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Virology Journal
Published in: Virology Journal 1/2015

Open Access 01-12-2015 | Research

Sequencing and phylogenetic analysis of the gp51 gene from Korean bovine leukemia virus isolates

Authors: EunJung Lee, Eun-Ju Kim, Ha-Kyung Joung, Bo-Hye Kim, Jae-Young Song, In-Soo Cho, Kyoung-Ki Lee, Yeun-Kyung Shin

Published in: Virology Journal | Issue 1/2015

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Abstract

Background

Bovine Leukemia virus (BLV) infection of cattle has been reported in Korea for more than three decades. However, to date, there have been few studies regarding Korean BLV since 1980s. Thus, the purpose of this study is to perform a diagnosis and molecular characterization of BLV strains circulating in Korea and to estimate genetic diversity of different genotypes of BLV.

Method

To investigate the distribution of BLV variants in the world and assess the evolutionary history of Korean BLV isolates, a comprehensive molecular analysis of the BLV env gp51 gene was conducted using recent worldwide BLV isolates. The isolates included 50 samples obtained from two cattle farms in southeastern Korea in 2014.

Results

Sequence and phylogenetic analyses of partial 444-nt fragment sequences and complete gp51 sequences of BLV revealed eight distinct genotypes of BLV showing geographic distribution of the world. Most Korean BLV isolates were found to belong to genotype 1 which is a major genotype prevailed throughout the world, and only four isolates from one farm were classified as genotype 3 related to the US and Japan isolates. Analysis of amino acids of Korean BLV isolates showed several sequence substitutions in the leader peptide, conformational epitope, and neutralizing domain regions. The observations suggest the possibility of affecting on viral infectivity and formation.

Conclusion

Korean BLV isolates showed the close relationship to genotype 1 and 3. Further study to identify the diversity of BLV circulating in Korea is necessary with samples collected nationwide because this study is the first report of BLV genotype 3 being in circulation in Korea.
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Metadata
Title
Sequencing and phylogenetic analysis of the gp51 gene from Korean bovine leukemia virus isolates
Authors
EunJung Lee
Eun-Ju Kim
Ha-Kyung Joung
Bo-Hye Kim
Jae-Young Song
In-Soo Cho
Kyoung-Ki Lee
Yeun-Kyung Shin
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2015
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-015-0286-4

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