Abstract
This study was designed to investigate T-lymphocyte subsets interleukin-2 receptor (IL-2R) expression and IL-2 production in minimal change nephrotic syndrome (MCNS). Peripheral blood T-lymphocytes and IL-2R expression were analysed using fluorescein isothiocyanatelabelled CD3, CD4, CD8 and CD25 monoclonal antibodies with flow cytometry. IL-2 production was determined by enzyme immunoassay. Ten children with MCNS in relapse and in remission were evaluated. Thirteen healthy children served as controls. The patients in relapse demonstrated a moderate decrease in the total absolute lymphocyte counts and CD8(+) T-lymphocytes compared with controls (P<0.05) and had a greatly increased IL-2R expression in frashly isolated, unstimulated peripheral lymphocytes compared with patients in remission and controls. While this was not statistically significant, IL-2R expression on cultured lymphocytes stimulated with phytohaemagglutinin was significantly elevated in relapse compared with those in remission and controls (P<0.05). IL-2 production did not correlate well with IL-2R expression and there was no significant difference between the groups. Our results suggest that T-cell subset changes and high IL-2R expression on peripheral lymphocytes may indicate the presence of stimulated T-cell populations in MCNS which could contribute to the immunopathogenesis.
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References
Shalhoub RJ (1974) Pathogenesis of lipoid nephrosis. Lancet II: 557–560
Schnaper HW (1989) The immune system in minimal change nephrotic syndrome. Pediatr Nephrol 3:101–110
Bakker WW, Luijk WJ van (1989) Do circulating factors play a role in the pathogenesis of minimal change nephrotic syndrome. Pediatr Nephrol 3:341–349
Böyum A (1968) Separation of leucocytes from blood and bone marrow with special reference to factors which influence and modify sedimentation properties of hematopoietic cells. Scand J Clin Lab Invest 21 [Suppl 97]:1–11
Ridgway D, Borzy MS (1989) Elevated production of interleukin-2 by lymphocytes from children with acute leukemia. J Pediatr 114:384–391
Cagnoli L, Tabacchi P, Pasquali S, Cenci M, Sandelli M, Zucchelli P (1982) T cell subset alterations in idiopathic glomerulonephritis. Clin Exp Immunol 50:70–76
Herrod HG, Stapleton FB, Trouy RL, Roy S (1983) Evaluation of T lymphocyte subpopulations in children with nephrotic syndrome. Clin Exp Immunol 52:581–585
Yokoyama H, Kida H, Tani Y, Abe T, Tomosugi N, Koshino Y, Hattori N (1985) Immunodynamics of minimal change nephrotic syndrome in adult T and B lymphocyte subsets and serum immunoglobulin levels. Clin Exp Immunol 61:601–607
Yamada H, Dohi K, Morita H, Hirayama T, Takai M, Fujh Y, Ishikawa H, Iwaki K (1987), Two color immunofluorescence and flow cytometry analysis lymphocytes in minimal change nephrotic syndrome. Jpn J Viceral Organs 29:675–686
Fiser RT, Arnold WC, Charlton RK, Steele RW, Childress SH, Shirkey B (1991) T lymphocyte subsets in nephrotic syndrome. Kidney Int 40:913–916
Oppenheim JJ, Ruscethi FW, Faltynek C (1991) Cytokines. In: Stites DP, Terr AI (eds) Basic and clinical immunology. Lange, Connecticut, pp 78–100
Luger TA, Smolen JJ, Chused TM, Steinberg AD, Oppenheim JJ (1982) Human lymphocytes with either OKT4+ and OKT8+ phenotype produced IL-2 in culture. J Clin Invest 70:470–473
Meuer SC, Hussey RE, Penta AC, Fitzgerald KA, Stadler BM, Scholossman SF, Reinherz EL (1982) Evidence for stimulus restricted IL-2 production by OKT4+ and OKT8+ T lymphocytes. J Immunol 129:1076–1079
Kaye WA, Adri MNS, Soeldner JS, Rabinowe SL, Kaldany A, Kahn CR, Bistriam B, Srikante S, Ganda OP, Eisenbarth GS (1986) Acquired defect in interleukin 2 production in patients with type I diabetes mellitus. N Engl J Med 315:920–924
Wakasugi H, Bertoglio J, Turtz T, Frandelizi D (1985) IL-2 receptor induction on human T lymphocytes. Role for IL-2 and monocytes. J Immunol 135:321–327
Hatakeyama M, Minamoto S, Uchiyama T, Hardy RR, Yamada G, Taniguchi T (1985) Reconstitution of functional receptor for human interleukin-2 mouse cells. Nature 318:467–470
Lai KN, Lai FM, Chui SH, Chan YM, Tsao GSW, Leung KN, Lam CWK (1987) Studies of lymphocyte subpopulations and immunoglobulin production in IgA nephropathy. Clin Nephrol 28: 281–287
Lai KN, Leung JCK, Lai FM (1991) Soluble interieukin-2 receptor release, interleukin-2 production and interleukin-2 receptor expression in activated T-lymphocytes in vitro. Pathology 22: 224–228
Lai KN, Leung JCK, Lai FM (1988) In vivo study of expression in T-lymphocytes from patients with IgA nephropathy. Clin Nephrol 30:330–334
Lai KN, Leung JCK, Lai FM, Tam JS (1989) T-lymphocyte activation in IgA nephropathy: serum-soluble interleukin-2 receptor level, interleukin-2 production, and interleukin-2 receptor expression by cultured lymphocytes. J Clin Immunol 9:485–492
Dohi K, Morita H, Ogawa S, Hirayama T, Yamada H, Ishikawa H (1991) Production of interleukin-2 (IL-2) and responsiveness to IL-2 of peripheral blood lymphocytes in minimal change nephrotic syndrome. Jpn J Med 30:396–401
Hinoshita I, Noma T, Tomura S, Shiigai T, Yata J (1990) Decreased production and responsiveness of interleukin-2 in lymphocytes of patients with nephrotic sydrome. Nephron 54:122–126
Suranyi MG, Guasch A, Hall BM, Myers BD (1993) Elevated levels of tumor necrosis factor-α in the nephrotic syndrome in humans. Am J Kidney Dis 21:251–259
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Topaloĝlu, R., Saatçi, Ü., Arikan, M. et al. T-cell subsets, interleukin-2 receptor expression and production of interleukin-2 in minimal change nephrotic syndrome. Pediatr Nephrol 8, 649–652 (1994). https://doi.org/10.1007/BF00869075
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DOI: https://doi.org/10.1007/BF00869075