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Archives of Virology
Published in: Archives of Virology 9/2018

01-09-2018 | Brief Report

Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses

Authors: Fan Yang, Bin Chen, Fumin Liu, Xiuming Peng, Tao Sun, Hangping Yao, Haibo Wu, Nanping Wu

Published in: Archives of Virology | Issue 9/2018

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Abstract

H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR primers were designed based on gene regions encoding conserved domains of the nucleoprotein and H10 hemagglutinin. The developed multiplex real-time RT-PCR assay displayed high specificity, repeatability, and a detection limit of 10 copies per reaction. This diagnostic method could prove valuable for the rapid detection of H10 subtype AIVs in China.
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Metadata
Title
Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses
Authors
Fan Yang
Bin Chen
Fumin Liu
Xiuming Peng
Tao Sun
Hangping Yao
Haibo Wu
Nanping Wu
Publication date
01-09-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3889-4

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