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Journal of Assisted Reproduction and Genetics

Published in:

01-06-2009 | ASSISTED REPRODUCTION

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Authors: Mojtaba Rezazadeh Valojerdi, Poopak Eftekhari-Yazdi, Leila Karimian, Fatemeh Hassani, Bahar Movaghar

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2009

Abstract

Purpose

The objective of this retrospective study was to evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate, post-warming embryo morphology and clinical outcomes.

Methods

The embryos of 305 patients at cleavage stages were cryopreserved either with vitrification (153 patients) or slow-freezing (152 patients) methods. After warming; the survival rate, post-warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups.

Result(s)

In the vitrification group versus slow freezing group, the survival rate (96.9% vs. 82.8%) and the post-warmed excellent morphology with all blastomeres intact (91.8% vs. 56.2%) were higher with an odds ratio of 6.607 (95% confidence interval; 4.184–10.434) and 8.769 (95% confidence interval; 6.460–11.904), respectively. In this group, the clinical pregnancy rate (40.5% vs. 21.4%) and the implantation rate (16.6% vs. 6.8%) were also higher with an odds ratio of 2.427 (95%confidence interval; 1.461–4.033) and 2.726 (95% confidence interval; 1.837–4.046), respectively.

Conclusion(s)

Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos. Vitrification provides a higher survival rate, minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.

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Title: Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos
Authors: Mojtaba Rezazadeh Valojerdi
Poopak Eftekhari-Yazdi
Leila Karimian
Fatemeh Hassani
Bahar Movaghar
Publication date: 01-06-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9318-6

At a glance: The STEP trials

Springer Medicine

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Abstract

Purpose

Methods

Result(s)

Conclusion(s)

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Result(s)

Conclusion(s)

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