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BMC Complementary Medicine and Therapies

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Open Access 01-12-2020 | Research article

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H₂O₂) in human embryonic kidney 293 T cells

Authors: Yanyan Li, Yunyi Li, Zhie Fang, Dan Huang, Yalin Yang, Dijia Zhao, Mingchun Hang, Junda Wang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

In this study, Malus doumeri leaf flavonoids (MDLF) were used as the research object to observe their in vitro antioxidant stress ability. Hydrogen peroxide (H₂O₂) was used to induce oxidative stress in 293 T cells.

Methods

MTT, flow cytometry, and qPCR were used to verify the effect of MDLF.

Results

In vitro cell experiments showed that at a concentration of 0–160 μg/mL, MDLF did not affect the normal proliferation of human embryonic kidney 293 T cells (HEK 293 T cells), and MDLF had no cytotoxic effect in this concentration range. It was found that MDLF could maintain the survival of HEK 293 T cells (82.6%) at a high concentration (160 μg/mL). Morphological observation also found that MDLF can inhibit the cell structure imperfection caused by H₂O₂. It was also observed that MDLF could significantly increase the levels of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-Px) and reduce the level of malondialdehyde (MDA). The results of quantitative polymerase chain reaction (qPCR) showed that MDLF could significantly up-regulate the mRNA expression levels of CAT, SOD, GSH, GSH-Px, B-cell lymphoma-2 (Bcl-2) and downregulate the expression levels of B-cell lymphoma-2 associated x protein (Bax), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa-B (NF-κB) in oxidative stress-injured cells. The HPLC analysis showed that MDLF contained hyperin, isoquercetin, quercitrin, hesperidin, myricetin, baicalin and quercetin.

Conclusion

From the experimental results, it was observed that MDLF has a strong anti-oxidation ability in vitro, and it can interfere with the oxidative stress damage caused by H₂O₂ in 293 T cells. Therefore, MDLF is a type of natural substance with good anti-oxidant effect, and it has the potential to interfere with many diseases.

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Title: The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H2O2) in human embryonic kidney 293 T cells
Authors: Yanyan Li
Yunyi Li
Zhie Fang
Dan Huang
Yalin Yang
Dijia Zhao
Mingchun Hang
Junda Wang
Publication date: 01-12-2020
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-03072-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The effect of Malus doumeri leaf flavonoids on oxidative stress injury induced by hydrogen peroxide (H₂O₂) in human embryonic kidney 293 T cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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