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European Radiology
Published in: European Radiology 9/2014

01-09-2014 | Urogenital

T1-mapping for assessment of ischemia-induced acute kidney injury and prediction of chronic kidney disease in mice

Authors: Katja Hueper, Matti Peperhove, Song Rong, Jessica Gerstenberg, Michael Mengel, Martin Meier, Marcel Gutberlet, Susanne Tewes, Amelie Barrmeyer, Rongjun Chen, Herman Haller, Frank Wacker, Dagmar Hartung, Faikah Gueler

Published in: European Radiology | Issue 9/2014

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Abstract

Objectives

To investigate whether T1-mapping allows assessment of acute kidney injury (AKI) and prediction of chronic kidney disease (CKD) in mice.

Methods

AKI was induced in C57Bl/6N mice by clamping of the right renal pedicle for 35 min (moderate AKI, n = 26) or 45 min (severe AKI, n = 23). Sham animals served as controls (n = 9). Renal histology was assessed in the acute (day 1 + day 7; d1 + d7) and chronic phase (d28) after AKI. Furthermore, longitudinal MRI-examinations (prior to until d28 after surgery) were performed using a 7-Tesla magnet. T1-maps were calculated from a fat-saturated echoplanar inversion recovery sequence, and mean and relative T1-relaxation times were determined.

Results

Renal histology showed severe tubular injury at d1 + d7 in both AKI groups, whereas, at d28, only animals with prolonged 45-min ischemia showed persistent signs of AKI. Following both AKI severities T1-values significantly increased and peaked at d7. T1-times in the contralateral kidney without AKI remained stable. At d7 relative T1-values in the outer stripe of the outer medulla were significantly higher after severe than after moderate AKI (138 ± 2 % vs. 121 ± 3 %, p = 0.001). T1-elevation persisted until d28 only after severe AKI. Already at d7 T1 in the outer stripe of the outer medulla correlated with kidney volume loss indicating CKD (r = 0.83).

Conclusion

T1-mapping non-invasively detects AKI severity in mice and predicts further outcome.

Key Points

• Renal T1-relaxation times are increased after ischemia-induced acute kidney injury.
• Renal T1-values correlate with subsequent kidney volume loss.
• T1-mapping detects the severity of acute kidney injury and predicts further outcome.
Appendix
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Metadata
Title
T1-mapping for assessment of ischemia-induced acute kidney injury and prediction of chronic kidney disease in mice
Authors
Katja Hueper
Matti Peperhove
Song Rong
Jessica Gerstenberg
Michael Mengel
Martin Meier
Marcel Gutberlet
Susanne Tewes
Amelie Barrmeyer
Rongjun Chen
Herman Haller
Frank Wacker
Dagmar Hartung
Faikah Gueler
Publication date
01-09-2014
Publisher
Springer Berlin Heidelberg
Published in
European Radiology / Issue 9/2014
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI
https://doi.org/10.1007/s00330-014-3250-6

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