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Malaria Journal
Published in: Malaria Journal 1/2009

Open Access 01-12-2009 | Research

Plasmodium falciparum enolase: stage-specific expression and sub-cellular localization

Authors: Ipsita Pal Bhowmick, Nirbhay Kumar, Shobhona Sharma, Isabelle Coppens, Gotam K Jarori

Published in: Malaria Journal | Issue 1/2009

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Abstract

Background

In an earlier study, it was observed that the vaccination with Plasmodium falciparum enolase can confer partial protection against malaria in mice. Evidence has also build up to indicate that enolases may perform several non-glycolytic functions in pathogens. Investigating the stage-specific expression and sub-cellular localization of a protein may provide insights into its moonlighting functions.

Methods

Sub-cellular localization of P. falciparum enolase was examined using immunofluorescence assay, immuno-gold electron microscopy and western blotting.

Results

Enolase protein was detected at every stage in parasite life cycle examined. In asexual stages, enolase was predominantly (≥85–90%) present in soluble fraction, while in sexual stages it was mostly associated with particulate fraction. Apart from cytosol, enolase was found to be associated with nucleus, food vacuole, cytoskeleton and plasma membrane.

Conclusion

Diverse localization of enolase suggests that apart from catalyzing the conversion of 2-phosphoglycericacid into phosphoenolpyruvate in glycolysis, enolase may be involved in a host of other biological functions. For instance, enolase localized on the merozoite surface may be involved in red blood cell invasion; vacuolar enolase may be involved in food vacuole formation and/or development; nuclear enolase may play a role in transcription.
Appendix
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Metadata
Title
Plasmodium falciparum enolase: stage-specific expression and sub-cellular localization
Authors
Ipsita Pal Bhowmick
Nirbhay Kumar
Shobhona Sharma
Isabelle Coppens
Gotam K Jarori
Publication date
01-12-2009
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2009
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/1475-2875-8-179

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