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Brain Tumor Pathology
Published in: Brain Tumor Pathology 4/2017

01-10-2017 | Original Article

Radiation-induced gliomas: a report of four cases and analysis of molecular biomarkers

Authors: Tsunehito Nakao, Yasuo Sasagawa, Sumihito Nobusawa, Yasushi Takabatake, Hemragul Sabit, Masashi Kinoshita, Katsuyoshi Miyashita, Yasuhiko Hayashi, Hideaki Yokoo, Mitsutoshi Nakada

Published in: Brain Tumor Pathology | Issue 4/2017

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Abstract

Radiation-induced glioma (RIG) is a rare secondary glioma. The tumors morphologically resemble their sporadically arising counterparts. Recently, the WHO classification of tumors of the central nervous system was revised to incorporate molecular biomarkers together with classic histological features. The status of molecular biomarkers in RIG, however, remains unclear. The objective of this study was to investigate if commonly accepted glioma-specific biomarkers are relevant in RIGs. Among 269 gliomas diagnosed as WHO grade 2, 3 and 4 in our institution, four were diagnosed as RIGs. Immunohistochemical (IHC) staining for isocitrate dehydrogenase 1 (IDH1), p53, alpha thalassemia/mental retardation syndrome X-linked (ATRX), and H3K27M, and direct DNA sequencing of IDH1/2, telomerase reverse transcriptase (TERT) promoter, Histone H3.3 (H3F3A) and B-Raf (BRAF) genes was performed. All tumor specimens were IDH1-, p53- and H3K27M-negative. The nuclei of tumor cells in all cases exhibited positive staining for ATRX. In direct DNA sequencing analysis, no IDH1, IDH2, TERT promoter, H3F3A or BRAF mutations were found in any of the cases. Our findings suggest that these characteristic glioma-associated molecular mutations may be rare events in RIGs. More RIGs need to be tested for analysis of molecular biomarkers to clarify the clinical and histopathological spectra of this tumor.
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Metadata
Title
Radiation-induced gliomas: a report of four cases and analysis of molecular biomarkers
Authors
Tsunehito Nakao
Yasuo Sasagawa
Sumihito Nobusawa
Yasushi Takabatake
Hemragul Sabit
Masashi Kinoshita
Katsuyoshi Miyashita
Yasuhiko Hayashi
Hideaki Yokoo
Mitsutoshi Nakada
Publication date
01-10-2017
Publisher
Springer Singapore
Published in
Brain Tumor Pathology / Issue 4/2017
Print ISSN: 1433-7398
Electronic ISSN: 1861-387X
DOI
https://doi.org/10.1007/s10014-017-0292-x

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