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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 4/2013

01-04-2013 | Gamete Biology

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Authors: Yung-Chieh Tasi, Hsin-Chih Albert Chao, Chia-Ling Chung, Xiu-Ying Liu, Ying-Ming Lin, Pao-Chi Liao, Hsien-An Pan, Han-Sun Chiang, Pao-Lin Kuo, Ying-Hung Lin

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2013

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Abstract

Purpose

Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized.

Methods

Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (< 50 %) and lower motility (< 20 %).

Results

Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P < 0.0001 and P < 0.05, respectively).

Conclusions

Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.
Appendix
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Metadata
Title
Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker
Authors
Yung-Chieh Tasi
Hsin-Chih Albert Chao
Chia-Ling Chung
Xiu-Ying Liu
Ying-Ming Lin
Pao-Chi Liao
Hsien-An Pan
Han-Sun Chiang
Pao-Lin Kuo
Ying-Hung Lin
Publication date
01-04-2013
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 4/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-9954-8

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