Top

Published in:

01-07-2020 | Encephalitis | Annotated Sequence Record

Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes

Authors: Susana L. Padilla, Karla Prieto, David J. Dohm, Michael J. Turell, Terry A. Klein, Roberto Fernández, Douglas M. Watts, Robert G. Lowen, Gustavo F. Palacios, Margaret L. Pitt, Michael R. Wiley, Farooq Nasar

Published in: Archives of Virology | Issue 7/2020

Abstract

Venezuelan equine encephalitis virus (VEEV) is an important pathogen of medical and veterinary importance in the Americas. In this report, we present the complete genome sequences of five VEEV isolates obtained from pools of Culex (Melanoconion) gnomatos (4) or Culex (Melanoconion) pedroi (1) from Iquitos, Peru. Genetic and phylogenetic analyses showed that all five isolates grouped within the VEEV complex sister to VEEV IIIC and are members of subtype IIID. This is the first report of full-length genomic sequences of VEEV IIID.

Griffin DE (2013) Alphaviruses. In: Fields BN, Knipe DM, Howley PM (eds) Fields virology, 6th edn. Wolters Kluwer Health/Lippincott Williams & Wilkins, Philadelphia, PA, pp 1023–1068

Nasar F, Palacios G, Gorchakov RV, Guzman H, Da Rosa AP, Savji N, Popov VL, Sherman MB, Lipkin WI, Tesh RB, Weaver SC (2012) Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication. Proc Natl Acad Sci USA 109:14622–14627CrossRef

Weaver SC, Ferro C, Barrera R, Boshell J, Navarro JC (2004) Venezuelan equine encephalitis. Annu Rev Entomol 49:141–174CrossRef

Aguilar PV, Greene IP, Coffey LL, Medina G, Moncayo AC, Anishchenko M, Ludwig GV, Turell MJ, O’Guinn ML, Lee J, Tesh RB, Watts DM, Russell KL, Hice C, Yanoviak S, Morrison AC, Klein TA, Dohm DJ, Guzman H, Travassos da Rosa AP, Guevara C, Kochel T, Olson J, Cabezas C, Weaver SC (2004) Endemic Venezuelan equine encephalitis in northern Peru. Emerg Infect Dis 10:880–888CrossRef

Turell MJ, O’Guinn ML, Jones JW, Sardelis MR, Dohm DJ, Watts DM, Fernandez R, Travassos da Rosa A, Guzman H, Tesh R, Rossi CA, Ludwig V, Mangiafico JA, Kondig J, Wasieloski LP Jr, Pecor J, Zyzak M, Schoeler G, Mores CN, Calampa C, Lee JS, Klein TA (2005) Isolation of viruses from mosquitoes (Diptera: Culicidae) collected in the Amazon Basin region of Peru. J Med Entomol 42:891–898CrossRef

Djikeng A, Halpin R, Kuzmickas R, Depasse J, Feldblyum J, Sengamalay N, Afonso C, Zhang X, Anderson NG, Ghedin E, Spiro DJ (2008) Viral genome sequencing by random priming methods. BMC Genom 9:5CrossRef

Leguia M, Loyola S, Rios J, Juarez D, Guevara C, Silva M, Prieto K, Wiley M, Kasper MR, Palacios G, Bausch DG (2015) Full genomic characterization of a saffold virus isolated in Peru. Pathogens 4:816–825CrossRef

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410CrossRef

Boisvert S, Raymond F, Godzaridis E, Laviolette F, Corbeil J (2012) Ray Meta: scalable de novo metagenome assembly and profiling. Genome Biol 13:R122CrossRef

10.

Schmieder R, Edwards R (2011) Quality control and preprocessing of metagenomic datasets. Bioinformatics 27:863–864CrossRef

11.

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797CrossRef

12.

Gouy M, Guindon S, Gascuel O (2010) SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol Biol Evol 27:221–224CrossRef

13.

Felsenstein J (1989) PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166

Title: Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes
Authors: Susana L. Padilla
Karla Prieto
David J. Dohm
Michael J. Turell
Terry A. Klein
Roberto Fernández
Douglas M. Watts
Robert G. Lowen
Gustavo F. Palacios
Margaret L. Pitt
Michael R. Wiley
Farooq Nasar
Publication date: 01-07-2020
Publisher: Springer Vienna
Keyword: Encephalitis
Published in: Archives of Virology / Issue 7/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04647-x

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 7/2020

Complete genome sequence of a novel grapevine-infecting member of the genus Polerovirus, grapevine polerovirus 1

Development of a sandwich ELISA for the detection of Chinese sacbrood virus infection

Isolation and identification of two new strains of mammalian orthoreovirus from Chinese tree shrews

Generation of recombinant VP3 protein of infectious bursal disease virus in three different expression systems, antigenic analysis of the obtained polypeptides and development of an ELISA test

Camellia ringspot-associated virus 4, a proposed new foveavirus from Camellia japonica

Full-genome sequences of GII.13[P21] recombinant norovirus strains from an outbreak in Changsha, China

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page