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BMC Complementary Medicine and Therapies

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Open Access 01-12-2019 | Leishmania | Research article

Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss

Authors: Tanzeel Zohra, Muhammad Ovais, Ali Talha Khalil, Muhammad Qasim, Muhammad Ayaz, Zabta Khan Shinwari, Sajjad Ahmad, Mohammad Zahoor

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Abstract

Background

Plants represent an intricate and innovative source for the discovery of novel therapeutic remedies for the management of various ailments. The current study has been aimed to validate the therapeutic potential of ethnomedicinally significant plant Atriplex lasiantha Boiss.

Methods

The polarity based extraction process was carried out using fourteen solvents to figure out best extraction solvent and bioactive fractions. Total phenolic-flavonoids contents were quantified colorimetrically and polyphenolics were measured using HPLC-DAD analysis. Moreover, the test samples were tested against several diseases targets following various assays including free radicals scavenging, antibacterial, antifungal, cytotoxic and antileishmanial assay.

Results

Among the solvent fractions, maximum yield was obtained with methanol-water extract i.e., 11 ± 0.49%. Maximum quantity of gallic acid equivalent phenolic content and quercetin equivalent flavonoid content were quantified in methanol-ethyl acetate extract of A. lasiantha. Significant quantity of rutin i.e., 0.3 μg/mg was quantified by HPLC analysis. The methanol-ethyl acetate extract of A. lasiantha exhibited maximum total antioxidant and total reducing power with 64.8 ± 1.16 AAE/mg extract respectively, while showing 59.8 ± 1.07% free radical scavenging potential. A significant antibacterial potential was exhibited by acetone-distilled water extract of A. lasiantha with 11 ± 0.65 mm zone of inhibition against B. subtilis. Considerable antifungal activity was exhibited by ethyl acetate-n-hexane extract of aerial part of A. lasiantha with 14 ± 1.94 mm zone of inhibition against A. fumigatus. Highest percentage of α-amylase inhibition (41.8 ± 1.09%) was observed in ethyl acetate-n-hexane extract. Methanol-acetone extract of A. lasiantha demonstrated significant inhibition of hyphae formation with 11 ± 0.49 mm bald zone of inhibition. Significant in-vitro cytotoxicity against Hep G2 cell line has been exhibited by methanol-chloroforms extract of A. lasiantha.

Conclusion

The current study reveals the prospective potential of Atriplex lasiantha Boiss. for the discovery of biologically active compounds through bioassay guided isolation against various diseases.

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Title: Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss
Authors: Tanzeel Zohra
Muhammad Ovais
Ali Talha Khalil
Muhammad Qasim
Muhammad Ayaz
Zabta Khan Shinwari
Sajjad Ahmad
Mohammad Zahoor
Publication date: 01-12-2019
Publisher: BioMed Central
Keywords: Leishmania
Leishmania
Published in: BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-018-2416-1

At a glance: The STEP trials

Springer Medicine

Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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