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International Journal of Implant Dentistry

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Open Access 01-12-2020 | Research

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

Authors: Maria Alejandra Frias Martinez, Ísis de Fátima Balderrama, Paula Stephania Brandão Hage Karam, Rodrigo Cardoso de Oliveira, Flávia Amadeu de Oliveira, Carlos Roberto Grandini, Fábio Bossoi Vicente, Andreas Stavropoulos, Mariana Schutzer Ragghianti Zangrando, Adriana Campos Passanezi Sant’Ana

Published in: International Journal of Implant Dentistry | Issue 1/2020

Abstract

Purpose

The aim of this study was to investigate the response of osteogenic cell lineage and gingival fibroblastic cells to different surface treatments of grade IV commercially pure Titanium (cpTi) disks.

Material and methods

Grade IV cpTi disks with different surfaces were produced: machined (M), sandblasting (B), sandblasting and acid subtraction (NP), and hydrophilic treatment (ACQ). Surface microtopography characteristics and chemical composition were investigated by scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS). Adhesion and proliferation of SC-EHAD (human surgically-created early healing alveolar defects) and HGF-1 (human gingival fibroblasts) on Ti disks were investigated at 24 and 48 h, and osteogenic differentiation and mineralization were evaluated by assessing alkaline phosphatase (ALP) activity and alizarin red staining, respectively.

Results

No significant differences were found among the various surface treatments for all surface roughness parameters, except for skewness of the assessed profile (Rsk) favoring M (p = 0.035 ANOVA). M disks showed a slightly higher (p > 0.05; Kruskal-Wallis/Dunn) adhesion of HGF-1 (89.43 ± 9.13%) than SC-EHAD cells (57.11 ± 17.72%). ACQ showed a significantly higher percentage of SC-EHAD (100%) than HGF-1 (69.67 ± 13.97%) cells adhered at 24 h. SC-EHAD cells expressed increased ALP activity in osteogenic medium at M (213%) and NP (235.04%) surfaces, but higher mineralization activity on ACQ (54.94 ± 4.80%) at 14 days.

Conclusion

These findings suggest that surface treatment influences the chemical composition and the adhesion and differentiation of osteogenic cells in vitro.

Clinical relevance

Hydrophilic surface treatment of grade IV cpTi disks influences osteogenic cell adhesion and differentiation, which might enhance osseointegration.

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Title: Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human
Authors: Maria Alejandra Frias Martinez
Ísis de Fátima Balderrama
Paula Stephania Brandão Hage Karam
Rodrigo Cardoso de Oliveira
Flávia Amadeu de Oliveira
Carlos Roberto Grandini
Fábio Bossoi Vicente
Andreas Stavropoulos
Mariana Schutzer Ragghianti Zangrando
Adriana Campos Passanezi Sant’Ana
Publication date: 01-12-2020
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Implant Dentistry / Issue 1/2020
Electronic ISSN: 2198-4034
DOI: https://doi.org/10.1186/s40729-020-00243-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Surface roughness of titanium disks influences the adhesion, proliferation and differentiation of osteogenic properties derived from human

Abstract

Purpose

Material and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Material and methods

Results

Conclusion

Clinical relevance

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