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01-09-2016 | Original Contribution

Rice bran prevents high-fat diet-induced inflammation and macrophage content in adipose tissue

Authors: Maria Luisa Justo, Carmen Claro, Maximilian Zeyda, Thomas M. Stulnig, María Dolores Herrera, Rosalía Rodríguez-Rodríguez

Published in: European Journal of Nutrition | Issue 6/2016

Abstract

Background

The inflammatory process associated with obesity mainly arises from white adipose tissue (WAT) alterations. In the last few years, nutritional-based strategies have been positioned as promising alternatives to pharmacological approaches against these pathologies. Our aim was to determine the potential of a rice bran enzymatic extract (RBEE)-supplemented diet in the prevention of metabolic, biochemical and functional adipose tissue and macrophage changes associated with a diet-induced obesity (DIO) in mice.

Methods

C57BL/6J mice were fed high-fat diet (HF), 1 and 5 % RBEE-supplemented high-fat diet (HF1 % and HF5 %, respectively) and standard diet as control. Serum cardiometabolic parameters, adipocytes size and mRNA expression of pro-inflammatory biomarkers and macrophage polarization-related genes from WAT and liver were evaluated.

Results

RBEE administration significantly decreased insulin resistance in obese mice. Serum triglycerides, total cholesterol, glucose, insulin, adiponectin and nitrites from treated mice were partially restored, mainly by 1 % RBEE-enriched diet. The incremented adipocytes size observed in HF group was reduced by RBEE treatment, being 1 % more effective than 5 % RBEE. Pro-inflammatory biomarkers in WAT such as IL-6 and IL-1β were significantly decreased in RBEE-treated mice. Adiponectin, PPARγ, TNF-α, Emr1 or M1/M2 levels were significantly restored in WAT from HF1 % compared to HF mice.

Conclusions

RBEE-supplemented diet attenuated insulin resistance, dyslipidemia and morphological and functional alterations of adipose tissue in DIO mice. These benefits were accompanied by a modulating effect in adipocytes secretion and some biomarkers associated with macrophage polarization. Therefore, RBEE may be considered an alternative nutritional complement over metabolic syndrome and its complications.

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Title: Rice bran prevents high-fat diet-induced inflammation and macrophage content in adipose tissue
Authors: Maria Luisa Justo
Carmen Claro
Maximilian Zeyda
Thomas M. Stulnig
María Dolores Herrera
Rosalía Rodríguez-Rodríguez
Publication date: 01-09-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 6/2016
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-015-1015-x

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