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Journal of Assisted Reproduction and Genetics

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01-01-2012 | genetics

Increased frequency of micronuclei in lymphocytes of infertile males after exposure to gamma irradiation: a possible sign of genomic instability

Authors: Sahar Moghbeli-Nejad, Hossein Mozdarani, Ashraf Aleyasin

Published in: Journal of Assisted Reproduction and Genetics | Issue 1/2012

Abstract

Purpose

Induced chromosomal instability and micronucleus (MN) formation in blood lymphocytes of infertile men in comparison with fertile men exposed to gamma radiation was investigated.

Methods

Blood samples of healthy and infertile donors were irradiated by 2 and 4 Gy Co-60 gamma-rays, then cultured in RPMI-1640 complete medium containing 1% phytoheamaglutinin (PHA) and incubated in a CO₂ incubator. Cytochalasin-B was added to the cultures at a final concentration of 4 μg/ml. Finally, harvesting, slide making, and analysis were performed according to standard procedures.

Results

We observed a statistically significant difference between the frequencies of micronuclei in lymphocytes of infertile individuals, compared to healthy donors, before and after exposure to gamma rays. Although higher in azoospermia patients, the frequency of MN was not statistically different between infertile groups.

Conclusions

This study indicates that genomic instability in infertile men could probably contribute to the development of an impaired reproductive capacity.

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Title: Increased frequency of micronuclei in lymphocytes of infertile males after exposure to gamma irradiation: a possible sign of genomic instability
Authors: Sahar Moghbeli-Nejad
Hossein Mozdarani
Ashraf Aleyasin
Publication date: 01-01-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 1/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9550-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Increased frequency of micronuclei in lymphocytes of infertile males after exposure to gamma irradiation: a possible sign of genomic instability

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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