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Comparative Clinical Pathology

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01-02-2019 | Xanthine Alkaloid | Original Article

Protective effects of curcumin on ischemic reperfusion of rat retina

Authors: Arash Esfandiari, Farid Hashemi

Published in: Comparative Clinical Pathology | Issue 1/2019

Abstract

Background:

Ischemic reperfusion is an optic neuropathy in which photoreceptor cells may die through an apoptotic process. Free radicals are involved in the apoptotic process of ischemic reperfusion disorders. Previous research indicates that curcumin is an antioxidant found in plants which reduces neurodegenerative changes in aged animals.

Aims:

The purpose of this research was to survey whether curcumin has preventive effects on ischemic reperfusion of the retina.

Methods:

Twenty male Wistar rats were grouped into four (n = 5): 1, control group; 2, ischemic reperfusion group; 3, experimental group II: received 0.01% curcumin in diet (low dose) for 2 days before ischemic reperfusion; 4, experimental group III: received 0.25% curcumin in diet (high dose) for 2 days before ischemic reperfusion. The eye was enucleated and the retina separated. Routine tissue processing in transmission electron microscopy was done.

Results:

Results showed that ischemic reperfusion created major signs of injury in the photoreceptor layer such as disappearance of the outer segment, cell swelling, and karyolitic and pyknotic nuclei, but cell swelling and karyolitic nuclei were not seen after receiving a low dose of curcumin. Also, the photoreceptor layer appeared normal after receiving a high dose of curcumin, but decreased vacuolization and dark and inactive nuclei were obvious.

Conclusions:

This study concluded that a high dose of curcumin confers a more preventive effect on retinal injury by ischemic reperfusion than a low dose.

Agardh CD, Gustavsson C, Hagert P, Nilsson M, Agardh E (2006) Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion. Metab 55:892–898. https://doi.org/10.1016/j.metabol.2006.02.016 CrossRef

Aldebasi YH, Aly SM, Rahmani AH (2013) Therapeutic implications of curcumin in the prevention of diabetic retinopathy via modulation of anti-oxidant activity and genetic pathway. Int J Physiol Pathophysiol Pharmacol 5:194–202PubMedPubMedCentral

Aliabadi A, Esfandiari A, Rajaei R (2016) Transmission electron microscopy of the retina following thyroidectomy in rabbit. Comp Clin Pathol 25(2):265–269. https://doi.org/10.1007/s00580-015-2176-y CrossRef

Anna K, Mammis JA, Savitz SI et al (2002) Erythropoietin administration protects retinal neurons from acute ischemia-reperfusion injury. PNAS 99(16):10659–10664. https://doi.org/10.1073/pnas.152321399 CrossRef

Baudouin C, Pisella PJ, Ettaiche M, Goldschild M, Becquet F, Gastaud P (1999) Effects of EGb761 and superoxide dismutase in an experimental model of retinopathy generated by intravitreal production of superoxide anion radical. Graefes Arch Clin Exp Ophthalmol 237:58–66CrossRefPubMed

Bonne C, Muller A, Villain M (1998) Free radicals in retinal ischemia. Gen Pharmacol 30:275–280CrossRefPubMed

Coyle JT, Puttfarcken P (1993) Oxidative stress, glutamate and neurodegenerative disorders. Science 262:689–695CrossRefPubMed

Dilsiz N, Sahaboglu A, Yildiz MZ, Reichenbach A (2006) Protective effects of various antioxidants during ischemia-reperfusion in the rat retina. Graefes Arch Clin Exp Ophthalmol 244:627–633. https://doi.org/10.1007/s00417-005-0084-6 CrossRefPubMed

Esfandiari A, Yousofi AR, Dehghan A, Safavi A (2009) Effect of intermittent light on photoreceptor cells in the rabbit retina. Jpn J Ophthalmol 53:635–639. https://doi.org/10.1007/s10384-009-0721-4 CrossRefPubMed

Flammer J, Orgül S (1998) Optic nerve blood-flow abnormalities in glaucoma. Prog Retin Eye Res 17:267–289CrossRefPubMed

Fox NE, van Kuijk FJ (1998) Immunohistochemical localization of xanthine oxidase in human retina. Free Radic Biol Med 24:900–905CrossRefPubMed

Guo L, Moss SE, Alexander RA, Ali RR, Fitzke FW, Cordeiro MF (2005) Retinal ganglion cell apoptosis in glaucoma is related to intraocular pressure and IOP induced effects on extracellular matrix. Invest Ophthalmol Vis Sci 46:175–182. https://doi.org/10.1167/iovs.04-0832 CrossRefPubMedPubMedCentral

Hardy P, Beauchamp M, Sennlaub F et al (2005) New insights into the retinal circulation: inflammatory lipid mediators in ischemic retinopathy. Prostaglandins Leukot Essent Fatty Acids 72:301–325. https://doi.org/10.1016/j.plefa.2005.02.004 CrossRefPubMed

Jo N, Wu GS, Rao NA (2003) Up regulation of chemokine expression in the retinal vasculature in ischemia-reperfusion injury. Invest Ophthalmol Vis Sci 44:4054–4060CrossRefPubMed

Leinonen JS, Ahonen JP, Lonnrot K et al (2000) Low plasma antioxidant activity is associated with high lesion volume and neurological impairment in stroke. Stroke 31:33–39CrossRefPubMed

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

Mallick IH, Yang W, Winslet MC, Seifalian AM (2004) Ischemia-reperfusion injury of the intestine and protective strategies against injury. Dig Dis Sci 49:1359–1377CrossRefPubMed

Moreno MC, Campanelli J, Sande P, Sánez DA et al (2004) Retinal oxidative stress induced by high intraocullar pressure. Free Radic Biol Med 37(6):803–812CrossRefPubMed

Nakashima M, Niwa M, Iwai T, Uematsu T (1999) Involvement of free radicals in cerebral vascular reperfusion injury evaluated in a transient focal cerebral ischemia model of rat. Free Radic Biol Med 26:722–729CrossRefPubMed

Okamoto T, Yamagishi S, Inagaki Y, Amano S et al (2002) Angiogenesis induced by advanced glycation and products and prevention by cerivastatin. FASEB 16:1928–1930. https://doi.org/10.1096/fj.02-0030fje CrossRef

Osawa T, Kato Y (2005) Protective role of antioxidative food factors in oxidative stress caused by hyperglycemia. Ann N Y Acad Sci 1043:440–451. https://doi.org/10.1196/annals.1333.050 CrossRefPubMed

Osborne NN, Casson RJ, Wood JP, Chidlow G et al (2004) Retinal ischemia: mechanisms of damage and potential therapeutic strategies. Prog Retin Eye Res 23:91–147. https://doi.org/10.1016/j.preteyeres.2003.r12.001 CrossRefPubMed

Oz O, Gürelik G, Akyürek N, Cinel L et al (2005) A short duration transient ischemia induces apoptosis in retinal layers: an experimental study in rabbits. Eur J Ophthalmol 15:233–238CrossRefPubMed

Patricia VT (2008) The Calam/Acmal Standards of Veterinary Care and laboratory animal welfare. Can Vet J 49:86–88

Peddada KV, Brown A, Verma V, Nebbioso M (2018) Therapeutic potential of curcumin in major retinal pathologies. Int Ophthalmol 2018. https://doi.org/10.1007/s10792-018-0845-y

Rosenbaum DM, Rosenbaum PS, Singh M, Gupta G, Gupta H, Li B, Roth S (2001) Functional and morphologic comparison of two methods to produce transient retinal ischemia in the rat. J Neuroophthalmol 21:62–68CrossRefPubMed

Sakamoto K, Yonoki Y, Kuwagata M, Saito M, Nakahara T, Ishii K (2004) Histological protection against ischemia-reperfusion injury by early ischemic preconditioning in rat retina. Brain Res 1015:154–160. https://doi.org/10.1016/j.brainres.2004.04.074 CrossRefPubMed

Sanchez RN, Chan CK, Garg S, Kwong JMK, Wong MJ, Sadun AA, Lam TT (2003) Interleukin-6 in retinal ischemia reperfusion injury in rats. Invest Ophthalmol Vis Sci 44:4006–4011CrossRefPubMed

Srinivasan M (1972) Effect of curcumin on blood sugar as seen in a diabetic subject. Indian J Med Sci 26:269–270PubMed

Strasser EM, Wessner B, Manhart N, Roth E (2005) The relationship between the anti-inflammatory effects of curcumin and cellular gluthatione content in myelomonocytic cells. Biochem Pharmacol 70:552–559. https://doi.org/10.1016/j.bcp.2005.05.030 CrossRefPubMed

Sugawara T, Chan PH (2003) Reactive oxygen radicals and pathogenesis of neuronal death after cerebral ischemia. Antioxid Redox Signal 5:597–607. https://doi.org/10.1089/152308603770310266 CrossRefPubMed

Szabo ME, Droy-Lefaix MT, Doly M, Carré C, Braquet P (1991) Ischemia and reperfusion induced histologic changes in the rat retina. Demonstration of a free radical-mediated mechanism. Invest Ophthalmol Vis Sci 32:1471–1478PubMed

Thaakur S, Sravanthi R (2010) Neuroprotective effect of Spirulina in cerebral ischemia–reperfusion injury in rats. J Neural Transm 117:1083–1091. https://doi.org/10.1007/s00702-010-0440-5 CrossRefPubMed

Wang Y, Chang CF, Chou J, Chen HL, Deng X, Harvey BK, Cadet JL, Bickford PC (2005) Dietary supplementation with blueberries, spinach, or spirulina reduces ischemic brain damage. Exp Neurol 193:75–84. https://doi.org/10.1016/j.expneurol.2004.12.014 CrossRefPubMed

Wang L, Li C, Guo H, Kern TS, Huang K, Zheng L (2011) Curcumin inhibits neuronal and vascular degeneration in retina after ischemia and reperfusion injury. PLoS One 6:e23194. https://doi.org/10.1371/journal.pone.0023194 CrossRefPubMedPubMedCentral

Warner D, Sheng H, Batinic-Haberle I (2004) Oxidants, antioxidants and the ischemic brain. J Exp Biol 207:3221–3231. https://doi.org/10.1242/jeb.01022 CrossRefPubMed

Yang GY, Pang L, Ge HL, Tan M, Ye W, Liu XH, Huang FP, Wu DC, Che XM, Song Y, Wen R, Sun Y (2001) Attenuation of ischemia-induced mouse brain injury by SAG, a redox-inducible antioxidant protein. J Cereb Blood Flow Metab 21:722–733. https://doi.org/10.1097/00004647-200106000-00010 CrossRefPubMed

Zheng L, Gong B, Hatala DA, Kern TS (2007) Retinal ischemia and reperfusion causes capillary degeneration: similarities to diabetes. Invest Ophthalmol Vis Sci 48:361–367. https://doi.org/10.1167/iovs.06-0510 CrossRefPubMed

Title: Protective effects of curcumin on ischemic reperfusion of rat retina
Authors: Arash Esfandiari
Farid Hashemi
Publication date: 01-02-2019
Publisher: Springer London
Keyword: Xanthine Alkaloid
Published in: Comparative Clinical Pathology / Issue 1/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-019-02894-2

At a glance: The STEP trials

Springer Medicine

Protective effects of curcumin on ischemic reperfusion of rat retina

Abstract

Background:

Aims:

Methods:

Results:

Conclusions:

At a glance: The STEP trials

Springer Medicine

Abstract

Background:

Aims:

Methods:

Results:

Conclusions:

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