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Published in: Breast Cancer Research 3/2008

01-06-2008 | Letter

The future of mammary stem cell biology: the power of in vivo transplants

Authors: Geoffrey J Lindeman, Jane E Visvader, Matthew J Smalley, Connie J Eaves

Published in: Breast Cancer Research | Issue 3/2008

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Excerpt

The recent review by Smith and Medina [1] of in vivo transplantation models and their role in investigating mammary stem cell (MaSC) biology provides comprehensive coverage of the history and complexity of the 'gold standard' MaSC assay in mice. This includes a description of the pioneering studies that showed that mammary epithelial outgrowths can be generated in cleared mammary fat pads transplanted with explants or admixtures of mammary cells [2]. However, this approach clearly does not lend itself to prospective analysis of isolated subpopulations in order to identify which cells possess in vivo regenerative activity. More recently, success in obtaining complex mammary gland structures from transplanted suspensions of single cells has now made this possible [37]. Moreover, the regenerated structures have been shown to contain daughter cells with the same in vivo repopulating activity of the original stem cell transplanted [4, 6]. A major contribution from this advance has been the demonstration that the MaSCs thus defined are highly enriched in the CD49fhi/CD29hi/CD24+/mod/Sca-1- subset [46]. Nevertheless, it is important to recognize that these stem cells represent under 10% of this basal population. This population also contains mature myoepithelial cells and, in all likelihood, other basal cell intermediates that are yet to be identified. …
Literature
1.
2.
go back to reference Daniel CW, De Ome KB, Young JT, Blair PB, Faulkin LJ: The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study. Proc Natl Acad Sci USA. 1968, 61: 53-60. 10.1073/pnas.61.1.53.CrossRefPubMedPubMedCentral Daniel CW, De Ome KB, Young JT, Blair PB, Faulkin LJ: The in vivo life span of normal and preneoplastic mouse mammary glands: a serial transplantation study. Proc Natl Acad Sci USA. 1968, 61: 53-60. 10.1073/pnas.61.1.53.CrossRefPubMedPubMedCentral
3.
go back to reference Alvi AJ, Clayton H, Joshi C, Enver T, Ashworth A, Vivanco MM, Dale TC, Smalley MJ: Functional and molecular characterisation of mammary side population cells. Breast Cancer Res. 2003, 5: R1-R8. 10.1186/bcr563.CrossRefPubMed Alvi AJ, Clayton H, Joshi C, Enver T, Ashworth A, Vivanco MM, Dale TC, Smalley MJ: Functional and molecular characterisation of mammary side population cells. Breast Cancer Res. 2003, 5: R1-R8. 10.1186/bcr563.CrossRefPubMed
4.
go back to reference Shackleton M, Vaillant F, Simpson KJ, Stingl J, Smyth GK, Asselin-Labat ML, Wu L, Lindeman GJ, Visvader JE: Generation of a functional mammary gland from a single stem cell. Nature. 2006, 439: 84-88. 10.1038/nature04372.CrossRefPubMed Shackleton M, Vaillant F, Simpson KJ, Stingl J, Smyth GK, Asselin-Labat ML, Wu L, Lindeman GJ, Visvader JE: Generation of a functional mammary gland from a single stem cell. Nature. 2006, 439: 84-88. 10.1038/nature04372.CrossRefPubMed
5.
go back to reference Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ: CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006, 8: R7-10.1186/bcr1371.CrossRefPubMed Sleeman KE, Kendrick H, Ashworth A, Isacke CM, Smalley MJ: CD24 staining of mouse mammary gland cells defines luminal epithelial, myoepithelial/basal and non-epithelial cells. Breast Cancer Res. 2006, 8: R7-10.1186/bcr1371.CrossRefPubMed
6.
go back to reference Stingl J, Eirew P, Ricketson I, Shackleton M, Vaillant F, Choi D, Li HI, Eaves CJ: Purification and unique properties of mammary epithelial stem cells. Nature. 2006, 439: 993-997.PubMed Stingl J, Eirew P, Ricketson I, Shackleton M, Vaillant F, Choi D, Li HI, Eaves CJ: Purification and unique properties of mammary epithelial stem cells. Nature. 2006, 439: 993-997.PubMed
7.
go back to reference Welm BE, Tepera SB, Venezia T, Graubert TA, Rosen JM, Goodell MA: Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol. 2002, 245: 42-56. 10.1006/dbio.2002.0625.CrossRefPubMed Welm BE, Tepera SB, Venezia T, Graubert TA, Rosen JM, Goodell MA: Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population. Dev Biol. 2002, 245: 42-56. 10.1006/dbio.2002.0625.CrossRefPubMed
8.
go back to reference Sleeman KE, Kendrick H, Robertson D, Isacke CM, Ashworth A, Smalley MJ: Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland. J Cell Biol. 2007, 176: 19-26. 10.1083/jcb.200604065.CrossRefPubMedPubMedCentral Sleeman KE, Kendrick H, Robertson D, Isacke CM, Ashworth A, Smalley MJ: Dissociation of estrogen receptor expression and in vivo stem cell activity in the mammary gland. J Cell Biol. 2007, 176: 19-26. 10.1083/jcb.200604065.CrossRefPubMedPubMedCentral
9.
go back to reference Asselin-Labat ML, Sutherland KD, Barker H, Thomas R, Shackle-ton M, Forrest NC, Hartley L, Robb L, Grosveld FG, Wees van der J, Lindeman GJ, Visvader JE: Gata-3 is an essential regulator of mammary-gland morphogenesis and luminal-cell differentiation. Nat Cell Biol. 2007, 9: 201-209. 10.1038/ncb1530.CrossRefPubMed Asselin-Labat ML, Sutherland KD, Barker H, Thomas R, Shackle-ton M, Forrest NC, Hartley L, Robb L, Grosveld FG, Wees van der J, Lindeman GJ, Visvader JE: Gata-3 is an essential regulator of mammary-gland morphogenesis and luminal-cell differentiation. Nat Cell Biol. 2007, 9: 201-209. 10.1038/ncb1530.CrossRefPubMed
10.
go back to reference Asselin-Labat ML, Shackleton M, Stingl J, Vaillant F, Forrest NC, Eaves CJ, Visvader JE, Lindeman GJ: Steroid hormone receptor status of mouse mammary stem cells. J Natl Cancer Inst. 2006, 98: 1011-1014.CrossRefPubMed Asselin-Labat ML, Shackleton M, Stingl J, Vaillant F, Forrest NC, Eaves CJ, Visvader JE, Lindeman GJ: Steroid hormone receptor status of mouse mammary stem cells. J Natl Cancer Inst. 2006, 98: 1011-1014.CrossRefPubMed
Metadata
Title
The future of mammary stem cell biology: the power of in vivo transplants
Authors
Geoffrey J Lindeman
Jane E Visvader
Matthew J Smalley
Connie J Eaves
Publication date
01-06-2008
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue 3/2008
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr1986

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