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Archives of Virology
Published in: Archives of Virology 2/2013

01-02-2013 | Brief Report

Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)

Authors: N. Bejerman, F. Giolitti, S. de Breuil, S. Lenardon

Published in: Archives of Virology | Issue 2/2013

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Abstract

A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
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Literature
1.
Adams MJ (2012) Carstens EB (2012) Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses. Arch Virol 157:1411–1422PubMedCrossRef
2.
Ahlquist P, French R, Janda M, Loesch-Fries L (1984) Multi component RNA plant virus infection derived from cloned viral cDNA. Proc Natl Acad Sci USA 81:7066–7070PubMedCrossRef
3.
Bedoya L, Daros JA (2010) Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Res 149:234–240PubMedCrossRef
4.
Bejerman N, Giolitti F, de Breuil S, Lenardon S (2010) Molecular characterization of Sunflower chlorotic mottle virus: a member of a distinct species in the genus Potyvirus. Arch Virol 155:1331–1335PubMedCrossRef
5.
Boyer JC, Haenni AL (1994) Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415–426PubMedCrossRef
6.
Chiang CH, Yeh SD (1997) Infectivity assays of in vitro and in vivo transcripts of Papaya ringspot virus. Bot Bull Acad Sinica. 38:153–163
7.
Chung B, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902PubMedCrossRef
8.
Del Sal G, Manfioletti G, Schneider C (1989) The CTAB-DNA Precipitation Method: A common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. BioTechniques 7:514–519PubMed
9.
Dujovny G, Usugi T, Shohara K, Lenardon SL (1998) Characterization of a new Potyvirus infecting sunflower in Argentina. Plant Dis 82:470–474CrossRef
10.
Dujovny G, Sasaya T, Koganezawa H, Usugi T, Shohara K, Lenardon SL (2000) Molecular characterization of a new Potyvirus infecting sunflower. Arch Virol 145:2249–2258PubMedCrossRef
11.
Gal-On A, Meiri E, Huet H, Hau JW, Raccah B, Gaba V (1995) Particle bombardment drastically increases the infectivity of cloned DNA of Zucchini yellow mosaic potyvirus. J Gen Virol 76:3223–3227PubMedCrossRef
12.
Giolitti F, Bejerman N, de Breuil S, Lenardon S (2010) Identification and characterization of a new strain of Sunflower chlorotic mottle virus, a Potyvirus infecting Asteraceae in Argentina. J Phytopathol 158:536–541CrossRef
13.
Jakab G, Droz E, Brigneti G, Baulcombe D, Malnoe P (1997) Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of Potato virus Y. J Gen Virol 78:3141–3145PubMed
14.
Johansen IE (1996) Intron insertion facilitates amplification of cloned virus cDNA in Escherichia coli while biological activity is re-established after transcription in vivo. Proc Natl Acad Sci USA 93:12400–12405PubMedCrossRef
15.
Lee MY, Song YS, Ryu KH (2011) Development of infectious transcripts from full-length and GFP-tagged cDNA clones of Pepper mottle virus and stable systemic expression of GFP in tobacco and pepper. Virus Res 155:487–494PubMedCrossRef
16.
Lenardon SL, Bazzalo ME, Abratti G, Cimino C, Galella MT, Grondona M, Giolitti F, León AJ (2005) Screening sunflower for resistance to Sunflower chlorotic mottle virus and mapping the Rcmo-1 resistance gene. Crop Sci 45:735–739CrossRef
17.
Lin SS, Roger FH, Yeh SD (2002) Construction of in vivo and in vitro infectious transcripts of a Taiwan strain of Zucchini yellow mosaic virus. Bot Bull Acad Sinica 43:261–269
18.
Lopez-Moya JJ, García JA (2000) Construction of a stable and highly infectious intron-containing cDNA clone of Plum pox potyvirus and its use to infect plants by particle bombardment. Virus Res 68:99–107PubMedCrossRef
19.
Maiss E, Timpe U, Brisske-Rode A, Lesemann DE, Casper R (1992) Infectious in vivo transcripts of a Plum pox potyvirus full-length cDNA clone containing the Cauliflower mosaic virus 35S RNA promoter. J Gen Virol 73:709–713PubMedCrossRef
20.
Masuta C, Yamana T, Tacahashi Y, Uyeda I, Sato M, Uyeda S, Matsumara T (2000) Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species. Plant J 23:539–546PubMedCrossRef
21.
Nagyová A, Subr Z (2007) Infectious full-length clones of plant viruses and their use for construction of viral vector. Acta Virol 51:223–237PubMed
22.
Nicolas O, Pirone TP, Hellmann GM (1996) Construction and analysis of infectious transcripts from a resistance-breaking strain of Tobacco vein mottling potyvirus. Arch Virol 141:1535–1552PubMedCrossRef
23.
Puurand Ü, Valkonen JPT, Makinen K, Rabenstein F, Saarma M (1996) Infectious in vitro transcripts from cloned cDNA of the Potato A potyvirus. Virus Res 40:135–140PubMedCrossRef
24.
Sanchez F, Martinez-Herrera D, Aguilar I, Ponz F (1998) Infectivity of Turnip mosaic potyvirus cDNA clones and transcripts on the systemic host Arabidopsis thaliana and local lesion hosts. Virus Res 55:207–219PubMedCrossRef
25.
Seo J-K, Lee H-G, Choi H-S, Lee S-H, Kim K-H (2009) Infectious in vivo transcripts from a full-length clone of Soybean mosaic virus strain G5H. Plant Pathol. J. 25:54–61CrossRef
26.
Shukla DD, Ward CW, Brunt AA (1994) The Potyviridae. CAB International, Wallingford, U.K.
27.
Skuzeski JM, Nichols LM, Gesteland RF (1990) Analysis of leaky viral translation termination codons in vivo by transient expression of improved β-glucuronidase vectors. Plant Mol Biol 15:65–79PubMedCrossRef
28.
Stewart LR, Bouchard R, Redinbaugh MG, Meulia T (2012) Complete sequence and development of a full-length infectious clone of an Ohio isolate of Maize dwarf mosaic virus (MDMV). Virus Res 165:219–224PubMedCrossRef
29.
Tsuneyoshi T, Matsumi T, Deng TC, Sako I, Sumi S (1998) Differentiation of Allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence. Arch Virol 143:1093–1107PubMedCrossRef
30.
Yang SG, Revers F, Souche S, Lot H, Le Gall OL, Candresse T, Dunez J (1998) Construction of full-length cDNA clones of Lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants. Arch. Virol 143:2443–2451PubMedCrossRef
31.
Milne RG, Luisoni E (1977) Rapid immune electron microscopy of virus preparations. Methods Virol 6:265–281
Metadata
Title
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
Authors
N. Bejerman
F. Giolitti
S. de Breuil
S. Lenardon
Publication date
01-02-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1497-2

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