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European Journal of Nutrition

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01-06-2019 | Original Contribution

Dietary wheat amylase trypsin inhibitors exacerbate murine allergic airway inflammation

Authors: Victor F. Zevallos, Verena K. Raker, Joachim Maxeiner, Petra Scholtes, Kerstin Steinbrink, Detlef Schuppan

Published in: European Journal of Nutrition | Issue 4/2019

Abstract

Background

Wheat amylase trypsin inhibitors (ATI) are dietary non-gluten proteins that activate the toll-like receptor 4 on myeloid cells, promoting intestinal inflammation.

Aim of the study

We investigated the effects of dietary ATI on experimental allergic airway inflammation.

Methods

Mice on a gluten and ATI-free diet (GAFD), sensitized with PBS or ovalbumin (OVA) and challenged with OVA, were compared to mice on a commercial standard chow, a gluten diet naturally containing ~ 0.75% of protein as ATI (G+AD), a gluten diet containing ~ 0.19% of protein as ATI (G−AD) and a GAFD with 1% of protein as ATI (AD). Airway hyperreactivity (AHR), inflammation in bronchoalveolar lavage (BAL) and pulmonary tissue sections were analyzed. Allergic sensitization was assessed ex vivo via proliferation of OVA-stimulated splenocytes.

Results

Mice on a GAFD sensitized with PBS did not develop AHR after local provocation with methacholine. Mice on a GAFD or on a G−AD and sensitized with OVA developed milder AHR compared to mice fed a G+AD or an AD. The increased AHR was paralleled by increased BAL eosinophils, IL-5 and IL-13 production, and an enhanced ex vivo splenocyte activation in the ATI-fed groups.

Conclusions

Dietary ATI enhance allergic airway inflammation in OVA-challenged mice, while an ATI-free or ATI-reduced diet has a protective effect on AHR. Nutritional wheat ATI, activators of intestinal myeloid cells, may be clinically relevant adjuvants to allergic airway inflammation.

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Title: Dietary wheat amylase trypsin inhibitors exacerbate murine allergic airway inflammation
Authors: Victor F. Zevallos
Verena K. Raker
Joachim Maxeiner
Petra Scholtes
Kerstin Steinbrink
Detlef Schuppan
Publication date: 01-06-2019
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 4/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-018-1681-6

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Dietary wheat amylase trypsin inhibitors exacerbate murine allergic airway inflammation

Abstract

Background

Aim of the study

Methods

Results

Conclusions

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Abstract

Background

Aim of the study

Methods

Results

Conclusions

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