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European Journal of Nutrition

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01-03-2020 | Alkaloids | Original Contribution

Identification of potential human urinary biomarkers for tomato juice intake by mass spectrometry-based metabolomics

Authors: Yannick Hövelmann, Annika Jagels, Robin Schmid, Florian Hübner, Hans-Ulrich Humpf

Published in: European Journal of Nutrition | Issue 2/2020

Abstract

Purpose

Dietary biomarkers allow the accurate and objective determination of the dietary intake of humans and can thus be valuable for investigating the relation between consumption of foods and biochemical as well as physiological responses. The objective of this study was the identification of potential urinary biomarkers for consumption of tomato juice.

Methods

In the course of a dietary intervention study, the human urine metabolome of a study cohort was compared between a tomato-free diet and after intake of tomato juice by application of an LC-HRMS-based metabolomics approach. The data acquisition was achieved using an orbitrap mass spectrometer, followed by multistage data processing and univariate as well as multivariate statistical analysis to identify discriminating features.

Results

Statistical analysis revealed several unique features detectable after tomato juice intake. The most discriminating markers were putatively identified as hydroxylated and sulfonated metabolites of esculeogenin B, aglycone of the steroidal glycoalkaloid esculeoside B recently found in tomato juice. Furthermore, the β-carboline alkaloids tangutorid E and F and glucuronidated derivatives thereof were identified in urine.

Conclusions

Steroidal glycoalkaloids in tomato juice are cleaved after ingestion, and hydroxylated and sulfonated metabolites of their aglycones might serve as urinary biomarkers for tomato juice intake. Similarly, β-carboline alkaloids and glucuronidated derivatives were identified as potential urinary biomarkers. Both the aglycones of the steroidal alkaloids and the β-carboline alkaloids might exhibit biological activities worth investigating.

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Title: Identification of potential human urinary biomarkers for tomato juice intake by mass spectrometry-based metabolomics
Authors: Yannick Hövelmann
Annika Jagels
Robin Schmid
Florian Hübner
Hans-Ulrich Humpf
Publication date: 01-03-2020
Publisher: Springer Berlin Heidelberg
Keywords: Alkaloids
Alkaloids
Biomarkers
Published in: European Journal of Nutrition / Issue 2/2020
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-019-01935-4

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Identification of potential human urinary biomarkers for tomato juice intake by mass spectrometry-based metabolomics

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Purpose

Methods

Results

Conclusions

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