Top

Published in:

Open Access 01-03-2018 | Brief Report

New virus of the family Flaviviridae detected in lumpfish (Cyclopterus lumpus)

Authors: Renate Hvidsten Skoge, Jarle Brattespe, Arnfinn Lodden Økland, Heidrun Plarre, Are Nylund

Published in: Archives of Virology | Issue 3/2018

Abstract

In this study, we determined the complete coding sequence of a putative new member of the family Flaviviridae, named “Cyclopterus lumpus virus” (CLuV), which is associated with a serious disease in lumpfish (Cyclopterus lumpus). The virus was present in all tissues tested, but pathology was primarily observed in the liver and kidneys. CLuV shows low but distinct similarity to the unassigned Tamana bat virus (TABV). Unlike other known members of the family Flaviviridae, translation of the entire CLuV polyprotein is dependent on a − 1 ribosomal frameshift in the NS2A region.

Tørrissen O, Jones S, Asche F, Guttormsen A, Skilbrei OT, Nilsen F, Horsberg TE, Jackson D (2013) Salmon lice–impact on wild salmonids and salmon aquaculture. J Fish Dis 36(3):171–194. https://doi.org/10.1111/jfd.12061 CrossRefPubMedPubMedCentral

Heuch PABP, Finstad B, Holst JC, Asplin L, Nilsen F (2005) A review of the Norwegian “National action plan against salmon lice on salmonids”: the effect on wild salmonids. Aquaculture 246:79–92CrossRef

Leclerq E, Davie A, Migaud H (2013) Delousing efficiency of farmed ballan wrasse (Labrus bergylta) against Lepeophtheirus salmonis infecting Atlantic salmon (Salmo salar) post-smolts. Pest Manag Sci 70:1274–1282CrossRef

Imsland AK, Reynolds P, Eliassen G, Hangstad TA, Foss A, Vikingstad E, Elvegård TA (2014) The use of lumpfish (Cyclopterus lumpus L.) to control sea lice (Lepeophtheirus salmonis Krøyer) infestations in intensively farmed Atlantic salmon (Salmo salar L.). Aquaculture 424–425:18–23CrossRef

Alarcon M, Gulla S, Rosaeg MV, Ronneseth A, Wergeland H, Poppe TT, Nilsen H, Colquhoun DJ (2016) Pasteurellosis in lumpsucker Cyclopterus lumpus, farmed in Norway. J Fish Dis 39(4):489–495. https://doi.org/10.1111/jfd.12366 CrossRefPubMed

Alarcon M, Thoen E, Poppe TT, Borno G, Mohammad SN, Hansen H (2016) Co-infection of Nucleospora cyclopteri (Microsporidia) and Kudoa islandica (Myxozoa) in farmed lumpfish, Cyclopterus lumpus L., in Norway: a case report. J Fish Dis 39(4):411–418. https://doi.org/10.1111/jfd.12372 CrossRefPubMed

Cavin JM, Donahoe SL, Frasca S Jr, Innis CJ, Kinsel MJ, Kurobe T, Naples LM, Nyaoke A, Poll CP, Weber EP 3rd (2012) Myxobolus albi infection in cartilage of captive lumpfish (Cyclopterus lumpus). J Vet Diagn Invest 24(3):516–524. https://doi.org/10.1177/1040638712440990 CrossRefPubMed

Freeman MA, Kasper JM, Kristmundsson A (2013) Nucleospora cyclopteri n. sp., an intranuclear microsporidian infecting wild lumpfish, Cyclopterus lumpus L., in Icelandic waters. Parasit Vectors 6:49. https://doi.org/10.1186/1756-3305-6-49 CrossRefPubMedPubMedCentral

Gulla S, Sorum H, Vagnes O, Colquhoun DJ (2015) Phylogenetic analysis and serotyping of Vibrio splendidus-related bacteria isolated from salmon farm cleaner fish. Dis Aquat Organ 117(2):121–131. https://doi.org/10.3354/dao02938 CrossRefPubMed

10.

Marcos-Lopez M, Donald K, Stagg H, McCarthy U (2013) Clinical Vibrio anguillarum infection in lumpsucker Cyclopterus lumpus in Scotland. Vet Rec 173(13):319. https://doi.org/10.1136/vr.101763 CrossRefPubMed

11.

Scholz F, Fringuelli E, Bolton-Warberg M, Marcos-Lopez M, Mitchell S, Prodhol P, Moffet D, Savage P, Murphy O’Sullivan S, O’Connor I, McCarthy E, Rodger HD (2017) First record of Tetramicra brevifilum in lumpfish (Cyclopterus lumpus, L.). J Fish Dis 40(6):757–771. https://doi.org/10.1111/jfd.12554 CrossRefPubMed

12.

Haugland GT, Olsen AB, Rønneseth A, Andersen L (2016) Lumpfish (Cyclopterus lumpus L.) develop amoebic gill disease (AGD) after experimental challenge with Paramoeba perurans and can transfer amoebae to Atlantic salmon (Salmo salar L.). Aquaculture 478:48–55CrossRef

13.

Mullins EJ, Powell M, Speare DJ, Cawthorn R (1994) An intranuclearmicrosporidian in lumpfish Cyclopterus lumpus. Dis Aquat Organ 20:7–13CrossRef

14.

Småge SB, Frisch K, Brevik ØJ, Watanabe K, Nylund A (2016) First isolation, identification and characterisation of Tenacibaculum maritimum in Norway, isolated from diseased farmed sea lice cleaner fish Cyclopterus lumpus L. Aquaculture 464:178–184CrossRef

15.

Steigen A, Nylund A, Karlsbakk E, Akoll P, Fiksdal IU, Nylund S, Odong R, Plarre H, Semyalo R, Skar C, Watanabe K (2013) ‘Cand. Actinochlamydia clariae’ gen. nov., sp. nov., a unique intracellular bacterium causing epitheliocystis in catfish (Clarias gariepinus) in Uganda. PLoS One 8(6):e66840. https://doi.org/10.1371/journal.pone.0066840 CrossRefPubMedPubMedCentral

16.

Lannan CN, Winton JR, Fryer JL (1984) Fish cell lines: establishment and characterization of nine cell lines from salmonids. Vitro 20(9):671–676CrossRef

17.

Devold M, Krossoy B, Aspehaug V, Nylund A (2000) Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis Aquat Organ 40(1):9–18. https://doi.org/10.3354/dao040009 CrossRefPubMed

18.

Økland AL, Nylund A, Overgard AC, Blindheim S, Watanabe K, Grotmol S, Arnesen CE, Plarre H (2014) Genomic characterization and phylogenetic position of two new species in Rhabdoviridae infecting the parasitic copepod, salmon louse (Lepeophtheirus salmonis). PLoS One 9(11):e112517. https://doi.org/10.1371/journal.pone.0112517 CrossRefPubMedPubMedCentral

19.

Nolden T, Pfaff F, Nemitz S, Freuling CM, Höper D, Müller T, Finke S (2016) Reverse genetics in high throughput: rapid generation of complete negative strand RNA virus cDNA clones and recombinant viruses thereof. Sci Rep 6:23887. https://doi.org/10.1038/srep23887 ( http://www.nature.com/articles/srep23887#supplementary-information )CrossRefPubMedPubMedCentral

20.

King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy. Ninth report of the International committee on taxonomy of viruses. Academic Press, US

21.

Atkins JF, Loughran G, Bhatt PR, Firth AE, Baranov PV (2016) Ribosomal frameshifting and transcriptional slippage: from genetic steganography and cryptography to adventitious use. Nucleic Acids Res 44(15):7007–7078. https://doi.org/10.1093/nar/gkw530 PubMedPubMedCentral

22.

Firth AE, Blitvich BJ, Wills NM, Miller CL, Atkins JF (2010) Evidence for ribosomal frameshifting and a novel overlapping gene in the genomes of insect-specific flaviviruses. Virology 399(1):153–166. https://doi.org/10.1016/j.virol.2009.12.033 CrossRefPubMedPubMedCentral

23.

Firth AE, Brierley I (2012) Non-canonical translation in RNA viruses. J Gen Virol 93(Pt 7):1385–1409. https://doi.org/10.1099/vir.0.042499-0 CrossRefPubMedPubMedCentral

24.

Walewski JL, Keller TR, Stump DD, Branch AD (2001) Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame. RNA 7(5):710–721CrossRefPubMedPubMedCentral

25.

Stadler K, Allison SL, Schalich J, Heinz FX (1997) Proteolytic activation of tick-borne encephalitis virus by furin. J Virol 71(11):8475–8481PubMedPubMedCentral

26.

de Lamballerie X, Crochu S, Billoir F, Neyts J, de Micco P, Holmes EC, Gould EA (2002) Genome sequence analysis of Tamana bat virus and its relationship with the genus Flavivirus. J Gen Virol 83(Pt 10):2443–2454. https://doi.org/10.1099/0022-1317-83-10-2443 CrossRefPubMed

27.

Grishin NV (1998) The R3H motif: a domain that binds single-stranded nucleic acids. Trends Biochem Sci 23(9):329–330CrossRefPubMed

28.

Talavera G, Castresana J (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol 56(4):564–577. https://doi.org/10.1080/10635150701472164 CrossRefPubMed

29.

Muller T, Vingron M (2000) Modeling amino acid replacement. J Comput Biol 7(6):761–776. https://doi.org/10.1089/10665270050514918 CrossRefPubMed

30.

Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12(4):357–358PubMed

31.

Leung JY, Pijlman GP, Kondratieva N, Hyde J, Mackenzie JM, Khromykh AA (2008) Role of nonstructural protein NS2A in flavivirus assembly. J Virol 82(10):4731–4741. https://doi.org/10.1128/JVI.00002-08 CrossRefPubMedPubMedCentral

32.

Blitvich BJ, Firth AE (2017) A review of Flaviviruses that have no known arthropod vector. Viruses. https://doi.org/10.3390/v9060154 PubMedPubMedCentral

Title: New virus of the family Flaviviridae detected in lumpfish (Cyclopterus lumpus)
Authors: Renate Hvidsten Skoge
Jarle Brattespe
Arnfinn Lodden Økland
Heidrun Plarre
Are Nylund
Publication date: 01-03-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3643-3

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

New virus of the family Flaviviridae detected in lumpfish (Cyclopterus lumpus)

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2018

Significant differences in the intra-host genetic diversity of Helicoverpa armigera nucleopolyhedrovirus dnapol after serial in vivo passages in the same insect population

Complete genome sequence of Paris mosaic necrosis virus, a distinct member of the genus Potyvirus

Characterization of tomato leaf curl purple vein virus, a new monopartite New World begomovirus infecting tomato in Northeast Brazil

Hepatitis C virus infection among illicit drug users in an archipelago of the Amazon

Enhancement of therapeutic DNA vaccine potency by melatonin through inhibiting VEGF expression and induction of antitumor immunity mediated by CD8+ T cells

Characterization of a novel single-stranded RNA mycovirus related to invertebrate viruses from the plant pathogen Verticillium dahliae

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page