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Published in:

01-08-2018 | Original Article

Different susceptibility to porcine reproductive and respiratory syndrome virus infection among Chinese native pig breeds

Authors: Chunhua Meng, Lei Su, Yinxia Li, Qianming Zhu, Jingxin Li, Huili Wang, Qiang He, Chunling Wang, Wei Wang, Shaoxian Cao

Published in: Archives of Virology | Issue 8/2018

Abstract

China is rich in native pig breeds, yet information regarding the susceptibility/resistance of local breeds to porcine reproductive and respiratory syndrome virus (PRRSV) infection is lacking. In the present study, an in vitro method based on assessing PRRSV replication in porcine alveolar macrophages (PAMs) was established to evaluate PRRSV susceptibility/resistance in a commercial pig breed (Landrace) and five native pig breeds from Jiangsu and Anhui provinces in China. Expression levels of cytokines (IL-8, IL-10, TNF-α and IFN-γ), Toll-like receptor 3 (TLR3), CD163 (PRRSV receptor), and sialoadhesin (Sn, PRRSV receptor) in infected pigs were determined using real-time PCR, and the association between PRRSV susceptibility/resistance and the abundance of the cytokines and receptors was investigated. The viral replication rate and titer at 0, 6, 12 18, 24 and 36 hours postinfection (hpi) were determined to assess the proliferation dynamics of PRRSV NJGC in PAMs. Based on the PRRSV proliferation dynamics, the results indicated that Dingyuan pigs were the most susceptible to PRRSV infection, whereas Jiangquhai pigs were the least susceptible to PRRSV infection among the six pig breeds tested, as indicated by measuring PRRSV replication and the viral load in PAMs. The different levels of susceptibility to PRRSV infection in PAMs may be associated with differences in the abundance of CD163 (PRRSV receptor), cytokines IL-8, IFN-γ, and TNF-α in Jiangquhai and Dingyuan pig breeds after viral inoculation.

Xiao S, Mo D, Wang Q, Jia J, Qin L, Yu X, Niu Y, Zhao X, Liu X, Chen Y (2010) Aberrant host immune response induced by highly virulent PRRSV identified by digital gene expression tag profiling. BMC Genom 11(1):544CrossRef

Zhang J (2016) Coinfection with type 1 and type 2 PRRSV. Vet Rec 178:288–290CrossRefPubMed

Zhang HF, Kono H, Kubota S (2014) An integrated epidemiological and economic analysis of vaccination against highly pathogenic porcine reproductive and respiratory syndrome (PRRS) in Thua Thien Hue Province, Vietnam. Asian-Australasian. J Anim Sci 27:1499–1512

Lunney JK, Fang Y, Ladinig A, Chen N, Li Y, Rowland B, Renukaradhya GJ (2016) Porcine reproductive and respiratory syndrome virus (PRRSV): pathogenesis and interaction with the immune system. Annu Rev Anim Biosci 4:129–154CrossRefPubMed

Zhang Q, Yoo D (2015) PRRS virus receptors and their role for pathogenesis. Vet Microbiol 177:229–241CrossRefPubMed

Zhu L, Song H, Zhang X, Xia X, Sun H (2014) Inhibition of porcine reproductive and respiratory syndrome virusinfection by recombinant adenovirus- and/or exosome-delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors. Virol J 11:225CrossRefPubMedCentralPubMed

Wang F, Qiu H, Zhang Q, Peng Z, Liu B (2012) Association of two porcine reproductive and respiratory syndrome virus(PRRSV) receptor genes, CD163 and SN with immune traits. Mol Biol Rep 39(4):3971–3976CrossRefPubMed

Prather RS, Whitworth KM, Schommer SK, Wells KD (2017) Genetic engineering alveolar macrophages for host resistance to PRRSV. Vet Microbiol 209:124–129CrossRefPubMed

Yang H, Zhang J, Zhang X, Shi J, Pan Y, Zhou R, Li G, Li Z, Cai G, Wu Z (2018) CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus. Antiviral Res 151:63–70CrossRefPubMed

10.

Suradhat S, Thanawongnuwech R (2003) Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus. J Gen Virol 84:2755–2760CrossRefPubMed

11.

Ferrarini G, Borghetti P, De Angelis E, Ferrari L, Canelli E, Catella A, De Lecce R, Martelli P (2015) Immunoregulatory signal FoxP3, cytokine gene expression and IFN-γ cell responsiveness upon porcine reproductive and respiratory syndrome virus (PRRSV) natural infection. Res Vet Sci 103:96–102CrossRefPubMed

12.

Petry DB, Lunney J, Boyd P, Kuhar D, Blankenship E, Johnson RK (2007) Differential immunity in pigs with high and low responses to porcine reproductive and respiratory syndrome virus infection. J Anim Sci 85:2075–2092CrossRefPubMed

13.

Zhang LL, Liu J, Bai J, Wang XY, Li YF, Jiang P (2013) Comparative expression of toll-like receptors and inflammatory cytokines in pigs infected with different virulent porcine reproductive and respiratory syndrome virus isolates. Virol J 10:135CrossRefPubMedCentralPubMed

14.

Jiang C, Xing F, Xing J, Jiang Y, Zhou E (2013) Different expression patterns of PRRSV mediator genes in the lung tissues of PRRSV resistant and susceptible pigs. Dev Comp Immunol 39:127–131CrossRefPubMed

15.

Wang C, Wu B, Amer S, Luo J, Zhang HM, Guo YH, Dong GY, Zhao BH, He HX (2010) Phylogenetic analysis and molecular characteristic of seven variant Chinese field isolates of PRRSV. BMC Microbiol 10:1–11CrossRef

16.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2^−ΔΔCt method. Methods 25:402–408CrossRef

17.

Vincent AL, Thacker BJ, Halbur PG, Rothschild MF, Thacker EL (2006) An investigation of susceptibility to porcine reproductive and respiratory syndrome virusbetween two genetically diverse commercial lines of pigs. J Anim Sci 84:49–57CrossRefPubMed

18.

Ait-Ali T, Wilson AD, Wescott DG (2007) Innate immune response to replication of porcine reproductive and respiratory syndrome virus in isolated porcine alveolar macrophages. Viral Immunol 20:105–118CrossRefPubMed

19.

Xing J, Xing F, Zhang C, Zhang YJ, Wang N, Li YP, Yang LJ, Jiang CL, Zhang CY, Wen CH, Jiang YL (2014) Genome-wide gene expression profiles in lung tissues of pig breeds differing in resistance to porcine reproductive and respiratory syndrome virus. PLoS One 9(1):e86101CrossRefPubMedCentralPubMed

20.

Vanderheijden N, Delputte PL, Favoreel HW, Vandekerckhove J, Damme JV, van Woensel PA, Nauwynck HJ (2003) Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages. J Virol 77:8207–8215CrossRefPubMedCentralPubMed

21.

Van Gorp H, Van Breedam W, Delputte PL, Nauwynck HJ (2008) Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus. J Gen Virol 89:2943–2953CrossRefPubMed

22.

Liu YG, Shi WD, Zhou EM, Wang SJ, Hu SP, Cai XH, Rong FL, Wu JB, Xu M, Xu MM, Li LQ (2010) Dynamic changes in inflammatory cytokines in pigs infected with highly pathogenic porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol 19:1439–1445CrossRef

23.

24.

Genini S, Delputte PL, Malinverni R, Cecere M, Stella A, Nauwynck HJ, Giuffra E (2008) Genome-wide transcriptional response of primary alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus. J Gen Virol 89:2550–2564CrossRefPubMedCentralPubMed

25.

Lunney JK, Fritz ER, Reecy JM, Kuhar D, Prucnal E, Molina R, Christopher-Hennings J, Zimmerman J, Rowland RR (2010) Interleukin-8, interleukin-1β, and interferon-γ levels are linked to PRRS virus clearance. Viral Immunol 23:127–134CrossRefPubMed

26.

Gómez-Laguna J, Salguero FJ, Barranco I, Pallarés FJ, Rodríguez-Gómez IM, Bernabé A, Carrasco L (2010) Cytokine expression by macrophages in the lung of pigs infected with the porcine reproductive and respiratory syndrome virus. J Comp Pathol 142:51–60CrossRefPubMed

27.

Sang Y, Rowland RRR, Blecha F (2011) Interaction between innate immunity and porcine reproductive and respiratory syndrome virus. Anim Health Res Rev 12:149–167CrossRefPubMed

28.

Jung K, Renukaradhya GJ, Alekseev KP, Fang Y, Tang Y, Saif LJ (2009) Porcine reproductive and respiratory syndrome virus modifies innate immunity and alters disease outcome in pigs subsequently infected with porcine respiratory coronavirus: implications for respiratory viral co-infections. J Gen Virol 90:2713–2723CrossRefPubMedCentralPubMed

29.

Wang L, Hu S, Liu Q, Li Y, Xu L, Zhang Z, Cai X, He X (2017) Porcine alveolar macrophage polarization is involved in inhibition of porcine reproductive and respiratory syndrome virus (PRRSV) replication. J Vet Med Sci 79(11):1906–1915CrossRefPubMedCentralPubMed

Title: Different susceptibility to porcine reproductive and respiratory syndrome virus infection among Chinese native pig breeds
Authors: Chunhua Meng
Lei Su
Yinxia Li
Qianming Zhu
Jingxin Li
Huili Wang
Qiang He
Chunling Wang
Wei Wang
Shaoxian Cao
Publication date: 01-08-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 8/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3821-y

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