Published in:
Open Access
01-12-2023 | Ulcerative Colitis | Research
Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Authors:
Yuxin Zhou, Gang Ji, Xiaoyi Yang, Zhenhua Chen, Liangliang Zhou
Published in:
BMC Gastroenterology
|
Issue 1/2023
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Abstract
Background
Clinical epidemiological studies have found that some patients with ulcerative colitis (UC) are prone to mental disorders. DSS-induced acute and chronic UC models are often used to evaluate the efficacy of anti-UC drugs. However, whether DSS has an effect on mouse behavior has not been reported.
Methods
Acute and chronic UC models were induced by 3% DSS and 1.5% DSS, respectively. The bloody stool, the changes in the colon length, and histopathological changes in the colon were used to evaluate the success of the animal model. The behavior of mice was evaluated by open field experiment, tail suspension experiment and Sucrose preference test.
Results
The weight of mice in 3% DSS group decreased significantly, the DAI score increased significantly, the colon length of mice was significantly shortened, and the structure of colonic crypts was abnormal, which showed inflammatory cell infiltration and shrinkage of crypts. Compared with the control group, the immobility time of 3%DSS group mice in the tail suspension test and forced swimming test had no effect, the number of running and grooming times was significantly reduced, and there was no significant difference in the number of standing times. No abnormality was observed in HE staining of the hippocampus. However, in 1.5% DSS-induced chronic UC model, behavioral and hippocampal abnormalities were observed not only UC symptoms.
Conclusions
The acute UC model induced by 3% DSS has certain influence on the
behavior of mice, but the mental state of mice is normal, which may be the abnormal
behavior caused by UC symptoms; However, the chronic UC model induced by 1.5%
DSS has a significant effect on the behavior of mice, and the mice have mental
disorders, which are caused by mental disorders.