Top

Journal of Assisted Reproduction and Genetics

Published in:

01-07-2011 | Fertility Preservation

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Authors: Jie Yan, Joao Suzuki, Xiao-Min Yu, Jie Qiao, Frederick W. K. Kan, Ri-Cheng Chian

Published in: Journal of Assisted Reproduction and Genetics | Issue 7/2011

Abstract

Purpose

To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming.

Methods

Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8–10 days, 90–92 days and 180–182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared.

Result(s)

The oocyte cryo-survival rate declined following cryo-storage duration for 180–182 days (90.4 ± 7.9%) compared to that of the other two groups (97.4 ± 3.0% and 98.0 ± 3.3%). The fertilization rate in the group of 180–182 days (66.6 ± 22.0%) was also significantly reduced (P < 0.05) compared with the groups of 8–10 days (92.2 ± 10.8%) and 90–92 days (94.7 ± 9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P < 0.05) following long cryo-storage duration (72.1 ± 8.2%, 25.2 ± 3.8% and 5.5 ± 13.6%, respectively).

Conclusion(s)

The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.

Chen C. Pregnancy after human oocyte cryopreservation. Lancet. 1986;1:884–6.PubMedCrossRef

Porcu E, Fabbri R, Seracchioli R, Ciotti PM, Magrini O, Flamigni C. Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril. 1997;68:724–6.PubMedCrossRef

Porcu E, Fabbri R, Ciotti P. Cycles of human oocyte crypreservation and intracytoplasmic sperm injection: results of 112 cycles. Fertil Steril. 1999;72:S2.

Winslow KL, Yang D, Blohm P. Oocyte cryopreservation: a three years follow up of sixteen births. Fertil Steril. 2001;76:S120.CrossRef

Yang DS, Blohm PL, Winslow KL. Oocyte donation using cryopreserved donor oocytes. Fertil Steril. 2002;78:S14.CrossRef

Boldt J, Cline D, McLaughlin D. Human oocyte cryopreservation as an adjunct to IVF-embryo transfer cycles. Hum Reprod. 2003;18:1250–5.PubMedCrossRef

Fosas N, Marina F, Torres PJ, Jove I, Martin P, Perez N, et al. The births of five Spanish babies from cryopreserved donated oocytes. Hum Reprod. 2003;18:1417–21.PubMedCrossRef

Borini A, Sciajno R, Bianchi V, Sereni E, Flamigni C, Coticchio G. Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration. Hum Reprod. 2006;21:512–7.PubMedCrossRef

Noyes N, Porcu E, Borini A. Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies. Reprod Biomed Online. 2009;18:769–76.PubMedCrossRef

10.

Chian RC, Huang JY, Tan SL, Lucena E, Saa A, Rojas A, et al. Obstetric and perinatal outcome in 200 infants conceived from vitrified oocytes. Reprod Biomed Online. 2008;16:608–10.PubMedCrossRef

11.

Cao YX, Xing Q, Li L, Cong L, Zhang ZG, Wei ZL, et al. Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification. Fertil Steril. 2009;92:1306–11.PubMedCrossRef

12.

Chen SU, Lien YR, Chen HF, Chang LJ, Tsai YY, Yang YS. Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI. Hum Reprod. 2005;20:1975–80.PubMedCrossRef

13.

Kuleshova L, Gianaroli L, Magli C, Ferraretti A, Trounson A. Birth following vitrification of a small number of human oocytes: case report. Hum Reprod. 1999;14:3077–9.PubMedCrossRef

14.

Yoon TK, Kim TJ, Park SE, Hong SW, Ko JJ, Chung HM, et al. Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril. 2003;79:1323–6.PubMedCrossRef

15.

Lucena E, Bernal DP, Lucena C, Rojas A, Moran A, Lucena A. Successful ongoing pregnancies after vitrification of oocytes. Fertil Steril. 2006;85:108–11.PubMedCrossRef

16.

Nagy ZP, Chang CC, Shapiro DB, Bernal DP, Elsner CW, Mitchell-Leef D, et al. Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil Steril. 2009;92:520–6.PubMedCrossRef

17.

Coticchio G, Bonu MA, Bianchi V, Flamigni C, Borini A. Criteria to assess human oocyte quality after cryopreservation. Reprod Biomed Online. 2005;11:421–7.PubMedCrossRef

18.

Parmegiani L, Garello C, Granella F, Guidetti D, Bernardi S, Cognigni GE, et al. Long-term cryostorage does not adversely affect the outcome of oocyte thawing cycles. Reprod Biomed Online. 2009;19:374–9.PubMedCrossRef

19.

Rienzi L, Romano S, Albricci L, Maggiulli R, Capalbo A, Baroni E, Colamaria S, Sapienza F, Ubaldi F. Embryo development of fresh ‘versus’ vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study. Hum Reprod. 2010;25:66–73.

20.

Cobo A, Meseguer M, Remohi J, Pellicer A. Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod. 2010;25:2239–46.

21.

Huang JY, Chen HY, Park JY, Tan SL, Chian RC. Comparison of spindle and chromosome configuration in in vitro- and in vivo-matured mouse oocytes after vitrification. Fertil Steril. 2008;90:1424–32.PubMedCrossRef

22.

Kimura Y, Yanagimachi R. Intracytoplasmic sperm injection in the mouse. Biol Reprod. 1995;52:709–20.PubMedCrossRef

23.

Handyside AH, Hunter S. A rapid procedure for visualising the inner cell mass and trophectoderm nuclei of mouse blastocysts in situ using polynucleotide-specific fluorochromes. J Exp Zool. 1984;231:429–34.PubMedCrossRef

24.

Yan J, Suzuki J, Yu X, Kan FW, Qiao J, Chian RC. Cryo-survival, fertilization and early embryonic development of vitrified oocytes derived from mice of different reproductive age. J Assist Reprod Genet. 2010;27:605–11.PubMedCrossRef

25.

Whittingham DG, Lyon MF, Glenister PH. Long-term storage of mouse embryos at −196 degrees C: the effect of background radiation. Genet Res. 1977;29:171–81.PubMedCrossRef

26.

Lyon MF, Whittingham DG, Glenister P. Long-term storage of frozen mouse embryos under increased background irradiation. Ciba Found Symp. 1977;52:273–90.

27.

Lyon MF, Glenister PH, Whittingham DG. Long-term viability of embryos stored under irradiation. In: Zeilmaker GH, Stuttgart FRG, editors. Frozen storage of laboratory animals. Stuttgard, New York: Gustav Fischer Verlag, 1981. p. 139–47.

28.

Glenister PH, Whittingham DG, Lyon MF. Further studies on the effect of radiation during the storage of frozen 8-cell mouse embryos at −196 degrees C. J Reprod Fertil. 1984;70:229–34.PubMedCrossRef

29.

Glenister PH, Lyon MF. Long-term storage of eight-cell mouse embryos at −196 degrees C. J In Vitro Fert Embryo Transf. 1986;3:20–7.PubMedCrossRef

30.

Glenister PH, Thornton CE. Cryoconservation—archiving for the future. Mamm Genome. 2000;11:565–71.PubMedCrossRef

31.

Riggs R, Mayer J, Dowling-Lacey D, Chi TF, Jones E, Oehninger S. Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos. Fertil Steril. 2010;93:109–15.PubMedCrossRef

32.

Cohen J, Inge KL, Wiker SR, Wright G, Fehilly CB, Turner Jr TG. Duration of storage of cryopreserved human embryos. J In Vitro Fert Embryo Transf. 1988;5:301–3.PubMedCrossRef

33.

Testart J, Lassalle B, Forman R, Gazengel A, Belaisch-Allart J, Hazout A, et al. Factors influencing the success rate of human embryo freezing in an in vitro fertilization and embryo transfer program. Fertil Steril. 1987;48:107–12.PubMed

34.

Quintans CJ, Donaldson MJ, Bertolino MV, Godoy H, Pasqualini RS. Birth of a healthy baby after transfer of embryos that were cryopreserved for 8.9 years. Fertil Steril. 2002;77:1074–6.PubMedCrossRef

35.

Ben-Ozer S, Vermesh M. Full term delivery following cryopreservation of human embryos for 7. 5 years. Hum Reprod. 1999;14:1650–2.PubMedCrossRef

36.

Go KJ, Corson SL, Batzer FR, Walters JL. Live birth from a zygote cryopreserved for 8 years. Hum Reprod. 1998;13:2970–1.PubMed

37.

Revel A, Safran A, Laufer N, Lewin A, Reubinov BE, Simon A. Twin delivery following 12 years of human embryo cryopreservation: case report. Hum Reprod. 2004;19:328–9.PubMedCrossRef

38.

Yang D, Brown SE, Nguyen K, Reddy V, Brubaker C, Winslow KL. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Fertil Steril. 2007;87:1469.e1–4.PubMedCrossRef

39.

Parmegiani L, Fabbri R, Cognigni GE, Bernardi S, Pocognoli P, Filicori M. Blastocyst formation, pregnancy, and birth derived from human oocytes cryopreserved for 5 years. Fertil Steril. 2008;5:2014.e7–10.

40.

McGee Jr HA, Martin WJ. Cryochemistry. Cryogenics. 1962;2:1–11.

41.

Gregory CR, Nuttall CW. Explosion risks in cryogenic liquids exposed to ionising radation. European organization for nuclear research. 1995;CERN AT/95-06 (DI):154–60.

42.

Brereton SJ. Explosion hazard in liquid nitrogen cooled fusion systems. Conference: 8 topical meeting on technology of fusion energy, Salt Lake City, UT, USA, 1988;EGG-M-88256; CONF-881031-55.

43.

Junk G, Svec JH. The presence of N3+, and N4+ in the mass spectra of molecular nitrogen. J Am Chem Soc. 1958;80:2908–9.CrossRef

44.

Rice FO. History of radical trapping. In: Bass AM, Broida HP, editors. Formation and trapping of free radicals. New York: Academic; 1960. p. 7.

Title: Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification
Authors: Jie Yan
Joao Suzuki
Xiao-Min Yu
Jie Qiao
Frederick W. K. Kan
Ri-Cheng Chian
Publication date: 01-07-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 7/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9563-3

At a glance: The STEP trials

Springer Medicine

Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification

Abstract

Purpose

Methods

Result(s)

Conclusion(s)

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Result(s)

Conclusion(s)

Please log in to get access to this content

Other articles of this Issue 7/2011

Frozen-thawed embryo transfer cycles: clinical outcomes of single and double blastocyst transfers

Anti-Mullerian hormone and antral follicle count as predictors for embryo/oocyte cryopreservation cycle outcomes in breast cancer patients stimulated with letrozole and follicle stimulating hormone

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Impact of reciprocal translocation t (18; 21) on male infertility and embryo development: lessons from an oocyte-donating ICSI cycle

Premature ovarian failure in nobox-deficient mice is caused by defects in somatic cell invasion and germ cell cyst breakdown

Treatment outcomes and quality-of-life assessment in a university-based fertility preservation program: Results of a registry of female cancer patients at 2 years