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01-06-2018 | Brief Report

Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses

Authors: Fan Yang, Haibo Wu, Fumin Liu, Xiangyun Lu, Xiuming Peng, Nanping Wu

Published in: Archives of Virology | Issue 6/2018

Abstract

The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.

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Title: Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses
Authors: Fan Yang
Haibo Wu
Fumin Liu
Xiangyun Lu
Xiuming Peng
Nanping Wu
Publication date: 01-06-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 6/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3773-2

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