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01-06-2013

Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor

Authors: Lynnae Schwartz, Sergei V. Spitsin, John Meshki, Florin Tuluc, Steven D. Douglas, John H. Wolfe

Published in: Journal of NeuroVirology | Issue 3/2013

Abstract

The associations between the neurokinin-1 receptor (NK-1R), substance P (SP), and HIV-1 were investigated in neurosphere-derived cultures of microglial-depleted human fetal brain cells (HFBC). Full-length NK-1R was identified in HFBC cultures. SP treatment of the HFBC increased intracellular calcium mobilization and decreased electrical impedance, both of which were blocked by the NK-1R antagonist aprepitant. SP treatment of HIV-1-infected HFBC upregulated HIV-1 expression. These data show that human neural cells grown from neurospheres express functional full length NK-1R that is responsive to SP, and that SP enhanced HIV-1 infection in HBFC.

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Title: Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor
Authors: Lynnae Schwartz
Sergei V. Spitsin
John Meshki
Florin Tuluc
Steven D. Douglas
John H. Wolfe
Publication date: 01-06-2013
Publisher: Springer US
Published in: Journal of NeuroVirology / Issue 3/2013
Print ISSN: 1355-0284
Electronic ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-013-0166-x

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Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor

Abstract

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Abstract

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