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Archives of Virology
Published in: Archives of Virology 11/2015

01-11-2015 | Brief Report

The silencing suppressor (NSs) protein of the plant virus Tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects

Authors: Virgínia Carla de Oliveira, Fabricio da Silva Morgado, Daniel Mendes Pereira Ardisson-Araújo, Renato Oliveira Resende, Bergmann Morais Ribeiro

Published in: Archives of Virology | Issue 11/2015

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Abstract

In this work, we showed that cell death induced by a recombinant (vAcNSs) Autographa californica multiple nucleopolyhedrovirus (AcMNPV) expressing the silencing suppressor (NSs) protein of Tomato spotted wilt virus (TSWV) was enhanced on permissive and semipermissive cell lines. The expression of a heterologous gene (firefly luciferase) during co-infection of insect cells with vAcNSs and a second recombinant baculovirus (vAgppolhfluc) was shown to increase when compared to single vAgppolhfluc infections. Furthermore, the vAcNSs mean time-to-death values were significantly lower than those for wild-type AcMNPV on larvae of Spodoptera frugiperda and Anticarsia gemmatalis. These results showed that the TSWV-NSs protein could efficiently increase heterologous protein expression in insect cells as well as baculovirus pathogenicity and virulence, probably by suppressing the gene-silencing machinery in insects.
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Metadata
Title
The silencing suppressor (NSs) protein of the plant virus Tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects
Authors
Virgínia Carla de Oliveira
Fabricio da Silva Morgado
Daniel Mendes Pereira Ardisson-Araújo
Renato Oliveira Resende
Bergmann Morais Ribeiro
Publication date
01-11-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2580-2

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