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Journal of Assisted Reproduction and Genetics

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01-04-2013 | Genetics

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

Authors: Mohamed Hassen Chelli, Fatma Ferfouri, Florence Boitrelle, Martine Albert, Denise Molina-Gomes, Jacqueline Selva, François Vialard

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2013

Abstract

Problem

This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation.

Method of study

We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as “ICSI-like”, and (iii) with MSOME.

Results

The balanced translocation rates did not differ significantly (p = 0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p = 0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %).

Conclusion

For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.

Antinori M, Licata E, Dani G, Cerusico F, Versaci C, d’Angelo D, et al. Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed Online. 2008;16(6):835–41.PubMedCrossRef

Auger J, Eustache F, Ducot B, Blandin T, Daudin M, Diaz I, et al. Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories. Hum Reprod. 2000;15(11):2360–8.PubMedCrossRef

Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, et al. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80(6):1413–9.PubMedCrossRef

Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl. 2002;23(1):1–8.PubMed

Benet J, Oliver-Bonet M, Cifuentes P, Templado C, Navarro J. Segregation of chromosomes in sperm of reciprocal translocation carriers: a review. Cytogenet Genome Res. 2005;111(3–4):281–90.PubMedCrossRef

Berkovitz A, Eltes F, Ellenbogen A, Peer S, Feldberg D, Bartoov B. Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome? Hum Reprod. 2006;21(7):1787–90.PubMedCrossRef

Berkovitz A, Eltes F, Yaari S, Katz N, Barr I, Fishman A, et al. The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod. 2005;20(1):185–90.PubMedCrossRef

Boitrelle F, Ferfouri F, Petit JM, Segretain D, Tourain C, Bergere M, et al. Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod. 2011;26(7):1650–8.PubMedCrossRef

Calogero AE, Burrello N, De Palma A, Barone N, D’Agata R, Vicari E. Sperm aneuploidy in infertile men. Reprod Biomed Online. 2003;6(3):310–7.PubMedCrossRef

10.

Cassuto NG, Le Foll N, Chantot-Bastaraud S, Balet R, Bouret D, Rouen A, et al. Sperm fluorescence in situ hybridization study in nine men carrying a Robertsonian or a reciprocal translocation: relationship between segregation modes and high-magnification sperm morphology examination. Fertil Steril. 2011;96(4):826–32.PubMedCrossRef

11.

Chelli MH, Albert M, Ray PF, Guthauser B, Izard V, Hammoud I, et al. Can intracytoplasmic morphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm head syndrome? Fertil Steril. 2009;93(4):1347 e1341–1345.

12.

Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231–45.PubMedCrossRef

13.

Estop AM, Cieply K, Munne S, Surti U, Wakim A, Feingold E. Is there an interchromosomal effect in reciprocal translocation carriers? Sperm FISH studies. Hum Genet. 2000;106(5):517–24.PubMedCrossRef

14.

Ferfouri F, Selva J, Boitrelle F, Gomes DM, Torre A, Albert M, et al. The chromosomal risk in sperm from heterozygous Robertsonian translocation carriers is related to the sperm count and the translocation type. Fertil Steril. 2011;96(6):1337–43.PubMedCrossRef

15.

Frainais C, Vialard F, Rougier N, Aegerther P, Damond F, Ayel JP, et al. Impact of freezing/thawing technique on sperm DNA integrity in HIV-1 patients. J Assist Reprod Genet. 2010;27(7):415–21.PubMedCrossRef

16.

Franco Jr JG, Baruffi RL, Mauri AL, Petersen CG, Oliveira JB, Vagnini L. Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod Biomed Online. 2008;17(1):42–5.PubMedCrossRef

17.

Franco JG, Mauri AL, Petersen CG, Massaro FC, Silva LF, Felipe V, Cavagna M, Pontes A, Baruffi RL, Oliveira JB, Vagnini LD (2011) Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl.

18.

Garolla A, Fortini D, Menegazzo M, De Toni L, Nicoletti V, Moretti A, et al. High-power microscopy for selecting spermatozoa for ICSI by physiological status. Reprod Biomed Online. 2008;17(5):610–6.PubMedCrossRef

19.

Kirkpatrick G, Ma S. Meiotic segregation and interchromosomal effects in a rare (1:2:10) complex chromosomal rearrangement. J Assist Reprod Genet. 2012;29(1):77–81.PubMedCrossRef

20.

Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Veeck LL, et al. New method of evaluating sperm morphology with predictive value for human in vitro fertilization. Urology. 1987;30(3):248–51.PubMedCrossRef

21.

Lindenbaum RH, Hulten M, McDermott A, Seabright M. The prevalence of translocations in parents of children with regular trisomy 21: a possible interchromosomal effect? J Med Genet. 1985;22(1):24–8.PubMedCrossRef

22.

Morel F, Douet-Guilbert N, Le Bris MJ, Herry A, Amice V, Amice J, et al. Meiotic segregation of translocations during male gametogenesis. Int J Androl. 2004;27(4):200–12.PubMedCrossRef

23.

Moretti E, Pascarelli NA, Giannerini V, Geminiani M, Anichini C, Collodel G. 18, X, Y aneuploidies and transmission electron microscopy studies in spermatozoa from five carriers of different reciprocal translocations. Asian J Androl. 2009;11(3):325–32.PubMedCrossRef

24.

Palermo G, Hu J, Rienzi L, Maggiulli R, Takeuchi T, Yoshida A, et al. Thoughts on IMSI. New York: Springer; 2011. p. 277–89.

25.

Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340(8810):17–8.PubMedCrossRef

26.

Pellestor F, Puechberty J, Weise A, Lefort G, Anahory T, Liehr T, Sarda P (2011) Meiotic segregation of complex reciprocal translocations: direct analysis of the spermatozoa of a t(5;13;14) carrier. Fertil Steril.

27.

Perdrix A, Travers A, Chelli MH, Escalier D, Do Rego JL, Milazzo JP, et al. Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles. Hum Reprod. 2011;26(1):47–58.PubMedCrossRef

28.

Perrin A, Basinko A, Douet-Guilbert N, Gueganic N, Le Bris MJ, Amice V, et al. Aneuploidy and DNA fragmentation in sperm of carriers of a constitutional chromosomal abnormality. Cytogenet Genome Res. 2011;133(2–4):100–6.PubMedCrossRef

29.

Rousseaux S, Chevret E, Monteil M, Cozzi J, Pelletier R, Devillard F, et al. Meiotic segregation in males heterozygote for reciprocal translocations: analysis of sperm nuclei by two and three colour fluorescence in situ hybridization. Cytogenet Cell Genet. 1995;71(3):240–6.PubMedCrossRef

30.

Shi Q, Martin RH. Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities, and in infertile men. Reproduction. 2001;121(5):655–66.PubMedCrossRef

31.

Vanderzwalmen P, Hiemer A, Rubner P, Bach M, Neyer A, Stecher A, et al. Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online. 2008;17(5):617–27.PubMedCrossRef

32.

Watanabe S, Tanaka A, Fujii S, Mizunuma H, Fukui A, Fukuhara R, et al. An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay. Hum Reprod. 2011;26(5):978–86.PubMedCrossRef

33.

Wilding M, Coppola G, di Matteo L, Palagiano A, Fusco E, Dale B. Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet. 2011;28(3):253–62.PubMedCrossRef

34.

World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Cambridge: Cambridge University Press; 2009.

Title: High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations
Authors: Mohamed Hassen Chelli
Fatma Ferfouri
Florence Boitrelle
Martine Albert
Denise Molina-Gomes
Jacqueline Selva
François Vialard
Publication date: 01-04-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 4/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-9959-3

At a glance: The STEP trials

Springer Medicine

High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

Abstract

Problem

Method of study

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Problem

Method of study

Results

Conclusion

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