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Archives of Virology
Published in: Archives of Virology 5/2013

01-05-2013 | Brief Report

Identification of a novel bluetongue virus 1-specific B-cell epitope using a monoclonal antibody against the VP2 protein

Authors: P. Wei, E. C. Sun, N. H. Liu, T. Yang, Q. Y. Xu, J. Zhao, Y. L. Qin, Y. F. Feng, J. P. Li, W. S. Wang, C. Y. Zhang, D. L. Wu

Published in: Archives of Virology | Issue 5/2013

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Abstract

Bluetongue virus (BTV) VP2 is an important antigenic protein that can be used for the differential diagnosis of different BTV serotypes. Here, we generated a serotype-specific monoclonal antibody (mab) against BTV1. A series of peptides synthesized based on the amino acid sequence of BTV1 VP2 were screened to define 115AQPLKVGL122 as the minimal linear peptide epitope recognized by mab 4B6. Using an immunofluorescence assay (IFA), we found that mab 4B6 reacted strongly with BTV1, but did not react with other BTV serotypes (BTV2-24). The 4B6 will serve as a novel reagent in the development of diagnostic tests for BTV1 infection.
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Metadata
Title
Identification of a novel bluetongue virus 1-specific B-cell epitope using a monoclonal antibody against the VP2 protein
Authors
P. Wei
E. C. Sun
N. H. Liu
T. Yang
Q. Y. Xu
J. Zhao
Y. L. Qin
Y. F. Feng
J. P. Li
W. S. Wang
C. Y. Zhang
D. L. Wu
Publication date
01-05-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 5/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1590-6

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