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Cardiovascular Toxicology
Published in: Cardiovascular Toxicology 5/2019

01-10-2019 | Hypertension

Carbon Monoxide Attenuates High Salt-Induced Hypertension While Reducing Pro-inflammatory Cytokines and Oxidative Stress in the Paraventricular Nucleus

Authors: Dong-Dong Zhang, Yan-Feng Liang, Jie Qi, Kai B. Kang, Xiao-Jing Yu, Hong-Li Gao, Kai-Li Liu, Yan-Mei Chen, Xiao-Lian Shi, Guo-Rui Xin, Li-Yan Fu, Yu-Ming Kang, Wei Cui

Published in: Cardiovascular Toxicology | Issue 5/2019

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Abstract

Carbon monoxide (CO) presents anti-inflammatory and antioxidant activities as a new gaseous neuromessenger produced by heme oxygenase-1 (HO-1) in the body. High salt-induced hypertension is relevant to the levels of pro-inflammatory cytokines (PICs) and oxidative stress in the hypothalamic paraventricular nucleus (PVN). We explored whether CO in PVN can attenuate high salt-induced hypertension by regulating PICs or oxidative stress. Male Dahl Salt-Sensitive rats were fed high-salt (8% NaCl) or normal-salt (0.3% NaCl) diet for 4 weeks. CORM-2, ZnPP IX, or vehicle was microinjected into bilateral PVN for 6 weeks. High-salt diet increased the levels of MAP, plasma norepinephrine (NE), reactive oxygen species (ROS), and the expressions of COX2, IL-1β, IL-6, NOX2, and NOX4 significantly in PVN (p < 0.05), but decreased the expressions of HO-1 and Cu/Zn-SOD in PVN (p < 0.05). Salt increased sympathetic activity as measured by circulating norepinephrine, and increased the ratio of basal RSNA to max RSNA, in part by decreasing max RSNA. PVN microinjection of CORM-2 decreased the levels of MAP, NE, RSNA, ROS and the expressions of COX2, IL-1β, IL-6, NOX2, NOX4 significantly in PVN of hypertensive rat (p < 0.05), but increased the expressions of HO-1 and Cu/Zn-SOD significantly (p < 0.05), which were all opposite to the effects of ZnPP IX microinjected in PVN (p < 0.05). We concluded that exogenous or endogenous CO attenuates high salt-induced hypertension by regulating PICs and oxidative stress in PVN.
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Metadata
Title
Carbon Monoxide Attenuates High Salt-Induced Hypertension While Reducing Pro-inflammatory Cytokines and Oxidative Stress in the Paraventricular Nucleus
Authors
Dong-Dong Zhang
Yan-Feng Liang
Jie Qi
Kai B. Kang
Xiao-Jing Yu
Hong-Li Gao
Kai-Li Liu
Yan-Mei Chen
Xiao-Lian Shi
Guo-Rui Xin
Li-Yan Fu
Yu-Ming Kang
Wei Cui
Publication date
01-10-2019
Publisher
Springer US
Published in
Cardiovascular Toxicology / Issue 5/2019
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-019-09517-w

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