Use of immunological methods to the detection of toxoplasmosis and heat shock protein HSP70 in men

Toxoplasmosis is a contagious infection caused by the parasite Toxoplasma gondii, affecting many different body organs, and its effect varies depending on the location of the infection and the condition of the host’s immune system, this study was the first experiment in Al-Qadisiyah governorate to detect toxoplasmosis and thermal shock protein in males. 200 male students between the ages of 18–23 years at University of Al-Qadisiyah were persuaded to perform the examination, the immunological method ELISA showed to presence of T. gondii in 39/200 (19.5%), (10%) positive IgG antibodies and (6%) positive for IgM, statistical analysis indicates that there are significant differences between antibody presence rates between control and infection group, due to the inaccuracy of serological tests in some cases due to the reliance on the presence of antibodies, the present study used molecular methods to examine the samples again as a step to confirmation and to reach the most accurate results, depending on the gene B1 special for the parasite by using real-time PCR technique, and the results indicated a positive proportion was 15/200 (7.5%) as modern infection, the study also assessed the level of the heat shock protein HPS70 in the positive samples of infection and showed a high concentration of HPS70 in the blood samples for infected males (28.21) ng/ml compared with control group (6.96) ng/ml, statistical analysis showed significant differences between them.