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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Research

The effect of nanobased irrigants on the root canal dentin microhardness: an ex-vivo study

Authors: Safoora Sahebi, Hossein Mofidi, Abbas Abbaszadegan, Ahmad Gholami, Fateme Eskandari

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

Given the favorable antimicrobial properties of zinc oxide (ZnONPs), standard silver (AgNPs), and imidazolium-based silver (Im-AgNPs) nanoparticles, this study aimed to evaluate their influence on the microhardness of root canal dentin.

Methods

In this experimental study, 40 mandibular premolars were decoronated at the cementoenamel junction and longitudinally sectioned into halves to create 80 specimens. They were randomly allocated to 5 groups (n = 16) and irrigated with ZnONPs, AgNPs, Im-AgNPs, NaOCl, or normal saline (as the negative control) for 15 min. The Vickers Hardness Number (VHN) was measured on each root canal third before and after being soaked in irrigants. Statistical analysis was performed using paired t-test, one-way ANOVA, and post hoc Tukey’s test (α = 0.05).

Results

Im-AgNPs and ZnONPs irrigants improved the microhardness of root dentin, whereas, AgNPs and NaOCl decreased it. ZnONPs yielded the highest VHN at the coronal third (P˂0.001), while the Im-AgNPs provided the highest VHN at the middle and apical thirds (P˂0.001). The AgNPs group showed the lowest VHN at the apical third.

Conclusions

The irrigants containing Im-AgNPs and ZnONPs significantly enhanced the root dentin microhardness. However, the use of AgNPs resulted in decreased microhardness.
Literature
1.
Adl A, Razavian A, Eskandari F. The efficacy of EndoActivator, passive ultrasonic irrigation, and Ultra X in removing calcium hydroxide from root canals: an in-vitro study. BMC Oral Health. 2022;22(1):1–7.
2.
Eskandari F, Abbaszadegan A, Gholami A, Ghahramani Y. The antimicrobial efficacy of graphene oxide, double antibiotic paste, and their combination against Enterococcus faecalis in the root canal treatment. BMC Oral Health. 2023;23(1):1–10.
3.
Gonçalves LS, Rodrigues RCV, Junior CVA, Soares RG, Vettore MV. The effect of sodium hypochlorite and chlorhexidine as irrigant solutions for root canal disinfection: a systematic review of clinical trials. J Endod. 2016;42(4):527–32.PubMed
4.
Varshini R, Subha A, Prabhakar V, Mathini P, Narayanan S, Minu K. Antimicrobial efficacy of Aloe vera, lemon, Ricinus communis, and calcium hydroxide as intracanal medicament against Enterococcus faecalis: a confocal microscopic study. J Pharm Bioallied Sci. 2019;11(Suppl 2):256.
5.
Chan EL, Zhang C, Cheung GS. Cytotoxicity of a novel nano-silver particle endodontic irrigant. Clin Cosmet Investig Dent. 2015;7:65–74.PubMedPubMedCentral
6.
Generali L, Bertoldi C, Bidossi A, Cassinelli C, Morra M, Del Fabbro M, et al. Evaluation of cytotoxicity and antibacterial activity of a new class of silver citrate-based compounds as endodontic irrigants. Materials. 2020;13(21):5019–33.PubMedPubMedCentral
7.
Patel E, Pradeep P, Kumar P, Choonara YE, Pillay V. Oroactive dental biomaterials and their use in endodontic therapy. J Biomed Mater Res B Appl Biomater. 2020;108(1):201–12.PubMed
8.
Mohammadi Z, Shalavi S, Moeintaghavi A, Jafarzadeh H. A review over benefits and drawbacks of combining sodium hypochlorite with other endodontic materials. The Open Dentistry Journal. 2017;11:661–9.PubMedPubMedCentral
9.
Gherasim O, Puiu RA, Bîrcă AC, Burdușel A-C, Grumezescu AM. An updated review on silver nanoparticles in biomedicine. Nanomaterials. 2020;10(11):2318–62.PubMedPubMedCentral
10.
Sahebi S, Asheghi B, Samadi Y, Eskandari F. Effect of Calcium Hydroxide and Nano Calcium Hydroxide on push-out bond strength of Epoxy Resin Sealer to Root Canal dentin. Iran Endod J. 2022;17(1):13–9.PubMedPubMedCentral
11.
Abootalebi SN, Mousavi SM, Hashemi SA, Shorafa E, Omidifar N, Gholami A. Antibacterial effects of green-synthesized silver nanoparticles using Ferula asafoetida against Acinetobacter baumannii isolated from the hospital environment and assessment of their cytotoxicity on the human cell lines. J Nanomater. 2021;2021:1–12.
12.
Ebrahimi N, Rasoul-Amini S, Ebrahiminezhad A, Ghasemi Y, Gholami A, Seradj H. Comparative study on characteristics and cytotoxicity of bifunctional magnetic-silver nanostructures: synthesized using three different reducing agents. Acta Metall Sin (Engl Lett). 2016;29:326–34.
13.
Gholami A, Mousavi SM, Shomali A, Hashemi SA, Abootalebi SN, Chiang W-H et al. One-put Ferula-mediated synthesis of Biogenic Silver Nanoparticles with more Antimicrobial Effect and Promising Human Cell Biocompatibility. J Nanomaterials. 2022.
14.
Loyola-Rodríguez JP, Torres-Méndez F, Espinosa-Cristobal LF, García-Cortes JO, Loyola-Leyva A, González FJ, et al. Antimicrobial activity of endodontic sealers and medications containing chitosan and silver nanoparticles against Enterococcus faecalis. J Appl Biomater Funct Mater. 2019;17(3):2280800019851771.PubMed
15.
Abbaszadegan A, Nabavizadeh M, Gholami A, Aleyasin Z, Dorostkar S, Saliminasab M, et al. Positively charged imidazolium-based ionic liquid‐protected silver nanoparticles: a promising disinfectant in root canal treatment. Int Endod J. 2015;48(8):790–800.PubMed
16.
Farshad M, Abbaszadegan A, Ghahramani Y, Jamshidzadeh A. Effect of imidazolium-based silver nanoparticles on root dentin roughness in comparison with three common root canal irrigants. Iran Endod J. 2017;12(1):83–6.PubMedPubMedCentral
17.
Nabavizadeh M, Ghahramani Y, Abbaszadegan A, Jamshidzadeh A, Jenabi P, Makarempour A. In vivo biocompatibility of an ionic liquid-protected silver nanoparticle solution as root canal irrigant. Iran Endod J. 2018;13(3):293–8.PubMedPubMedCentral
18.
Abbaszadegan A, Nabavizadeh M, Gholami A, Aleyasin Z, Dorostkar S, Saliminasab M et al. Positively charged imidazolium-based ionic liquid‐protected silver nanoparticles: a promising disinfectant in root canal treatment. 2015;48(8):790–800.
19.
Chávez-Andrade GM, Tanomaru-Filho M, Rodrigues EM, Gomes-Cornélio AL, Faria G, Bernardi MIB et al. Cytotoxicity, genotoxicity and antibacterial activity of poly (vinyl alcohol)-coated silver nanoparticles and farnesol as irrigating solutions. 2017;84:89–93.
20.
Farshad M, Abbaszadegan A, Ghahramani Y, Jamshidzadeh AJIej. Effect of imidazolium-based silver nanoparticles on root dentin roughness in comparison with three common root canal irrigants. 2017;12(1):83.
21.
Nabavizadeh M, Ghahramani Y, Abbaszadegan A, Jamshidzadeh A, Jenabi P. Makarempour AJIej. In vivo biocompatibility of an ionic liquid-protected silver nanoparticle solution as root canal irrigant. 2018;13(3):293.
22.
Eskandari F, Mofidi H, Asheghi B, Mohammadi F, Gholami A. Bringing resistance modulation to methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) strains using a quaternary ammonium compound coupled with zinc oxide nanoparticles. World J Microbiol Biotechnol. 2023;39(7):193.PubMed
23.
Jowkar Z, Fattah Z, Ghanbarian S, Shafiei F. The effects of silver, zinc oxide, and titanium dioxide nanoparticles used as dentin pretreatments on the microshear bond strength of a conventional glass ionomer cement to dentin. Int J Nanomed. 2020;15:4755–62.
24.
Jowkar Z, Omidi Y, Shafiei F. The effect of silver nanoparticles, zinc oxide nanoparticles, and titanium dioxide nanoparticles on the push-out bond strength of fiber posts. J Clin Exp Dent. 2020;12(3):e249–e56.PubMedPubMedCentral
25.
Versiani MA, Abi Rached-Junior FJ, Kishen A, Pécora JD, Silva-Sousa YT, de Sousa-Neto MD. Zinc oxide nanoparticles enhance physicochemical characteristics of Grossman sealer. J Endod. 2016;42(12):1804–10.PubMed
26.
Jowkar Z, Hamidi SA, Shafiei F, Ghahramani Y. The effect of silver, zinc oxide, and titanium dioxide nanoparticles used as final irrigation solutions on the fracture resistance of root-filled teeth. Clin Cosmet Investig Dent. 2020;12:141–8.PubMedPubMedCentral
27.
Ari H, Erdemir A, Belli S. Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod. 2004;30(11):792–5.PubMed
28.
Aslantas EE, Buzoglu HD, Altundasar E, Serper A. Effect of EDTA, sodium hypochlorite, and chlorhexidine gluconate with or without surface modifiers on dentin microhardness. J Endod. 2014;40(6):876–9.PubMed
29.
Oliveira LD, Carvalho CAT, Nunes W, Valera MC, Camargo CHR, Jorge AOC. Effects of chlorhexidine and sodium hypochlorite on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;104(4):e125–e8.PubMed
30.
Bakr DK, Saleem SS, Amin BK. Effect of sodium hypochlorite, chlorhexidin and EDTA on dentin microhardness. Zanco J Med Sci. 2016;20(1):11759–9.
31.
Saha SG, Sharma V, Bharadwaj A, Shrivastava P, Saha MK, Dubey S, et al. Effectiveness of various endodontic irrigants on the micro-hardness of the root canal dentin: an in vitro study. J Clin Diagn Res. 2017;11(4):ZC01–ZC4.PubMedPubMedCentral
32.
Abbaszadegan A, Ghahramani Y, Gholami A, Hemmateenejad B, Dorostkar S, Nabavizadeh M, et al. The effect of charge at the surface of silver nanoparticles on antimicrobial activity against gram-positive and gram-negative bacteria: a preliminary study. J Nanomater. 2015;16(1):53.
33.
Abbaszadegan A, Gholami A, Abbaszadegan S, Aleyasin ZS, Ghahramani Y, Dorostkar S, et al. The effects of different ionic liquid coatings and the length of alkyl chain on antimicrobial and cytotoxic properties of silver nanoparticles. Iran Endod J. 2017;12(4):481.PubMedPubMedCentral
34.
Gholami A, Ghezelbash K, Asheghi B, Abbaszadegan A, Amini A. An in vitro study on the antibacterial effects of chlorhexidine-loaded positively charged silver nanoparticles on Enterococcus faecalis. Journal of Nanomaterials. 2022;2022.
35.
Gholami A, Shams MS, Abbaszadegan A, Nabavizadeh M. Ionic liquids as capping agents of silver nanoparticles. Part II: antimicrobial and cytotoxic study. Green Process Synthesis. 2021;10(1):585–93.
36.
Sahebi S, Sobhnamayan F, Moazami F, Naseri M. Assessment of sodium thiosulfate neutralizing effect on micro-hardness of dentin treated with sodium hypochlorite. BMC Oral Health. 2020;20(1):1–6.
37.
Shameli K, Ahmad MB, Jazayeri SD, Shabanzadeh P, Sangpour P, Jahangirian H, et al. Investigation of antibacterial properties silver nanoparticles prepared via green method. Chem Cent J. 2012;6(1):1–10.
38.
Shamhari NM, Wee BS, Chin SF, Kok KY. Synthesis and characterization of zinc oxide nanoparticles with small particle size distribution. Acta chimica Slovenica. 2018;65(3):578–85.PubMed
39.
Zak AK, Razali R, Abd Majid WH, Darroudi M. Synthesis and characterization of a narrow size distribution of zinc oxide nanoparticles. Int J Nanomed. 2011;6:1399–403.
40.
Kalishwaralal K, Deepak V, Ramkumarpandian S, Nellaiah H, Sangiliyandi G. Extracellular biosynthesis of silver nanoparticles by the culture supernatant of Bacillus licheniformis. Mater Lett. 2008;62(29):4411–3.
41.
Mehta B, Chhajlani M, Shrivastava B, editors. Green synthesis of silver nanoparticles and their characterization by XRD. Journal of physics: conference series; 2017: IOP Publishing.
42.
Sarkar R, Kumbhakar P, Mitra A. Green synthesis of silver nanoparticles and its optical properties. Dig J Nanomater Biostruct. 2010;5(2):491–6.
43.
Lodhi FL, Saleem MI, Aqib AI, Rashid I, Qureshi ZI, Anwar MA, et al. Bringing resistance modulation to epidemic methicillin resistant S. aureus of dairy through antibiotics coupled metallic oxide nanoparticles. Microb Pathog. 2021;159:105138.PubMed
44.
Ballal NV, Mala K, Bhat KS. Evaluation of the effect of maleic acid and ethylenediaminetetraacetic acid on the microhardness and surface roughness of human root canal dentin. J Endod. 2010;36(8):1385–8.PubMed
45.
Mofidi H, Khosravani SR, Garjani S, Mirghotbi T. Evaluate the efficacy of laser therapy in root canal disinfection with different irrigants protocols: a systematic review and meta-analysis. Eurasia J Biosci. 2020;14:4697–701.
46.
Sedigh-Shams M, Ebrahimi R, Asheghi B, Eskandari F. Comparative evaluation of cyclic fatigue resistance of two single-file rotary instruments in simulated stainless steel curved canals. J Res Dent Maxillofac Sci. 2022;7(1):48–54.
47.
Elika V, Kunam D, Anumula L, kumar Chinni S, Govula K. Comparative evaluation of Chloroquick with Triphala, sodium hypochlorite, and ethylenediaminetetraacetic acid on the microhardness of root canal dentin: an in vitro study. J Clin Transl Res. 2021;7(1):72–6.PubMedPubMedCentral
48.
Arslan H, Barutcigil C, Karatas E, Topcuoglu HS, Yeter KY, Ersoy I, et al. Effect of citric acid irrigation on the fracture resistance of endodontically treated roots. Eur J dentistry. 2014;8(01):074–8.
49.
Zhang Y-R, Du W, Zhou X-D, Yu H-Y. Review of research on the mechanical properties of the human tooth. Int J Oral Sci. 2014;6(2):61–9.PubMedPubMedCentral
50.
Ghisi AC, Kopper PMP, Baldasso FE, Stürmer CP, Rossi-Fedele G, Steier L, et al. Effect of super-oxidized water, sodium hypochlorite and EDTA on dentin microhardness. Braz Dent J. 2014;25:420–4.PubMed
51.
Pashley D, Okabe A, Parham P. The relationship between dentin microhardness and tubule density. Dent Traumatol. 1985;1(5):176–9.
52.
Angker L, Nockolds C, Swain MV, Kilpatrick N. Correlating the mechanical properties to the mineral content of carious dentine—a comparative study using an ultra-micro indentation system (UMIS) and SEM-BSE signals. Arch Oral Biol. 2004;49(5):369–78.PubMed
53.
Saghiri MA, Delvarani A, Mehrvarzfar P, Malganji G, Lotfi M, Dadresanfar B, et al. A study of the relation between erosion and microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108(6):e29–e34.PubMed
54.
Slutzky-Goldberg I, Maree M, Liberman R, Heling I. Effect of sodium hypochlorite on dentin microhardness. J Endod. 2004;30(12):880–2.PubMed
55.
Philip PM, Sindhu J, Poornima M, Naveen D, Nirupama D, Nainan MT. Effects of conventional and herbal irrigants on microhardness and flexural strength of root canal dentin: an in vitro study. J Conserv Dent. 2021;24(1):83–7.PubMedPubMedCentral
56.
Bosaid F, Aksel H, Makowka S, Azim A. Surface and structural changes in root dentine by various chelating solutions used in regenerative endodontics. Int Endod J. 2020;53(10):1438–45.PubMed
57.
Bhandi S, Mehta D, Mashyakhy M, Chohan H, Testarelli L, Thomas J, et al. Antimicrobial efficacy of silver nanoparticles as root canal irrigant’s: a systematic review. J Clin Med. 2021;10(6):1152.PubMedPubMedCentral
58.
Charannya S, Duraivel D, Padminee K, Poorni S, Nishanthine C, Srinivasan MR. Comparative evaluation of antimicrobial efficacy of silver nanoparticles and 2% chlorhexidine gluconate when used alone and in combination assessed using agar diffusion method: an in vitro study. Contemp Clin Dent. 2018;9(Suppl 2):204–S9.
59.
Suzuki TYU, Gallego J, Assunção WG, Briso ALF, Dos Santos PH. Influence of silver nanoparticle solution on the mechanical properties of resin cements and intrarradicular dentin. PLoS ONE. 2019;14(6):e0217750.PubMedPubMedCentral
60.
Hassan R, Khallaf M. Effect of a silver nanoparticle intracanal-based medicament on the microhardness of human radicular dentine. Endod Pract Today. 2018;12:125–7.
61.
Baras BH, Sun J, Melo MAS, Tay FR, Oates TW, Zhang K, et al. Novel root canal sealer with dimethylaminohexadecyl methacrylate, nano-silver and nano-calcium phosphate to kill bacteria inside root dentin and increase dentin hardness. Dent Mater. 2019;35(10):1479–89.PubMed
62.
Adl A, Abbaszadegan A, Gholami A, Parvizi F, Ghahramani Y. Effect of a new imidazolium-based silver nanoparticle irrigant on the bond strength of epoxy resin sealer to root canal dentine. Iran Endod J. 2019;14(2):122–5.PubMedPubMedCentral
63.
Patra JK, Baek K-H. Green nanobiotechnology: factors affecting synthesis and characterization techniques. J Nanomater. 2014;2014:1–13.
64.
Zhu J, Liang R, Sun C, Xie L, Wang J, Leng D, et al. Effects of nanosilver and nanozinc incorporated mesoporous calcium-silicate nanoparticles on the mechanical properties of dentin. PLoS ONE. 2017;12(8):e0182583.PubMedPubMedCentral
65.
Al-Naimi RJ, Al-Alousi WS, Al-Nema M. Effect of silver and zinc oxide nanoparticle addition on microhardness and depth of cure of resin based pit and fissure sealants. Int J Enhanc Res Sci Technol Eng. 2015;4(4):157–63.
Metadata
Title
The effect of nanobased irrigants on the root canal dentin microhardness: an ex-vivo study
Authors
Safoora Sahebi
Hossein Mofidi
Abbas Abbaszadegan
Ahmad Gholami
Fateme Eskandari
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03298-z

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