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01-06-2021 | Photodynamic Therapy | Original Article

Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy

Authors: Kumiko Koibuchi Sakane, Tanmoy Bhattacharjee, Jaciara Fagundes, Luciana Maria Cortez Marcolino, Isabelle Ferreira, Juliana Guerra Pinto, Juliana Ferreira-Strixino

Published in: Lasers in Medical Science | Issue 4/2021

Abstract

Photodynamic therapy (PDT) with photosensitizer methylene blue was applied to Leishmania braziliensis, and Fourier transform infrared (FTIR) spectroscopy was used to study biochemical changes in the parasite after PDT in comparison to untreated (C), only irradiation (I), and only photosensitizer (PS). Spectral analysis suggests increase in lipids, proteins, and protein secondary structures in PDT compared with C and decrease in nucleic acids and carbohydrates. Interestingly, these trends are different from PDT of Leishmania major species, wherein lipids decrease; there are minimal changes in secondary structures and increase in nucleic acids and carbohydrates. The study thus suggests possibility of different biomolecular players/pathways in PDT-induced death of L. braziliensis and L. major.

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Title: Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy
Authors: Kumiko Koibuchi Sakane
Tanmoy Bhattacharjee
Jaciara Fagundes
Luciana Maria Cortez Marcolino
Isabelle Ferreira
Juliana Guerra Pinto
Juliana Ferreira-Strixino
Publication date: 01-06-2021
Publisher: Springer London
Keywords: Photodynamic Therapy
Leishmania
Leishmania
Published in: Lasers in Medical Science / Issue 4/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03110-2

At a glance: The STEP trials

Springer Medicine

Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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