Published in:
Open Access
01-05-2014 | Oral presentation
Systems biology analysis of immune signaling in peripheral blood mononuclear cells (PBMC) of melanoma patients receiving ipilimumab; basis for response biomarker identification
Authors:
Drew Hotson, Ryan Alvarado, Andy Conroy, Santosh Putta, Ester Simeone, Assunta Esposito, Mariaelena Capone, Gabriele Madonna, Antonio M Grimaldi, David B Rosen, Spencer Liang, Alessandra Cesano, Carmela Cacciapuoti, Paolo A Ascierto, Rachael E Hawtin
Published in:
Journal of Translational Medicine
|
Special Issue 1/2014
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Excerpt
Ipilimumab, an anti-CTLA-4 monoclonal antibody, is approved for treatment of unresectable/metastatic melanoma [
1]. Treatment benefits only a subset of patients (pts) and is associated with significant adverse effects. Biomarkers of pt response are needed. Single cell network profiling (SCNP) is a multiparametric flow cytometry-based assay that quantitatively measures both phenotypic markers and changes in intracellular signaling proteins in response to modulation, enabling analysis of cell signaling networks [
2,
3]. We have been concerned on functional proteomic profiling of immune signaling pathways in PBMC subsets in healthy donors and pts with metastatic melanoma receiving ipilumumab treatment. …
