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01-12-2020 | Breast Cancer | Primary research

EO771, the first luminal B mammary cancer cell line from C57BL/6 mice

Authors: Augustin Le Naour, Yvonne Koffi, Mariane Diab, Delphine Le Guennec, Stéphanie Rougé, Sahar Aldekwer, Nicolas Goncalves-Mendes, Jérémie Talvas, Marie-Chantal Farges, Florence Caldefie-Chezet, Marie-Paule Vasson, Adrien Rossary

Published in: Cancer Cell International | Issue 1/2020

Abstract

Background

Despite decades of therapeutic trials, effective diagnosis, many drugs available and numerous studies on breast cancer, it remains the deadliest cancer in women. In order to choose the most appropriate treatment and to understand the prognosis of the patients, breast cancer is divided into different subtypes using a molecular classification. Just as there remains a need to discover new effective therapies, models to test them are also required.

Methods

The EO771 (also named E0771 or EO 771) murine mammary cancer cell line was originally isolated from a spontaneous tumour in C57BL/6 mouse. Although frequently used, this cell line remains poorly characterized. Therefore, the EO771 phenotype was investigated. The phenotype was compared to that of MCF-7 cells, known to be of luminal A subtype and to express estrogen receptors, as well as MDA-MB-231 cells, which are triple negative. Their sensitivity to hormonal treatment was evaluated by viability tests.

Results

The EO771 were estrogen receptor α negative, estrogen receptor β positive, progesterone receptor positive and ErbB2 positive. This phenotype was associated with a sensitivity to anti-estrogen treatments such as tamoxifen, 4-hydroxy-tamoxifen, endoxifen and fulvestrant.

Conclusions

On account of the numerous results published with the EO771 cell line, it is important to know its classification, to facilitate comparisons with corresponding types of tumours in patients. Transcriptomic and protein analysis of the EO771 cell line classified it within the luminal B subtype. Luminal B cancers correspond to one of the subtypes most frequently encountered in patients and associated with a poor prognosis.

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Title: EO771, the first luminal B mammary cancer cell line from C57BL/6 mice
Authors: Augustin Le Naour
Yvonne Koffi
Mariane Diab
Delphine Le Guennec
Stéphanie Rougé
Sahar Aldekwer
Nicolas Goncalves-Mendes
Jérémie Talvas
Marie-Chantal Farges
Florence Caldefie-Chezet
Marie-Paule Vasson
Adrien Rossary
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Breast Cancer
Breast Cancer
Tamoxifen
Estrogens
Estradiol
Fulvestrant
Published in: Cancer Cell International / Issue 1/2020
Electronic ISSN: 1475-2867
DOI: https://doi.org/10.1186/s12935-020-01418-1

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Abstract

Background

Methods

Results

Conclusions

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