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01-09-2009 | Original Article

T regulatory cell function in idiopathic minimal lesion nephrotic syndrome

Authors: Carlos Araya, Leila Diaz, Clive Wasserfall, Mark Atkinson, Wei Mu, Richard Johnson, Eduardo Garin

Published in: Pediatric Nephrology | Issue 9/2009

Abstract

The purpose of this study was to test the hypothesis that, in idiopathic minimal lesion nephrotic syndrome (IMLNS), the T regulatory (T reg) cell suppressor mechanism is deficient, thereby enhancing cytokine release by T effector cells. Twenty-one patients with IMLNS, eight healthy controls and two patients with nephrotic syndrome and membranoproliferative glomerulonephritis were studied. The percentage of T reg cells was similar in the healthy controls and in patients with IMLNS in relapse or in remission. Thymidine incorporation in autologous T effector cells, as well as expression of the regulatory cytokine interleukin (IL)-10, was significantly reduced in patients in relapse when compared with patients in remission and healthy subjects. IL-2 expression was also reduced in patients in relapse but did not achieve statistical significance. In a different set of experiments, T cells, from subjects with IMLNS in remission, when stimulated with antiCD3-antiCD28 antibodies, secreted increased levels of cytokines. No such increase in cytokines was observed when cells from healthy controls were stimulated with same mitogen. The impaired T reg cell function observed in these patients may have pathogenic and therapeutic implications, because it could explain the persistence of the proposed pathogenic cytokines observed in the patients with IMLNS.

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Title: T regulatory cell function in idiopathic minimal lesion nephrotic syndrome
Authors: Carlos Araya
Leila Diaz
Clive Wasserfall
Mark Atkinson
Wei Mu
Richard Johnson
Eduardo Garin
Publication date: 01-09-2009
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Nephrology / Issue 9/2009
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-009-1214-x

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T regulatory cell function in idiopathic minimal lesion nephrotic syndrome

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