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Journal of Assisted Reproduction and Genetics

Published in:

01-07-2011 | Genetics

Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

Authors: Li Zhang, Ahmet Yilmaz, Ri-Cheng Chian, Weon-Young Son, Xiao Yun Zhang, Dewen Kong, Michael Dahan, Hannanel Holzer, Seang Lin Tan, Asangla Ao

Published in: Journal of Assisted Reproduction and Genetics | Issue 7/2011

Abstract

Purpose

To evaluate preimplantation genetic diagnosis (PGD) efficiency in thawed human embryos vitrified without biopsy.

Methods

In this retrospective clinical study, 21 PGD cycles were carried out using fresh and vitrified-thawed embryos collected from 21 patients.

Results

One hundred and ninety-nine embryos from patients with aneuploidy, single gene defects, or chromosomal translocations were vitrified at the cleavage stages; 93.5% of the embryos survived the vitrification procedure. Conclusive FISH results were obtained in 98.4% of the fresh and 99% of the frozen-thawed embryos screened for aneuploidy or chromosomal translocations. Conclusive PCR test results were obtained in 100% of the fresh and 93.9% of the frozen-thawed embryos screened for single gene defects. The overall clinical pregnancy rate per cycle was 57.1%. To date, 13 healthy children have been born.

Conclusion

Reliable genetic diagnosis can be performed in thawed human embryos vitrified without biopsy. However, further research is required to support this conclusion.

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Title: Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy
Authors: Li Zhang
Ahmet Yilmaz
Ri-Cheng Chian
Weon-Young Son
Xiao Yun Zhang
Dewen Kong
Michael Dahan
Hannanel Holzer
Seang Lin Tan
Asangla Ao
Publication date: 01-07-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 7/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9556-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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