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Respiratory Research
Published in: Respiratory Research 1/2011

Open Access 01-12-2011 | Research

Transcriptional landscape of bone marrow-derived very small embryonic-like stem cells during hypoxia

Authors: Sina A Gharib, Abdelnaby Khalyfa, Magdalena J Kucia, Ehab A Dayyat, Jinkwan Kim, Heather B Clair, David Gozal

Published in: Respiratory Research | Issue 1/2011

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Abstract

Background

Hypoxia is a ubiquitous feature of many lung diseases and elicits cell-specific responses. While the effects of hypoxia on stem cells have been examined under in vitro conditions, the consequences of in vivo oxygen deprivation have not been studied.

Methods

We investigated the effects of in vivo hypoxia on a recently characterized population of pluripotent stem cells known as very small embryonic-like stem cells (VSELs) by whole-genome expression profiling and measuring peripheral blood stem cell chemokine levels.

Results

We found that exposure to hypoxia in mice mobilized VSELs from the bone marrow to peripheral blood, and induced a distinct genome-wide transcriptional signature. Applying a computationally-intensive methodology, we identified a hypoxia-induced gene interaction network that was functionally enriched in a diverse array of programs including organ-specific development, stress response, and wound repair. Topographic analysis of the network highlighted a number of densely connected hubs that may represent key controllers of stem cell response during hypoxia and, therefore, serve as putative targets for altering the pathophysiologic consequences of hypoxic burden.

Conclusions

A brief exposure to hypoxia recruits pluripotent stem cells to the peripheral circulation and actives diverse transcriptional programs that are orchestrated by a selective number of key genes.
Appendix
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Metadata
Title
Transcriptional landscape of bone marrow-derived very small embryonic-like stem cells during hypoxia
Authors
Sina A Gharib
Abdelnaby Khalyfa
Magdalena J Kucia
Ehab A Dayyat
Jinkwan Kim
Heather B Clair
David Gozal
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Respiratory Research / Issue 1/2011
Electronic ISSN: 1465-993X
DOI
https://doi.org/10.1186/1465-9921-12-63

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Keynote webinar | Spotlight on medication adherence

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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