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BMC Complementary Medicine and Therapies

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Open Access 01-12-2020 | Research article

Schisandra chinensis bee pollen’s chemical profiles and protective effect against H₂O₂-induced apoptosis in H9c2 cardiomyocytes

Authors: Peiying Shi, Qianqian Geng, Lifu Chen, Tianyu Du, Yan Lin, Rongcai Lai, Fei Meng, Zhenhong Wu, Xiaoqing Miao, Hong Yao

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

Schisandra chinensis (Turcz.) Baill bee pollen extract (SCBPE) is often used as a functional food in China due to its good antioxidant property. However, its chemical compositions and effects on H9c2 cardiomyocytes against H₂O₂-induced cell injury still lacks of reports thus far. This study aimed to characterize the main components of SCBPE and investigate its protective effects against H₂O₂-induced H9c2 cardiomyocyte injury.

Methods

The main components of SCBPE were analyzed via ultraperformance liquid chromatography–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF MS/MS). The three main nucleosides in SCBPE were quantitatively analyzed via ultraperformance liquid chromatography–diode array detection. Furthermore, the potential mechanism by which SCBPE exerts protective effects against H₂O₂-induced H9c2 cardiomyocyte injury was explored for the first time via cell survival rate measurements; cell morphological observation; myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) and glutathione (GSH) level determination; flow cytometry; and quantitative polymerase chain reaction.

Results

Two carbohydrates, three nucleosides, and nine quinic acid nitrogen-containing derivatives in SCBPE were identified or tentatively characterized via UPLC–QTOF MS/MS. The nine quinic acid nitrogen-containing derivatives were first reported in bee pollen. The contents of uridine, guanosine, and adenosine were 2.4945 ± 0.0185, 0.1896 ± 0.0049, and 1.8418 ± 0.0157 μg/mg, respectively. Results of in vitro experiments showed that cell survival rate, myocardial SOD activity, and GSH level significantly increased and myocardial MDA level significantly decreased in SCBPE groups compared with those in H₂O₂ group. Cell morphology in SCBPE groups also markedly improved compared with that in H₂O₂ group. Results indicated that SCBPE protected H9c2 cardiomyocytes from H₂O₂-induced apoptosis by downregulating the mRNA expressions of Bax, cytochrome C, and caspase-3 and upregulating the Bcl-2 mRNA expression.

Conclusions

This study is the first to report that SCBPE could protect against oxidative stress injury and apoptosis in H₂O₂-injured H9c2 cells. Results indicated that the nucleosides and quinic acid nitrogen-containing derivatives could be the main substances that exert protective effects against H₂O₂-induced H9c2 cardiomyocyte injury.

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Title: Schisandra chinensis bee pollen’s chemical profiles and protective effect against H2O2-induced apoptosis in H9c2 cardiomyocytes
Authors: Peiying Shi
Qianqian Geng
Lifu Chen
Tianyu Du
Yan Lin
Rongcai Lai
Fei Meng
Zhenhong Wu
Xiaoqing Miao
Hong Yao
Publication date: 01-12-2020
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-03069-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Schisandra chinensis bee pollen’s chemical profiles and protective effect against H₂O₂-induced apoptosis in H9c2 cardiomyocytes

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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