Skip to main content
Top
Published in: Archives of Virology 4/2016

01-04-2016 | Brief Report

Genetic heterogeneity of the VP6 gene and predominance of G6P[5] genotypes of Brazilian porcine rotavirus C field strains

Authors: Flávia Possatti, Elis Lorenzetti, Alice Fernandes Alfieri, Amauri Alcindo Alfieri

Published in: Archives of Virology | Issue 4/2016

Login to get access

Abstract

The aim of this study was to investigate the genetic diversity of the VP6, VP7, and VP4 genes of 15 Brazilian wild-type porcine RVC strains identified in diarrheic fecal samples. The VP6 gene analysis demonstrated heterogeneity between the 15 RVC strains, which clustered in three distinct genotypes (I1, I5, and I6). In the VP7 and VP4 gene analysis, the genotype combination G6P[4] was detected in only one strain (UEL-77), while G6P[5] was the most commonly (n = 14) detected in RVC strains identified in the Brazilian pig herds evaluated, indicating its probable predominance in this country, mainly in 2014.
Literature
1.
go back to reference Estes MK, Kapikian AZ (2007) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology, vol 2, 5th edn. Lippincott Williams and Wilkins, Philadelphia, pp 1917–1974 Estes MK, Kapikian AZ (2007) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology, vol 2, 5th edn. Lippincott Williams and Wilkins, Philadelphia, pp 1917–1974
4.
go back to reference Lorenzetti E, Stipp DT, Possatti F, Campanha JET, Alfieri AF, Alfieri AA (2014) Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections. Braz J Vet Res 34:391–397 Lorenzetti E, Stipp DT, Possatti F, Campanha JET, Alfieri AF, Alfieri AA (2014) Diarrhea outbreaks in suckling piglets due to rotavirus group C single and mixed (rotavirus groups A and B) infections. Braz J Vet Res 34:391–397
5.
go back to reference Kim Y, Chang KO, Straw B, Saif LJ (1999) Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs. J Clin Microbiol 37(5):1484–1488PubMedPubMedCentral Kim Y, Chang KO, Straw B, Saif LJ (1999) Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs. J Clin Microbiol 37(5):1484–1488PubMedPubMedCentral
6.
go back to reference Saif LJ, Bohl EH, Theil KW, Cross RF, House JA (1980) Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol 12(1):105–111PubMedPubMedCentral Saif LJ, Bohl EH, Theil KW, Cross RF, House JA (1980) Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol 12(1):105–111PubMedPubMedCentral
8.
go back to reference Marthaler D, Rossow K, Culhane M, Collins J, Goyal S, Ciarlet M, Matthijnssens J (2013) Identification, phylogenetic analysis and classification of porcine group C rotavirus VP7 sequences from the United States and Canada. Virology 446(1–2):189–198. doi:10.1016/j.virol.2013.08.001 CrossRefPubMed Marthaler D, Rossow K, Culhane M, Collins J, Goyal S, Ciarlet M, Matthijnssens J (2013) Identification, phylogenetic analysis and classification of porcine group C rotavirus VP7 sequences from the United States and Canada. Virology 446(1–2):189–198. doi:10.​1016/​j.​virol.​2013.​08.​001 CrossRefPubMed
9.
go back to reference Martella V, Bányai K, Lorusso E, Bellacicco AL, Decaro N, Camero M, Bozzo G, Moschidou P, Arista S, Pezzotti G, Lavazza A, Buonavoglia C (2007) Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis. Vet Microbiol 123(1–3):26–33. doi:10.1016/j.vetmic.2007.03.003 CrossRefPubMed Martella V, Bányai K, Lorusso E, Bellacicco AL, Decaro N, Camero M, Bozzo G, Moschidou P, Arista S, Pezzotti G, Lavazza A, Buonavoglia C (2007) Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis. Vet Microbiol 123(1–3):26–33. doi:10.​1016/​j.​vetmic.​2007.​03.​003 CrossRefPubMed
10.
go back to reference Médici KC, Barry AF, Alfieri AF, Alfieri AA (2011) Porcine rotavirus groups A, B, and C identified by polymerase chain reaction in fecal sample collection with inconclusive results by polyacrylamide gel electrophoresis. J Swine Health Prod 19(3):146–150 Médici KC, Barry AF, Alfieri AF, Alfieri AA (2011) Porcine rotavirus groups A, B, and C identified by polymerase chain reaction in fecal sample collection with inconclusive results by polyacrylamide gel electrophoresis. J Swine Health Prod 19(3):146–150
12.
go back to reference Chang KO, Nielsen PR, Ward LA, Saif LJ (1999) Dual infection of gnotobiotic calves with bovine strains of group A and porcine-like group C rotaviruses influences pathogenesis of the group C rotavirus. J Virol 73(11):9284–9293PubMedPubMedCentral Chang KO, Nielsen PR, Ward LA, Saif LJ (1999) Dual infection of gnotobiotic calves with bovine strains of group A and porcine-like group C rotaviruses influences pathogenesis of the group C rotavirus. J Virol 73(11):9284–9293PubMedPubMedCentral
13.
go back to reference Jeong YJ, Park SI, Hosmillo M, Shin DJ, Chun YH, Kim HJ, Kwon HJ, Kang SY, Woo SK, Park SJ, Kim GY, Kang MI, Cho KO (2009) Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet Microbiol 138(3–4):217–224. doi:10.1016/j.vetmic.2009.03.024 CrossRefPubMed Jeong YJ, Park SI, Hosmillo M, Shin DJ, Chun YH, Kim HJ, Kwon HJ, Kang SY, Woo SK, Park SJ, Kim GY, Kang MI, Cho KO (2009) Detection and molecular characterization of porcine group C rotaviruses in South Korea. Vet Microbiol 138(3–4):217–224. doi:10.​1016/​j.​vetmic.​2009.​03.​024 CrossRefPubMed
14.
go back to reference Gabbay YB, Borges AA, Oliveira DS, Linhares AC, Mascarenhas JD, Barardi CR, Simoes CM, Wang Y, Glass RI, Jiang B (2008) Evidence for zoonotic transmission of group C rotaviruses among children in Belem, Brazil. J Med Virol 80(9):1666–1674. doi:10.1002/jmv.21250 CrossRefPubMed Gabbay YB, Borges AA, Oliveira DS, Linhares AC, Mascarenhas JD, Barardi CR, Simoes CM, Wang Y, Glass RI, Jiang B (2008) Evidence for zoonotic transmission of group C rotaviruses among children in Belem, Brazil. J Med Virol 80(9):1666–1674. doi:10.​1002/​jmv.​21250 CrossRefPubMed
15.
go back to reference Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gomara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82(7):3204–3219. doi:10.1128/jvi.02257-07 CrossRefPubMedPubMedCentral Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gomara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82(7):3204–3219. doi:10.​1128/​jvi.​02257-07 CrossRefPubMedPubMedCentral
17.
go back to reference Moutelíková R, Prodelalová J, Dufková L (2015) Diversity of VP7, VP4, VP6, NSP2, NSP4, and NSP5 genes of porcine rotavirus C: phylogenetic analysis and description of potential new VP7, VP4, VP6, and NSP4 genotypes. Arch Virol 160(7):1715–1727. doi:10.1007/s00705-015-2438-7 CrossRefPubMed Moutelíková R, Prodelalová J, Dufková L (2015) Diversity of VP7, VP4, VP6, NSP2, NSP4, and NSP5 genes of porcine rotavirus C: phylogenetic analysis and description of potential new VP7, VP4, VP6, and NSP4 genotypes. Arch Virol 160(7):1715–1727. doi:10.​1007/​s00705-015-2438-7 CrossRefPubMed
18.
go back to reference Jeong YJ, Matthijnssens J, Kim DS, Kim JY, Alfajaro MM, Park JG, Hosmillo M, Son KY, Soliman M, Baek YB, Kwon J, Choi JS, Kang MI, Cho KO (2015) Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses. Vet Microbiol 176(1–2):61–69. doi:10.1016/j.vetmic.2014.12.024 CrossRefPubMed Jeong YJ, Matthijnssens J, Kim DS, Kim JY, Alfajaro MM, Park JG, Hosmillo M, Son KY, Soliman M, Baek YB, Kwon J, Choi JS, Kang MI, Cho KO (2015) Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses. Vet Microbiol 176(1–2):61–69. doi:10.​1016/​j.​vetmic.​2014.​12.​024 CrossRefPubMed
21.
go back to reference Moutelíková R, Prodělalová J, Dufková L (2013) Prevalence study and phylogenetic analysis of group C porcine rotavirus in the Czech Republic revealed a high level of VP6 gene heterogeneity within porcine cluster I1. Arch Virol 159:1163–1167. doi:10.1007/s00705-013-1903-4 CrossRefPubMed Moutelíková R, Prodělalová J, Dufková L (2013) Prevalence study and phylogenetic analysis of group C porcine rotavirus in the Czech Republic revealed a high level of VP6 gene heterogeneity within porcine cluster I1. Arch Virol 159:1163–1167. doi:10.​1007/​s00705-013-1903-4 CrossRefPubMed
25.
go back to reference Grice AS, Lambden PR, Caul EO, Clarke IN (1994) Sequence conservation of the major outer capsid glycoprotein of human group C rotaviruses. J Med Virol 44(2):166–171CrossRefPubMed Grice AS, Lambden PR, Caul EO, Clarke IN (1994) Sequence conservation of the major outer capsid glycoprotein of human group C rotaviruses. J Med Virol 44(2):166–171CrossRefPubMed
26.
go back to reference Jiang B, Tsunemitsu H, Dennehy PH, Oishi I, Brown D, Schnagl RD, Oseto M, Fang ZY, Avendano LF, Saif LJ, Glass RI (1996) Sequence conservation and expression of the gene encoding the outer capsid glycoprotein among human group C rotaviruses of global distribution. Arch Virol 141(2):381–390CrossRefPubMed Jiang B, Tsunemitsu H, Dennehy PH, Oishi I, Brown D, Schnagl RD, Oseto M, Fang ZY, Avendano LF, Saif LJ, Glass RI (1996) Sequence conservation and expression of the gene encoding the outer capsid glycoprotein among human group C rotaviruses of global distribution. Arch Virol 141(2):381–390CrossRefPubMed
27.
go back to reference Marton S, Mihalov-Kovacs E, Doro R, Csata T, Feher E, Oldal M, Jakab F, Matthijnssens J, Martella V, Banyai K (2015) Canine rotavirus C strain detected in Hungary shows marked genotype diversity. J Gen Virol 96(10):3059–3071. doi:10.1099/jgv.0.000237 CrossRefPubMed Marton S, Mihalov-Kovacs E, Doro R, Csata T, Feher E, Oldal M, Jakab F, Matthijnssens J, Martella V, Banyai K (2015) Canine rotavirus C strain detected in Hungary shows marked genotype diversity. J Gen Virol 96(10):3059–3071. doi:10.​1099/​jgv.​0.​000237 CrossRefPubMed
28.
go back to reference Alfieri AA, Leite JP, Alfieri AF, Jiang B, Glass RI, Gentsch JR (1999) Detection of field isolates of human and animal group C rotavirus by reverse transcription-polymerase chain reaction and digoxigenin-labeled oligonucleotide probes. J Virol Methods 83(1–2):35–43CrossRefPubMed Alfieri AA, Leite JP, Alfieri AF, Jiang B, Glass RI, Gentsch JR (1999) Detection of field isolates of human and animal group C rotavirus by reverse transcription-polymerase chain reaction and digoxigenin-labeled oligonucleotide probes. J Virol Methods 83(1–2):35–43CrossRefPubMed
29.
go back to reference Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16(3):473–477PubMedPubMedCentral Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16(3):473–477PubMedPubMedCentral
30.
go back to reference Pereira HG, Azeredo RS, Leite JP, Candeias JA, Racz ML, Linhares AC, Gabbay YB, Trabulsi JR (1983) Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, Sao Paulo and Para, Brazil. J Hyg 90(1):117–125CrossRefPubMedPubMedCentral Pereira HG, Azeredo RS, Leite JP, Candeias JA, Racz ML, Linhares AC, Gabbay YB, Trabulsi JR (1983) Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, Sao Paulo and Para, Brazil. J Hyg 90(1):117–125CrossRefPubMedPubMedCentral
31.
go back to reference Alfieri AA, Parazzi ME, Takiuchi E, Medici KC, Alfieri AF (2006) Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002. Trop Anim Health Prod 38(7–8):521–526CrossRefPubMed Alfieri AA, Parazzi ME, Takiuchi E, Medici KC, Alfieri AF (2006) Frequency of group A rotavirus in diarrhoeic calves in Brazilian cattle herds, 1998–2002. Trop Anim Health Prod 38(7–8):521–526CrossRefPubMed
32.
go back to reference Martella V, Banyai K, Lorusso E, Decaro N, Bellacicco A, Desario C, Corrente M, Greco G, Moschidou P, Tempesta M, Arista S, Ciarlet M, Lavazza A, Buonavoglia C (2007) Genetic heterogeneity in the VP7 of group C rotaviruses. Virology 367(2):358–366. doi:10.1016/j.virol.2007.05.039 CrossRefPubMed Martella V, Banyai K, Lorusso E, Decaro N, Bellacicco A, Desario C, Corrente M, Greco G, Moschidou P, Tempesta M, Arista S, Ciarlet M, Lavazza A, Buonavoglia C (2007) Genetic heterogeneity in the VP7 of group C rotaviruses. Virology 367(2):358–366. doi:10.​1016/​j.​virol.​2007.​05.​039 CrossRefPubMed
Metadata
Title
Genetic heterogeneity of the VP6 gene and predominance of G6P[5] genotypes of Brazilian porcine rotavirus C field strains
Authors
Flávia Possatti
Elis Lorenzetti
Alice Fernandes Alfieri
Amauri Alcindo Alfieri
Publication date
01-04-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 4/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2750-x

Other articles of this Issue 4/2016

Archives of Virology 4/2016 Go to the issue