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Journal of Assisted Reproduction and Genetics

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01-01-2012 | Gamete Biology

Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion

Authors: Forouzan Absalan, Alireza Ghannadi, Marjaneh Kazerooni, Rostami Parifar, Fatemeh Jamalzadeh, Sahar Amiri

Published in: Journal of Assisted Reproduction and Genetics | Issue 1/2012

Abstract

Objective

To evaluate and compare standard sperm parameters and sperm chromatin integrity by sperm chromatin dispersion test (SCD) in ejaculates from men whose partners have a history of recurrent pregnancy loss and from control group of fertile men.

Methods

Thirty couples with unexplained recurrent abortion (case group) and 30 fertile couples (control group) referring to Shiraz infertility center were included. Sperm parameters were assessed in semen samples from two groups and then staining with SCD procedure. The results were analyzed by performing ANOVA and Tukey^,s tests.

Results

In control group, nucleoids with big (65.93 ± 2.35), small (12.4 ± 0.60) and without halo (11.6 ± 0.50) showed significant difference with case group (41.40 ± 1.43), (21.16 ± 1.11) and (23.26 ± 1.10) respectively. In the RPL group spermatozoa with high percentage of abnormal parameters (morphology and motility) was observed (p ≤ 0.05).

Conclusion

This study strengthens the current literature associating sperm quality with recurrent pregnancy loss, and emphasizes the important of evaluating male factor by tests such as SCD in addition to conventional sperm parameters.

American Society for Reproductive Medicine (ASRM). Recurrent pregnancy loss. Patients Fact Sheet. Created February. 2005. Available at: http://www.asrm.org/patients/FactSheet/fac.html.Accessed January 9, 2008

Saxena D, Misro MM, Roy S, Chopra K, Sinha D, Nandan D, et al. Possible role of male factors in recurrent pregnancy loss. Indian J Physio Pharmacol. 2008;52(3):274–82.

Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update. 2003;9:331–45.PubMedCrossRef

Kobayashi T, Miyazaki T, Natori M, Nozava S. Protective role of superoxide dismutase in human sperm motility: superoxide dismutase activity and lipid peroxide in human semina; p;asma and spermatozoa. Human Reprod. 1991;6(7):983–6.

Carrel DT, Liu L, Peterson CM, Jones KP, Hatasaka HH, Erikson L, et al. Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch Androl. 2003;49:49–55.CrossRef

Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Flamigni C, et al. Sperm DAN fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod. 2006;21(11):2876–81.PubMedCrossRef

Puscheck EE, Jevendran RS. The impact of male factor on recurrent pregnancy loss. Curr Opin Obstet Gynecol. 2007;19:222–28.PubMedCrossRef

Fernandes JL, Muriel L, Rivero MT, Goyanes V, Vasquez R, Alvarez JG. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl. 2003;24:59–66.

Fernandes JL, Muriel L, Goyanes V, Segrelles E, Gosalves J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril. 2005;84:833–42.CrossRef

10.

World Health Organization. Laboratory manual for the examination of human semen and sperm cervical mucus interaction. 4th ed. Cambridge: Cambridge University Press; 1999.

11.

Kruger TF, Menkveld R, Stander FS, Lombard CJ, der Merwe JP Wan, van Zyl JA. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril. 1986;46.

12.

Zini A, Sigman M. Are tests of DNA damage clinically useful? Pros and Cons J of Androl. 2009;30:220–9.

13.

Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril. 1988;49:112–7.PubMed

14.

Hill JA, Abbott AF, Politch JA. Sperm morphology and recurrent abortion. Fertil Steril. 1994;9:1180–3.

15.

Kenneth F, Trifatter JR. Abnormal sperm morphology and recurrent pregnancy loss? Fertil Steril 2007

16.

Guzick DS, Overstreet GW, Factor-Litvak P, Brazil CK, Nakajima ST, et al. National Cooperative Reproductive Medicine Network. Sperm morphology, motility and concentration in infertile and fertile men. N Ingel J Med. 2001;345:1388–93.CrossRef

17.

Dada R. Recurrent pregnancy loss: Male factor. In: Deka D, Malhotra N, editors. An introduction to genetics and fetal medicine. New Delhi: Jaypee Publications; 2009. p. 31–7.

18.

Gopalkkrishnan K, Padwal V, Meherji PK. Poor quality of sperm as it affects repeated early pregnancy loss. Arch Androl. 2000;45:111–7.CrossRef

19.

Chen C, Lee S, Chen D, Chien H, Chen I, Chu Y. Apoptosis and kinematics of ejaculated spermatozoa in patients with varicocele. J Androl. 2004;25:348–53.PubMed

Title: Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion
Authors: Forouzan Absalan
Alireza Ghannadi
Marjaneh Kazerooni
Rostami Parifar
Fatemeh Jamalzadeh
Sahar Amiri
Publication date: 01-01-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 1/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9647-0

At a glance: The STEP trials

Springer Medicine

Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion

Abstract

Objective

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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