Skip to main content
Top
Published in: Comparative Clinical Pathology 5/2018

01-09-2018 | Original Article

Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice

Authors: Mohammad Mahdi Zangeneh, Akram Zangeneh, Hossein Amiri, Nassim Amiri, Reza Tahvilian, Rohallah Moradi, Hossein Zhaleh, Payam Razeghi Tehrani

Published in: Comparative Clinical Pathology | Issue 5/2018

Login to get access

Abstract

Using ethno-medicinal plants is the oldest way of mankind to treat the several diseases. Due to the rapid growth of global interest in use of medicinal plants, their effects and safety evaluation have become substantial. In this study, Alyssum meniocoides (AM) Boiss aqueous extract was extracted to investigate its nephroprotective activity on renal structural and biochemical changes in streptozotocin-induced diabetic nephrotoxicity in male mice. In this study, 80 mice were used. Diabetes was experimentally induced by intraperitoneal injection of streptozotocin (STZ) in 70 mice. Fasting blood glucose (FBG) levels were assessed everyday by glucometer strips. Mice with plasma glucose level > 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into 8 groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 40 mg/kg glibenclamide orally. Groups 4, 5, 6, 7, and 8 were given 10, 20, 40, 80, and 160 mg/kg, respectively of AM for 20 days orally. At the 20th day, the mice were dissected, blood and kidney samples collected for biochemical and histological analysis. Histologically, several doses of AM could significantly (p ≤ 0.05) decrease the volume and length of the renal structures as compared to the untreated group. Biochemically, AM at all doses could significantly (p ≤ 0.05) reduce the raised levels of urea and creatinine and increased SOD and catalase (CAT) levels as compared to the untreated group. In conclusion, AM has nephroprotective property, thereby reducing the causation of diabetes in experimental mice.
Literature
go back to reference Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684CrossRef Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684CrossRef
go back to reference Braendgaard H, Gundersen HJ (1986) The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods 18(1–2):39–78CrossRefPubMed Braendgaard H, Gundersen HJ (1986) The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods 18(1–2):39–78CrossRefPubMed
go back to reference Breyer MD, Bottinger E, Brosius FC, Coffman TM, Harris RC, Heilig CW, Sharma K, AMDCC (2005) Mouse models of diabetic nephropathy. J Am Soc Nephrol 16(1):27–45CrossRefPubMed Breyer MD, Bottinger E, Brosius FC, Coffman TM, Harris RC, Heilig CW, Sharma K, AMDCC (2005) Mouse models of diabetic nephropathy. J Am Soc Nephrol 16(1):27–45CrossRefPubMed
go back to reference Brosius FC, Alpers CE, Bottinger EP, Breyer MD, Coffman TM, Gurley SB, Harris RC, Kakoki M, Kretzler M, Leiter EH, Levi M, McIndoe RA, Sharma K, Smithies O, Susztak K, Takahashi N, Takahashi T (2009) Mouse models of diabetic nephropathy. J Am Soc Nephrol 20(12):2503–2512CrossRefPubMedPubMedCentral Brosius FC, Alpers CE, Bottinger EP, Breyer MD, Coffman TM, Gurley SB, Harris RC, Kakoki M, Kretzler M, Leiter EH, Levi M, McIndoe RA, Sharma K, Smithies O, Susztak K, Takahashi N, Takahashi T (2009) Mouse models of diabetic nephropathy. J Am Soc Nephrol 20(12):2503–2512CrossRefPubMedPubMedCentral
go back to reference Dhodi DK, Bhagat SB, Pathak D, Patel SB (2014) Drug-induced nephrotoxicity. Int J Basic Clin Pharmacol 3(4):591–597CrossRef Dhodi DK, Bhagat SB, Pathak D, Patel SB (2014) Drug-induced nephrotoxicity. Int J Basic Clin Pharmacol 3(4):591–597CrossRef
go back to reference Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2016) Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Current Med Pharm Res 7(6):367–371 Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2016) Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Current Med Pharm Res 7(6):367–371
go back to reference Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2017) Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rew Res 8(1):13–17 Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2017) Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rew Res 8(1):13–17
go back to reference Ghaderiana SM, Mohtadia A, Rahiminejada MR, Baker AJM (2007) Nickel and other metal uptake and accumulation by species of Alyssum (Brassicaceae) from the ultramafics of Iran. Environ Pollut 145(1):293–298CrossRef Ghaderiana SM, Mohtadia A, Rahiminejada MR, Baker AJM (2007) Nickel and other metal uptake and accumulation by species of Alyssum (Brassicaceae) from the ultramafics of Iran. Environ Pollut 145(1):293–298CrossRef
go back to reference Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRef Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRef
go back to reference Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium Saralicum R.M. Fritsch on CCl4-induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3):227–238 Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium Saralicum R.M. Fritsch on CCl4-induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3):227–238
go back to reference Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium Saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Univ 25(132):21–30 Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium Saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Univ 25(132):21–30
go back to reference Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A (1992) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Acta pathologica microbiologica immunologica. Scandinavica 96(5):379–394 Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A (1992) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Acta pathologica microbiologica immunologica. Scandinavica 96(5):379–394
go back to reference Hachi M, Ouafae B, Hachi T, Mohamed EB, Imane B, Atmane R, Zidane L (2016) Contribution to the ethnobotanical study of antidiabetic medicinal plants of the Central Middle Atlas region (Morocco). Lazaroa 37:135–144CrossRef Hachi M, Ouafae B, Hachi T, Mohamed EB, Imane B, Atmane R, Zidane L (2016) Contribution to the ethnobotanical study of antidiabetic medicinal plants of the Central Middle Atlas region (Morocco). Lazaroa 37:135–144CrossRef
go back to reference Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRef Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRef
go back to reference Kraynak AR, Storer RD, Jensen RD, Kloss MW, Soper KA, Clair JH, DeLuca JG, Nichols WW, Eydelloth RS (1995) Extent and persistence of streptozotocin-induced DNAdamage and cell proliferation in rat kidney as determined by in vivo alkaline elution and BrdUrd labeling assays. Toxicol Appl Pharmacol 135(2):279–286CrossRefPubMed Kraynak AR, Storer RD, Jensen RD, Kloss MW, Soper KA, Clair JH, DeLuca JG, Nichols WW, Eydelloth RS (1995) Extent and persistence of streptozotocin-induced DNAdamage and cell proliferation in rat kidney as determined by in vivo alkaline elution and BrdUrd labeling assays. Toxicol Appl Pharmacol 135(2):279–286CrossRefPubMed
go back to reference Le-Devehat C, Khodabandehlou T, Vimeux M (2001) Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc 25(2):43–48PubMed Le-Devehat C, Khodabandehlou T, Vimeux M (2001) Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc 25(2):43–48PubMed
go back to reference Lenzen S (2008) The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 51(2):216–226CrossRefPubMed Lenzen S (2008) The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 51(2):216–226CrossRefPubMed
go back to reference Mandarim-de-Lacerda CA (2003) Stereological tools in biomedical research. An Acad Bras Cienc 75(4):469–486CrossRefPubMed Mandarim-de-Lacerda CA (2003) Stereological tools in biomedical research. An Acad Bras Cienc 75(4):469–486CrossRefPubMed
go back to reference Martin JP Jr, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336CrossRefPubMed Martin JP Jr, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336CrossRefPubMed
go back to reference Mishra S, Ranjan-Pani S, Sahoo S (2014) Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha. Pharm Res 6(3):210–217 Mishra S, Ranjan-Pani S, Sahoo S (2014) Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha. Pharm Res 6(3):210–217
go back to reference Mohana-Lakshmi S, Usha-Kiran-Reddy T, Sandhya-Rani KS (2012) A review on medicinal plants for nephroprotective activity. Asian J Pharm Clin Res 5(4):8–14 Mohana-Lakshmi S, Usha-Kiran-Reddy T, Sandhya-Rani KS (2012) A review on medicinal plants for nephroprotective activity. Asian J Pharm Clin Res 5(4):8–14
go back to reference Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A (2017) Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res 3(2):1360–1362 Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A (2017) Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res 3(2):1360–1362
go back to reference Najafi F, Tahvilian R, Zangeneh MM, Zangeneh A, Moradi R (2016) Screening of essential oil of Allium sativum for antibacterial effects against Bacillus subtilis. Int J Rec Sci Res 7(11):14172–14176 Najafi F, Tahvilian R, Zangeneh MM, Zangeneh A, Moradi R (2016) Screening of essential oil of Allium sativum for antibacterial effects against Bacillus subtilis. Int J Rec Sci Res 7(11):14172–14176
go back to reference Najafi F, Goodarzi N, Zangeneh MM, Zangeneh A, Hagh-Nazari L (2017) Antidiabtic and hepatoprotective effects of bitter fraction of Stevia rebaudiana alcoholic extract on streptozotocin-induced diabetic male mice. J Rafsanjan Univ Med Sci 16(6):493–504 Najafi F, Goodarzi N, Zangeneh MM, Zangeneh A, Hagh-Nazari L (2017) Antidiabtic and hepatoprotective effects of bitter fraction of Stevia rebaudiana alcoholic extract on streptozotocin-induced diabetic male mice. J Rafsanjan Univ Med Sci 16(6):493–504
go back to reference Palm F, Ortster H, Hansell P, Liss P, Carlsson PO (2004) Differentiating between effects of streptozotocin per se and subsequent hyperglycemia on renal function and metabolism in the streptozotocin diabetic rat model. Diabetes Metab Res Rev 20(6):452–459CrossRefPubMed Palm F, Ortster H, Hansell P, Liss P, Carlsson PO (2004) Differentiating between effects of streptozotocin per se and subsequent hyperglycemia on renal function and metabolism in the streptozotocin diabetic rat model. Diabetes Metab Res Rev 20(6):452–459CrossRefPubMed
go back to reference Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A (2017) Cucurbita moschata: a plant with antibacterial properties. Int J Current Med Pharm Res 3(2):1356–1359 Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A (2017) Cucurbita moschata: a plant with antibacterial properties. Int J Current Med Pharm Res 3(2):1356–1359
go back to reference Pooyanmehr M, Zangeneh MM, Zangeneh A, Almasi M (2017) Effect of Verbascum thapsus aqueous extract on Escherichia coli O157:H7. Online J Vet Res 21(9):580–583 Pooyanmehr M, Zangeneh MM, Zangeneh A, Almasi M (2017) Effect of Verbascum thapsus aqueous extract on Escherichia coli O157:H7. Online J Vet Res 21(9):580–583
go back to reference Rerup CC (1970) Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev 22(4):485–518PubMed Rerup CC (1970) Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev 22(4):485–518PubMed
go back to reference Schetz M, Dasta J, Goldstein S, Golper T (2005) Drug-induced acute kidney injury. Curr Opin Crit Care 11(6):555–565CrossRefPubMed Schetz M, Dasta J, Goldstein S, Golper T (2005) Drug-induced acute kidney injury. Curr Opin Crit Care 11(6):555–565CrossRefPubMed
go back to reference Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRef Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRef
go back to reference Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M (2017) Chemical composition and screening of antibacterial activity of essential oil of Pistacia khinjuk against two selected pathogenic bacteria. Ann Trop Med Public Health 10(5):1159–1164CrossRef Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M (2017) Chemical composition and screening of antibacterial activity of essential oil of Pistacia khinjuk against two selected pathogenic bacteria. Ann Trop Med Public Health 10(5):1159–1164CrossRef
go back to reference Tay YC, Wang Y, Kairaitis L, Rangan GK, Zhang C, Harris DCH (2005) Can murine diabetic nephropathy be separated from superimposed acute renal failure? Kidney Int 68(1):391–398CrossRefPubMed Tay YC, Wang Y, Kairaitis L, Rangan GK, Zhang C, Harris DCH (2005) Can murine diabetic nephropathy be separated from superimposed acute renal failure? Kidney Int 68(1):391–398CrossRefPubMed
go back to reference Tesch GH, Allen TJ (2007) Rodent models of streptozotocin induced diabetic nephropathy. Nephrology (Carlton) 12(3):261–266CrossRef Tesch GH, Allen TJ (2007) Rodent models of streptozotocin induced diabetic nephropathy. Nephrology (Carlton) 12(3):261–266CrossRef
go back to reference Tiong HY, Huang P, Xiong S, Li Y, Vathsala A, Zink D (2014) Drug-induced nephrotoxicity: clinical impact and preclinical in vitro models. Mol Pharm 11(7):1933–1948CrossRefPubMed Tiong HY, Huang P, Xiong S, Li Y, Vathsala A, Zink D (2014) Drug-induced nephrotoxicity: clinical impact and preclinical in vitro models. Mol Pharm 11(7):1933–1948CrossRefPubMed
go back to reference Weiss RB (1982) Streptozocin: a review of its pharmacology, efficacy, and toxicity. Cancer Treat Rep 66(3):427–438PubMed Weiss RB (1982) Streptozocin: a review of its pharmacology, efficacy, and toxicity. Cancer Treat Rep 66(3):427–438PubMed
go back to reference Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R (2017) Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem 6(1):251–259 Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R (2017) Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem 6(1):251–259
Metadata
Title
Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice
Authors
Mohammad Mahdi Zangeneh
Akram Zangeneh
Hossein Amiri
Nassim Amiri
Reza Tahvilian
Rohallah Moradi
Hossein Zhaleh
Payam Razeghi Tehrani
Publication date
01-09-2018
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 5/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-018-2712-7

Other articles of this Issue 5/2018

Comparative Clinical Pathology 5/2018 Go to the issue