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Child's Nervous System
Published in: Child's Nervous System 8/2018

01-08-2018 | Original Paper

Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats

Authors: Xu-Gui Chen, Li-Hua Chen, Ru-Xiang Xu, Hong-Tian Zhang

Published in: Child's Nervous System | Issue 8/2018

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Abstract

Purpose

To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.

Methods

The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.

Results

From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (p < 0.05). Compared with the SCI group, tissue water content of the Mdivi-1 group and MP+Mdivi-1 group reduced obviously (p < 0.05), mitochondrial membrane potential (MMP) level and ATP content in the Mdivi-1 group and MP+Mdivi-1 group were both higher (p < 0.05). Meanwhile, three kinds of treatment all reduced apoptosis significantly, while MP plus Mdivi-1 exhibited the best inhibition effect on apoptosis (p < 0.05). The expression levels of Drp1, cytochrome c, and caspase-3 were all upregulated obviously; Mdivi-1 could inhibit Drp1 upregulation induced by SCI; for the upregulation of cytochrome c and caspase-3, the inhibition effect of Mdivi-1 approached MP. When MP combined with Mdivi-1, there was the best inhibition effect.

Conclusions

MP combined with Mdivi-1 may produce better neurological function recovery, through improving functional status of mitochondria and inhibiting lipid peroxidation in damaged tissue of SCI rats, and thus alleviating apoptosis.
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Metadata
Title
Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats
Authors
Xu-Gui Chen
Li-Hua Chen
Ru-Xiang Xu
Hong-Tian Zhang
Publication date
01-08-2018
Publisher
Springer Berlin Heidelberg
Published in
Child's Nervous System / Issue 8/2018
Print ISSN: 0256-7040
Electronic ISSN: 1433-0350
DOI
https://doi.org/10.1007/s00381-018-3792-z

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