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BMC Gastroenterology
Published in: BMC Gastroenterology 1/2019

Open Access 01-12-2019 | Ulcerative Colitis | Research article

The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation

Authors: Irina V. Tcymbarevich, Jyrki J. Eloranta, Jean-Benoît Rossel, Nicole Obialo, Marianne Spalinger, Jesus Cosin-Roger, Silvia Lang, Gerd A. Kullak-Ublick, Carsten A. Wagner, Michael Scharl, Klaus Seuwen, Pedro A. Ruiz, Gerhard Rogler, Cheryl de Vallière, Benjamin Misselwitz, on behalf of the Swiss IBD Cohort Study Group

Published in: BMC Gastroenterology | Issue 1/2019

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Abstract

Background

Tissue inflammation in inflammatory bowel diseases (IBD) is associated with a decrease in local pH. The gene encoding G-protein-coupled receptor 65 (GPR65) has recently been reported to be a genetic risk factor for IBD. In response to extracellular acidification, proton activation of GPR65 stimulates cAMP and Rho signalling pathways. We aimed to analyse the clinical and functional relevance of the GPR65 associated single nucleotide polymorphism (SNP) rs8005161.

Methods

1138 individuals from a mixed cohort of IBD patients and healthy volunteers were genotyped for SNPs associated with GPR65 (rs8005161, rs3742704) and galactosylceramidase (rs1805078) by Taqman SNP assays. 2300 patients from the Swiss IBD Cohort Study (SIBDC) were genotyped for rs8005161 by mass spectrometry based SNP genotyping. IBD patients from the SIBDC carrying rs8005161 TT, CT, CC and non-IBD controls (CC) were recruited for functional studies. Human CD14+ cells were isolated from blood samples and subjected to an extracellular acidic pH shift, cAMP accumulation and RhoA activation were measured.

Results

In our mixed cohort, but not in SIBDC patients, the minor variant rs8005161 was significantly associated with UC. In SIBDC patients, we observed a consistent trend in increased disease severity in patients carrying the rs8005161-TT and rs8005161-CT alleles. No significant differences were observed in the pH associated activation of cAMP production between IBD (TT, CT, WT/CC) and non-IBD (WT/CC) genotype carriers upon an acidic extracellular pH shift. However, we observed significantly impaired RhoA activation after an extracellular acidic pH shift in IBD patients, irrespective of the rs8005161 allele.

Conclusions

The T allele of rs8005161 might confer a more severe disease course in IBD patients. Human monocytes from IBD patients showed impaired pH associated RhoA activation upon an acidic pH shift.
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Metadata
Title
The impact of the rs8005161 polymorphism on G protein-coupled receptor GPR65 (TDAG8) pH-associated activation in intestinal inflammation
Authors
Irina V. Tcymbarevich
Jyrki J. Eloranta
Jean-Benoît Rossel
Nicole Obialo
Marianne Spalinger
Jesus Cosin-Roger
Silvia Lang
Gerd A. Kullak-Ublick
Carsten A. Wagner
Michael Scharl
Klaus Seuwen
Pedro A. Ruiz
Gerhard Rogler
Cheryl de Vallière
Benjamin Misselwitz
on behalf of the Swiss IBD Cohort Study Group
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Gastroenterology / Issue 1/2019
Electronic ISSN: 1471-230X
DOI
https://doi.org/10.1186/s12876-018-0922-8

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