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01-10-2020 | Diode Laser | Original Article

Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway

Authors: Jie Feng, Xiaoyan Li, Siying Zhu, Yongmei Xie, Juan Du, Huabing Ge, Yuxing Bai, Yi Liu, Lijia Guo

Published in: Lasers in Medical Science | Issue 8/2020

Abstract

Photobiomodulation (PBM) has been shown to improve wound healing by promoting mesenchymal stem cell migration and proliferation. However, it remains unknown whether an 808-nm diode laser can influence human gingival mesenchymal stem cells (HGMSCs), and which dose this works well. In the present study, it was found that PBM could promote the migration of HGMSCs but not the proliferation. Furthermore, PBM could activate mitochondrial ROS, which could elevate the phosphorylation levels of JNK and IKB in HGMSCs, and further activate NF-κB as the nuclear translocation of p65 is elevated. Taken together, these present results indicate that PBM might promote cell migration via the ROS/JNK/NF-κB pathway.

Franz RW, Parks A, Shah KJ, Hankins T, Hartman JF, Wright ML (2009) Use of autologous bone marrow mononuclear cell implantation therapy as a limb salvage procedure in patients with severe peripheral arterial disease. J Vasc Surg 50:1378–1390CrossRef

Mester E, Szende B, Gartner P (1968) The effect of laser beams on the growth of hair in mice. Radiobiol Radiother 9(5):621–626

Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR (2012) The nuts and bolts of low-level laser (light) therapy. Ann Biomed Eng 40(2):516–533. https://doi.org/10.1007/s10439-011-0454-7CrossRefPubMed

Hopkins JT, McLoda TA, Seegmiller JG, David Baxter G (2004) Low-level laser therapy facilitates superficial wound healing in humans: a triple-blind, sham-controlled study. J Athl Train 39(3):223–229PubMedPubMedCentral

Almeida-Lopes L, Rigau J, Zangaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29(2):179–184CrossRef

Chow RT, Heller GZ, Barnsley L (2006) The effect of 300 mW, 830 nm laser on chronic neck pain: a double-blind, randomized, placebo-controlled study. Pain 124(1–2):201–210. https://doi.org/10.1016/j.pain.2006.05.018CrossRefPubMed

Mester E, Juhasz J, Varga P, Karika G (1968) Lasers in clinical practice. Acta Chir Acad Sci Hung 9(3):349–357PubMed

Kreisler M, Christoffers AB, Willershausen B, d'Hoedt B (2003) Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: an in vitro study. J Clin Periodontol 30(4):353–358. https://doi.org/10.1034/j.1600-051x.2003.00001.xCrossRefPubMed

Medrado AR, Pugliese LS, Reis SR, Andrade ZA (2003) Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers Surg Med 32(3):239–244. https://doi.org/10.1002/lsm.10126CrossRefPubMed

10.

Yu W, Naim JO, Lanzafame RJ (1997) Effects of photostimulation on wound healing in diabetic mice. Lasers Surg Med 20(1):56–63CrossRef

11.

Kim YD, Kim SS, Kim SJ, Kwon DW, Jeon ES, Son WS (2010) Low-level laser irradiation facilitates fibronectin and collagen type I turnover during tooth movement in rats. Lasers Med Sci 25(1):25–31. https://doi.org/10.1007/s10103-008-0585-8CrossRefPubMed

12.

Liao X, Xie GH, Liu HW, Cheng B, Li SH, Xie S, Xiao LL, Fu XB (2014) Helium-neon laser irradiation promotes the proliferation and migration of human epidermal stem cells in vitro: proposed mechanism for enhanced wound re-epithelialization. Photomed Laser Surg 32(4):219–225. https://doi.org/10.1089/pho.2013.3667CrossRefPubMedPubMedCentral

13.

Tsai WC, Hsu CC, Pang JH, Lin MS, Chen YH, Liang FC (2012) Low-level laser irradiation stimulates tenocyte migration with up-regulation of dynamin II expression. PLoS One 7(5):e38235. https://doi.org/10.1371/journal.pone.0038235CrossRefPubMedPubMedCentral

14.

Schindl A, Schindl M, Pernerstorfer-Schon H, Schindl L (2000) Low-intensity laser therapy: a review. J Investig Med 48(5):312–326PubMed

15.

Esmaeelinejad M, Bayat M, Darbandi H, Bayat M, Mosaffa N (2014) The effects of low-level laser irradiation on cellular viability and proliferation of human skin fibroblasts cultured in high glucose mediums. Lasers Med Sci 29(1):121–129. https://doi.org/10.1007/s10103-013-1289-2CrossRefPubMed

16.

Fushimi T, Inui S, Nakajima T, Ogasawara M, Hosokawa K, Itami S (2012) Green light emitting diodes accelerate wound healing: characterization of the effect and its molecular basis in vitro and in vivo. Wound Repair Regen 20(2):226–235. https://doi.org/10.1111/j.1524-475X.2012.00771.xCrossRefPubMed

17.

Azevedo LH, de Paula EF, Moreira MS, de Paula EC, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth: a pilot study. Lasers Med Sci 21(2):86–89. https://doi.org/10.1007/s10103-006-0379-9CrossRefPubMed

18.

Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med 36(1):8–12. https://doi.org/10.1002/lsm.20117CrossRefPubMed

19.

Pourzarandian A, Watanabe H, Ruwanpura SM, Aoki A, Ishikawa I (2005) Effect of low-level Er:YAG laser irradiation on cultured human gingival fibroblasts. J Periodontol 76(2):187–193. https://doi.org/10.1902/jop.2005.76.2.187CrossRefPubMed

20.

Chen AC, Arany PR, Huang YY, Tomkinson EM, Sharma SK, Kharkwal GB, Saleem T, Mooney D, Yull FE, Blackwell TS, Hamblin MR (2011) Low-level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS One 6(7):e22453. https://doi.org/10.1371/journal.pone.0022453CrossRefPubMedPubMedCentral

21.

Ejiri K, Aoki A, Yamaguchi Y, Ohshima M, Izumi Y (2014) High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells. Lasers Med Sci 29(4):1339–1347. https://doi.org/10.1007/s10103-013-1292-7CrossRefPubMed

22.

Shingyochi Y, Kanazawa S, Tajima S, Tanaka R, Mizuno H, Tobita M (2017) A low-level carbon dioxide laser promotes fibroblast proliferation and migration through activation of Akt, ERK, and JNK. PLoS One 12(1):e0168937. https://doi.org/10.1371/journal.pone.0168937CrossRefPubMedPubMedCentral

23.

West AP, Brodsky IE, Rahner C, Woo DK, Erdjument-Bromage H, Tempst P, Walsh MC, Choi Y, Shadel GS, Ghosh S (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472(7344):476–480. https://doi.org/10.1038/nature09973CrossRefPubMedPubMedCentral

24.

Mailloux RJ, Harper ME (2011) Uncoupling proteins and the control of mitochondrial reactive oxygen species production. Free Radic Biol Med 51(6):1106–1115. https://doi.org/10.1016/j.freeradbiomed.2011.06.022CrossRefPubMed

25.

Bulua AC, Simon A, Maddipati R, Pelletier M, Park H, Kim KY, Sack MN, Kastner DL, Siegel RM (2011) Mitochondrial reactive oxygen species promote production of proinflammatory cytokines and are elevated in TNFR1-associated periodic syndrome (TRAPS). J Exp Med 208(3):519–533. https://doi.org/10.1084/jem.20102049CrossRefPubMedPubMedCentral

26.

Bogdan C, Rollinghoff M, Diefenbach A (2000) Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity. Curr Opin Immunol 12(1):64–76CrossRef

27.

Murphy MP, Smith RA (2007) Targeting antioxidants to mitochondria by conjugation to lipophilic cations. Annu Rev Pharmacol Toxicol 47:629–656. https://doi.org/10.1146/annurev.pharmtox.47.120505.105110CrossRefPubMed

28.

Emre Y, Hurtaud C, Nubel T, Criscuolo F, Ricquier D, Cassard-Doulcier AM (2007) Mitochondria contribute to LPS-induced MAPK activation via uncoupling protein UCP2 in macrophages. Biochem J 402(2):271–278. https://doi.org/10.1042/bj20061430CrossRefPubMedPubMedCentral

29.

Kim DY, Jun JH, Lee HL, Woo KM, Ryoo HM, Kim GS, Baek JH, Han SB (2007) N-acetylcysteine prevents LPS-induced pro-inflammatory cytokines and MMP2 production in gingival fibroblasts. Arch Pharm Res 30(10):1283–1292CrossRef

30.

Ho IA, Yulyana Y, Sia KC, Newman JP, Guo CM, Hui KM, Lam PY (2014) Matrix metalloproteinase-1-mediated mesenchymal stem cell tumor tropism is dependent on crosstalk with stromal derived growth factor 1/C-X-C chemokine receptor 4 axis. FASEB J 28(10):4359–4368. https://doi.org/10.1096/fj.14-252551CrossRefPubMed

31.

Wang W, Pan H, Murray K, Jefferson BS, Li Y (2009) Matrix metalloproteinase-1 promotes muscle cell migration and differentiation. Am J Pathol 174(2):541–549. https://doi.org/10.2353/ajpath.2009.080509CrossRefPubMedPubMedCentral

Title: Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway
Authors: Jie Feng
Xiaoyan Li
Siying Zhu
Yongmei Xie
Juan Du
Huabing Ge
Yuxing Bai
Yi Liu
Lijia Guo
Publication date: 01-10-2020
Publisher: Springer London
Keywords: Diode Laser
Laser
Published in: Lasers in Medical Science / Issue 8/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03040-z

At a glance: The STEP trials

Springer Medicine

Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS/JNK/NF-κB/MMP-1 pathway

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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