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Journal of Assisted Reproduction and Genetics

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01-01-2012 | Stem Cell Biology

Short-term in-vitro culture of goat enriched spermatogonial stem cells using different serum concentrations

Authors: M. Bahadorani, S. M. Hosseini, P. Abedi, M. Hajian, S. E. Hosseini, A. Vahdati, H. Baharvand, Mohammad H. Nasr-Esfahani

Published in: Journal of Assisted Reproduction and Genetics | Issue 1/2012

Abstract

Purpose

To investigate the effect of serum supplementing on short-term culture, fate determination and gene expression of goat spermatogonial stem cells (SSCs).

Methods

Crude testicular cells were plated over Datura-Stramonium Agglutinin (DSA) for 1 h, and non-adhering cells were cultured in the presence of different serum concentrations (1, 5, 10, and 15%) for 7 days in a highly enriched medium initially developed in mice. Colonies developed in each group were used for the assessment of morphology, immunocytochemistry, and gene expression.

Results

Brief incubation of testicular cells with DSA resulted in a significant increase in the number of cells that expressed the germ cell marker (VASA). The expression of THY1, a specific marker of undifferentiated spermatogonia, was significantly higher in colonies developed in the presence of 1% rather than 5, 10 and 15% serum.

Conclusion

Goat SSCs could proliferate and maintain in SSC culture media for 1 week at serum concentrations as low as 1%, while higher concentrations had detrimental effects on SSC culture/expansion.

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Title: Short-term in-vitro culture of goat enriched spermatogonial stem cells using different serum concentrations
Authors: M. Bahadorani
S. M. Hosseini
P. Abedi
M. Hajian
S. E. Hosseini
A. Vahdati
H. Baharvand
Mohammad H. Nasr-Esfahani
Publication date: 01-01-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 1/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9687-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Short-term in-vitro culture of goat enriched spermatogonial stem cells using different serum concentrations

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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