy afforded significant protection.

Retamoso LT, Silveira MEJ, Lima FD, Busanello GL, Bresciani G, Ribeiro LR, Chagas PM, Nogueira CW, Braga AC, Furian AF, Oliveira MS, Fighera MR, Royes LF (2016) Increased xanthine oxidase-related ROS production and TRPV1 synthesis preceding DOMS post-eccentric exercise in rats. Life Sci 152:52–59. https://doi.org/10.1016/j.lfs.2016.03.029CrossRefPubMed

Wiecek M, Maciejczyk M, Szymura J, Szygula Z (2017) Effect of maximal-intensity exercise on systemic nitro-oxidative stress in men and women. Redox Rep 22(4):176–182. https://doi.org/10.1080/13510002.2016.1169622CrossRefPubMed

Sunemi SM, Silva FA, Antonio EL, Tucci PJF, Serra AJ (2019) Photobiomodulation: newly discovered actions in resistance exercise. Reactive oxygen species 7(21):148–153. https://doi.org/10.20455/ros.2019.829CrossRef

De Marchi T, Leal Junior EC, Bortoli C, Tomazoni SS, Lopes-Martins RA, Salvador M (2012) Low-level laser therapy (LLLT) in human progressive-intensity running: effects on exercise performance, skeletal muscle status, and oxidative stress. Lasers Med Sci 27(1):231–236. https://doi.org/10.1007/s10103-011-0955-5CrossRefPubMed

De Marchi T, Leal-Junior ECP, Lando KC, Cimadon F, Vanin AA, da Rosa DP, Salvador M (2019) Photobiomodulation therapy before futsal matches improves the staying time of athletes in the court and accelerates post-exercise recovery. Lasers Med Sci 34(1):139–148. https://doi.org/10.1007/s10103-018-2643-1CrossRefPubMed

de Oliveira HA, Antonio EL, Arsa G, Santana ET, Silva FA, Junior DA, Dos Santos S, de Carvalho PTC, Leal-Junior ECP, Araujo A, De Angelis K, Bocalini DS, Junior JAS, Tucci PJF, Serra AJ (2018) Photobiomodulation leads to reduced oxidative stress in rats submitted to high-intensity resistive exercise. Oxidative Med Cell Longev 2018:5763256. https://doi.org/10.1155/2018/5763256CrossRef

Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy - an update. Dose-Response 9(4):602–618. https://doi.org/10.2203/dose-response.11-009.HamblinCrossRefPubMedPubMedCentral

de Oliveira HA, Antonio EL, Silva FA, de Carvalho PTC, Feliciano R, Yoshizaki A, Vieira SS, de Melo BL, Leal-Junior ECP, Labat R, Bocalini DS, Silva Junior JA, Tucci PJF, Serra AJ (2018) Protective effects of photobiomodulation against resistance exercise-induced muscle damage and inflammation in rats. J Sports Sci 36(20):2349–2357. https://doi.org/10.1080/02640414.2018.1457419CrossRefPubMed

Antonialli FC, De Marchi T, Tomazoni SS, Vanin AA, dos Santos GV, de Paiva PR, Pinto HD, Miranda EF, de Tarso Camillo de Carvalho P, Leal-Junior EC (2014) Phototherapy in skeletal muscle performance and recovery after exercise: effect of combination of super-pulsed laser and light-emitting diodes. Lasers Med Sci 29(6):1967–1976. https://doi.org/10.1007/s10103-014-1611-7CrossRefPubMed

10.

de Oliveira AR, Vanin AA, Tomazoni SS, Miranda EF, Albuquerque-Pontes GM, De Marchi T, Dos Santos GV, de Paiva PRV, Imperatori TBG, de Carvalho PTC, Bjordal JM, Leal-Junior ECP (2017) Pre-exercise infrared Photobiomodulation therapy (810 nm) in skeletal muscle performance and postexercise recovery in humans: what is the optimal power output? Photomed Laser Surg 35(11):595–603. https://doi.org/10.1089/pho.2017.4343CrossRefPubMed

11.

Grandinetti V, Carlos FP, Antonio EL, de Oliveira HA, Dos Santos LFN, Yoshizaki A, Mansano B, Silva FA, Porte LA, Albuquerque-Pontes GM, de Carvalho PTC, Manchini MT, Leal-Junior EC, Tucci PJF, Serra AJ (2019) Photobiomodulation therapy combined with carvedilol attenuates post-infarction heart failure by suppressing excessive inflammation and oxidative stress in rats. Sci Rep 9(1):9425. https://doi.org/10.1038/s41598-019-46021-1CrossRefPubMedPubMedCentral

12.

Cohen J (1992) A power primer. Psychol Bull 112(1):155–159CrossRef

13.

Amadio EM, Serra AJ, Guaraldo SA, Silva JA Jr, Antonio EL, Silva F, Portes LA, Tucci PJ, Leal-Junior EC, de Carvalho PT (2015) The action of pre-exercise low-level laser therapy (LLLT) on the expression of IL-6 and TNF-alpha proteins and on the functional fitness of elderly rats subjected to aerobic training. Lasers Med Sci 30(3):1127–1134. https://doi.org/10.1007/s10103-015-1713-xCrossRefPubMed

14.

De Marchi T, Schmitt VM, Machado GP, de Sene JS, de Col CD, Tairova O, Salvador M, Leal-Junior EC (2017) Does photobiomodulation therapy is better than cryotherapy in muscle recovery after a high-intensity exercise? A randomized, double-blind, placebo-controlled clinical trial. Lasers Med Sci 32(2):429–437. https://doi.org/10.1007/s10103-016-2139-9CrossRefPubMed

15.

Nampo FK, Cavalheri V, Dos Santos SF, de Paula RS, Camargo EA (2016) Low-level phototherapy to improve exercise capacity and muscle performance: a systematic review and meta-analysis. Lasers Med Sci 31(9):1957–1970. https://doi.org/10.1007/s10103-016-1977-9CrossRefPubMed

16.

Baroni BM, Leal Junior EC, De Marchi T, Lopes AL, Salvador M, Vaz MA (2010) Low level laser therapy before eccentric exercise reduces muscle damage markers in humans. Eur J Appl Physiol 110(4):789–796. https://doi.org/10.1007/s00421-010-1562-zCrossRefPubMed

17.

Ferraresi C, de Sousa MV, Huang YY, Bagnato VS, Parizotto NA, Hamblin MR (2015) Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice. Lasers Med Sci 30(4):1259–1267. https://doi.org/10.1007/s10103-015-1723-8CrossRefPubMed

18.

d'Ischia M, Palumbo A, Buzzo F (2000) Interactions of nitric oxide with lipid peroxidation products under aerobic conditions: inhibitory effects on the formation of malondialdehyde and related thiobarbituric acid-reactive substances. Nitric Oxide 4(1):4–14. https://doi.org/10.1006/niox.1999.0268CrossRefPubMed

19.

Moselhy HF, Reid RG, Yousef S, Boyle SP (2013) A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC. J Lipid Res 54(3):852–858. https://doi.org/10.1194/jlr.D032698CrossRefPubMedPubMedCentral

20.

Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K (2002) Methods for testing antioxidant activity. Analyst 127(1):183–198CrossRef

21.

Dunlop RA, Brunk UT, Rodgers KJ (2009) Oxidized proteins: mechanisms of removal and consequences of accumulation. IUBMB Life 61(5):522–527. https://doi.org/10.1002/iub.189CrossRefPubMed

22.

Radak Z, Zhao Z, Koltai E, Ohno H, Atalay M (2013) Oxygen consumption and usage during physical exercise: the balance between oxidative stress and ROS-dependent adaptive signaling. Antioxid Redox Signal 18(10):1208–1246. https://doi.org/10.1089/ars.2011.4498CrossRefPubMedPubMedCentral

23.

Kozakowska M, Pietraszek-Gremplewicz K, Jozkowicz A, Dulak J (2015) The role of oxidative stress in skeletal muscle injury and regeneration: focus on antioxidant enzymes. J Muscle Res Cell Motil 36(6):377–393. https://doi.org/10.1007/s10974-015-9438-9CrossRefPubMed

24.

Morrison D, Hughes J, Della Gatta PA, Mason S, Lamon S, Russell AP, Wadley GD (2015) Vitamin C and E supplementation prevents some of the cellular adaptations to endurance-training in humans. Free Radic Biol Med 89:852–862. https://doi.org/10.1016/j.freeradbiomed.2015.10.412CrossRefPubMed

Title: Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner
Authors: Simone Silva dos Santos
Helenita Antonia de Oliveira
Ednei Luiz Antonio
Ighor Luiz Azevedo Teixeira
Barbara Sampaio Dias Martins Mansano
Flávio André Silva
Paulo Tarso Camillo de Carvalho
Paulo José Ferreira Tucci
Andrey Jorge Serra
Publication date: 01-10-2020
Publisher: Springer London
Keywords: Phototherapy
Laser
Published in: Lasers in Medical Science / Issue 8/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-02951-1

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Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner

Abstract

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Abstract

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