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01-05-2013 | Original Article

Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography

Authors: L. Huhti, K. Tamminen, T. Vesikari, V. Blazevic

Published in: Archives of Virology | Issue 5/2013

Abstract

Recombinant baculovirus (BV) expression systems are widely applied in the production of viral capsid proteins and virus-like particles (VLPs) for use as immunogens and vaccine candidates. Traditional density gradient purification of VLPs does not enable complete elimination of BV-derived impurities, including live viruses, envelope glycoprotein gp64 and baculoviral DNA. We used an additional purification system based on ionic strength to purify norovirus (NoV) GII-4 capsid-derived VLPs. The anion exchange chromatography purification led to highly purified VLPs free from BV impurities with intact morphology. In addition, highly purified VLPs induced strong NoV-specific antibody responses in BALB/c mice. Here, we describe a method for NoV VLP purification and several methods for determining their purity, including quantitative PCR for BV DNA detection.

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Title: Characterization and immunogenicity of norovirus capsid-derived virus-like particles purified by anion exchange chromatography
Authors: L. Huhti
K. Tamminen
T. Vesikari
V. Blazevic
Publication date: 01-05-2013
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 5/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-012-1565-7

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