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Journal of Assisted Reproduction and Genetics

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Open Access 01-04-2013 | Embryo Biology

The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification

Authors: Ying-qi Nong, Feng-hua Liu, Ye Chen, Fang Wang

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2013

Abstract

Purpose

To determine the role of aquaporin 3 (AQP3) isoform in early embryonic development and protecting embryos subjected to freeze–thawing for assisted reproduction, we investigated the expression and distribution of AQP3 in mouse embryos at different developmental stages before and after vitrification.

Methods

Eight-cell embryos, morulae, and blastocysts were obtained from female mice that had been superovulated by controlled ovarian hyperstimulation. Immunofluorescence staining, laser confocal microscopy, and Western blot were used to determine the expression and distribution of AQP3 in preimplantation mouse embryos before and after vitrification.

Results

AQP3 was expressed at the 8-cell to blastocyst stage before and after vitrification. The expression and distribution of AQP3 was developmentally regulated at the 8-cell to blastocyst stage. The expression of AQP3 was significantly decreased in 8-cell embryos and early blastocysts after vitrification. However, at the morulae stage, the expression of AQP3 was increased after vitrification.

Conclusions

The developmental and vitrification-dependent changes in AQP3 expression and distribution suggest that this transmembrane channel might regulate mouse embryo development and contribute to the protective response during vitrification.

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Title: The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification
Authors: Ying-qi Nong
Feng-hua Liu
Ye Chen
Fang Wang
Publication date: 01-04-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 4/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-9965-5

At a glance: The STEP trials

Springer Medicine

The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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